LOCUS       sequence-324196-       10560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian CG-to-GC base editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Koblan LW, Arbab M, Shen MW, Hussmann JA, Anzalone AV, Doman JL, 
            Newby GA, Yang D, Mok B, Replogle JM, Xu A, Sisley TA, Weissman JS, 
            Adamson B, Liu DR
  TITLE     Efficient C*G-to-G*C base editors developed using CRISPRi screens, 
            target-library analysis, and machine learning.
  JOURNAL   Nat Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z.
  PUBMED    34183861
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2021 Jun 28. pii: 10.1038/s41587-021-00938-z. doi: 
            10.1038/s41587-021-00938-z."
COMMENT     Sequence Label: pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX
FEATURES             Location/Qualifiers
     source          1..10560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             445..465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2692..6792
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7546..7566
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7575..7592
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7615..7632)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7621..7845
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7916..7932)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7916..7932)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7929..7951)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7940..7956
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7964..7970,7971..7988,7989..7994))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8009..8030
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8147..8164)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8318..8906)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8398..8417)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9077..9868,9869..9937))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9700..9719
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9938..10042)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10121..10140)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10312..10560,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcatggc cggagcacag
      481 gattttgtcc cacatactgc cgatctggct gagctggctg ccgccgctgg agagtgtaga
      541 ggatgcgggc tgtaccggga tgccacacag gccgtgttcg gagcaggcgg caggagcgcc
      601 cgcatcatga tgatcggcga gcagccaggc gataaggagg acctggccgg cctgccattt
      661 gtgggaccag caggccggct gctggacaga gccctggagg ccgccgacat cgatagggac
      721 gccctgtacg tgaccaacgc cgtgaagcac ttcaagttta cacgcgccgc aggaggcaag
      781 aggagaatcc acaagacccc ctctcggaca gaggtggtgg cctgcagacc ttggctgatc
      841 gccgagatga ccagcgtgga gccagatgtg gtggtgctgc tgggagcaac agcagcaaag
      901 gccctgctgg gcaatgactt cagggtgacc cagcacaggg gagaggtgct gcacgtggac
      961 gatgtgccag gcgatcctgc cctggtggca accgtgcacc ctagctccct gctgagaggc
     1021 ccaaaggagg agcgggaatc cgccttcgct gggctggtgg acgacctgag agtggctgcc
     1081 gatgtgagac cttctggagg atctagcgga ggctcctctg gcagcgagac accaggaaca
     1141 agcgagtcag caacaccaga gagcagtggc ggcagcagcg gggggtcatc ctcagagact
     1201 gggcctgtcg ccgtcgatcc aaccctgcgc cgccggattg aacctcacga gtttgaagtg
     1261 ttctttgacc cccgggagct gagaaaggag acatgcctgc tgtacgagat caactgggga
     1321 ggcaggcact ccatctggag gcacacctct cagaacacaa ataagcacgt ggaggtgaac
     1381 ttcatcgaga agtttaccac agagcggtac ttctgcccca ataccagatg tagcatcaca
     1441 tggtttctga gctggtcccc ttgcggagag tgtagcaggg ccatcaccga gttcctgtcc
     1501 agatatccac acgtgacact gtttatctac atcgccaggc tgtatcacca cgcagaccca
     1561 gagaataggc agggcctgga ggatctgatc agctccggcg tgaccatcca gatcatgaca
     1621 gagcaggagt ccggctactg ctggcggaac ttcgtgaatt attctcctag caacgaggcc
     1681 cactggccta ggtacccaca cctgtgggtg cgcctgtacg tgctggagct gtattgcatc
     1741 atcctgggcc tgcccccttg tctgaatatc ctgcggagaa agcagcccca gctgaccttc
     1801 tttacaatcg ccctgcagtc ttgtcactat cagaggctgc caccccacat cctgtgggcc
     1861 acaggcctga agtctggagg atctagcgga ggatcctctg gcagcgagac accaggaaca
     1921 agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcat ggccggagca
     1981 caggattttg tcccacatac tgccgatctg gctgagctgg ctgccgccgc tggagagtgt
     2041 agaggatgcg ggctgtaccg ggatgccaca caggccgtgt tcggagcagg cggcaggagc
     2101 gcccgcatca tgatgatcgg cgagcagcca ggcgataagg aggacctggc cggcctgcca
     2161 tttgtgggac cagcaggccg gctgctggac agagccctgg aggccgccga catcgatagg
     2221 gacgccctgt acgtgaccaa cgccgtgaag cacttcaagt ttacacgcgc cgcaggaggc
     2281 aagaggagaa tccacaagac cccctctcgg acagaggtgg tggcctgcag accttggctg
     2341 atcgccgaga tgaccagcgt ggagccagat gtggtggtgc tgctgggagc aacagcagca
     2401 aaggccctgc tgggcaatga cttcagggtg acccagcaca ggggagaggt gctgcacgtg
     2461 gacgatgtgc caggcgatcc tgccctggtg gcaaccgtgc accctagctc cctgctgaga
     2521 ggcccaaagg aggagcggga atccgccttc gctgggctgg tggacgacct gagagtggct
     2581 gccgatgtga gacctagcgg tggtagttct ggcggtagct caggatctga aactcccggc
     2641 acatctgaaa gcgcgactcc cgaatcatct ggtggatctt caggtggaag tgacaagaag
     2701 tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag
     2761 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
     2821 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
     2881 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
     2941 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
     3001 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
     3061 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
     3121 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
     3181 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     3241 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
     3301 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
     3361 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
     3421 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
     3481 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     3541 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
     3601 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
     3661 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
     3721 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
     3781 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
     3841 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
     3901 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
     3961 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
     4021 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
     4081 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
     4141 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
     4201 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
