LOCUS       sequence-324084-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of N' Sialyl Transferase tagged with EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Patterson GH, Hirschberg K, Polishchuk RS, Gerlich D, Phair RD, 
            Lippincott-Schwartz J
  TITLE     Transport through the Golgi apparatus by rapid partitioning within a
            two-phase membrane system.
  JOURNAL   Cell. 2008 Jun 13;133(6):1055-67. doi: 10.1016/j.cell.2008.04.044.
  PUBMED    18555781
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2008.04"; journalName: "Cell"; date: "2008-06-13- 
            13"; volume: "133"; issue: "6"; pages: "1055-67"
COMMENT     Sequence Label: SiT-EGFP
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      261..270
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(267..269,270..272,273..785)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(312..333)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(573..592)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     920..941
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cggtgggagg tctatataag cagagctggt ttagtgaacc gtcagatccg ctagcgctac
       61 cggactcaga tctcgagctc gccaccatga ttcacaccaa cctgaagaaa aagttcagct
      121 gctgcgtcct ggtctttctt ctgtttgcag tcatctgtgt gtggaaggaa aagaagaaag
      181 ggagttacta tgattccttt aaattgcaaa ccaaggaatt cctgcagtcg acggtaccgc
      241 gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
      301 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      361 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      421 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      481 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      541 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      601 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      661 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      721 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      781 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      841 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc
      901 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      961 tcggcatg
//