     4261 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
     4321 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
     4381 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
     4441 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
     4501 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
     4561 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
     4621 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
     4681 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
     4741 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
     4801 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
     4861 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
     4921 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
     4981 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
     5041 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
     5101 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
     5161 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag
     5221 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
     5281 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
     5341 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
     5401 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
     5461 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     5521 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     5581 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     5641 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
     5701 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
     5761 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
     5821 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag
     5881 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
     5941 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
     6001 ttcagcaaag agtctatcag gcccaagagg aacagcgata agctgatcgc cagaaagaag
     6061 gactgggacc ctaagaagta cggcggcttc gtcagcccca ccgtggccta ttctgtgctg
     6121 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
     6181 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
     6241 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
     6301 gagctggaaa acggccggaa gagaatgctg gcctctgcca gattcctgca gaagggaaac
     6361 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
     6421 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
     6481 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
     6541 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
     6601 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tagggccttc
     6661 aagtactttg acaccaccat cgaccggaag gtgtacagga gcaccaaaga ggtgctggac
     6721 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
     6781 ctgggaggtg actctggagg atctagcgga ggatcctctg gcagcgagac accaggaaca
     6841 agcgagtcag caacaccaga gagcagcggc ggcagcagcg gcggcagcat ggccggagca
     6901 caggattttg tcccacatac tgccgatctg gctgagctgg ctgccgccgc tggagagtgt
     6961 agaggatgcg ggctgtaccg ggatgccaca caggccgtgt tcggagcagg cggcaggagc
     7021 gcccgcatca tgatgatcgg cgagcagcca ggcgataagg aggacctggc cggcctgcca
     7081 tttgtgggac cagcaggccg gctgctggac agagccctgg aggccgccga catcgatagg
     7141 gacgccctgt acgtgaccaa cgccgtgaag cacttcaagt ttacacgcgc cgcaggaggc
     7201 aagaggagaa tccacaagac cccctctcgg acagaggtgg tggcctgcag accttggctg
     7261 atcgccgaga tgaccagcgt ggagccagat gtggtggtgc tgctgggagc aacagcagca
     7321 aaggccctgc tgggcaatga cttcagggtg acccagcaca ggggagaggt gctgcacgtg
     7381 gacgatgtgc caggcgatcc tgccctggtg gcaaccgtgc accctagctc cctgctgaga
     7441 ggcccaaagg aggagcggga atccgccttc gctgggctgg tggacgacct gagagtggct
     7501 gccgatgtga gacctaaaag aaccgccgac ggcagcgaat tcgagcccaa gaagaagagg
     7561 aaagtctaac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
     7621 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     7681 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     7741 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     7801 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     7861 gaaccagctg gggctcgata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7921 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7981 gcataaagtg taaagcctag ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8041 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8101 aacgcgcggg aagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8161 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8221 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8281 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8341 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8401 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8461 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8521 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8581 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8641 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8701 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8761 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8821 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8881 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgaca
     8941 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9001 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9061 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9121 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9181 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9241 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9301 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9361 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9421 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9481 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9541 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9601 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9661 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9721 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9781 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9841 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9901 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9961 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10021 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    10081 cgggagatcg atctcccgat cccctagggt cgactctcag tacaatctgc tctgatgccg
    10141 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
    10201 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
    10261 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
    10321 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    10381 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    10441 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    10501 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
//
