LOCUS       sequence-324349-        8619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor plasmid for integrating pEF promoter-driven dual surface 
            marker/fluorescent reporter at AAVS1 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8619)
  AUTHORS   Tycko J, DelRosso N, Hess GT, Aradhana, Banerjee A, Mukund A, Van 
            MV, Ego BK, Yao D, Spees K, Suzuki P, Marinov GK, Kundaje A, Bassik 
            MC, Bintu L
  TITLE     High-Throughput Discovery and Characterization of Human 
            Transcriptional Effectors.
  JOURNAL   Cell. 2020 Dec 9. pii: S0092-8674(20)31541-5. doi: 
            10.1016/j.cell.2020.11.024.
  PUBMED    33326746
REFERENCE   2  (bases 1 to 8619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Dec 9. pii: S0092-8674(20)31541-5. doi: 10.1016/j.cell.2020.11.024."
COMMENT     Sequence Label: pJT039
            AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_...
FEATURES             Location/Qualifiers
     source          1..8619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2..23
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(38..43,44..61,62..68)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    76..92
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     81..103
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     100..116
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     100..116
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        137..155
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    190..993
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    1000..1025
                     /label=SA
                     /note="splice acceptor site"
     CDS             1049..1102
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1112..1711
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1112..1131)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1608..1628
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1743..1760)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1749..1973
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2046..2064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2082..2100
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2118..2136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2154..2172
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2190..2208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2226..2244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2262..2280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2298..2316
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2334..2352
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          2597..3535
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3492..3512
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        3568..3586
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(3596..3607)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     regulatory      3617..3626
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     3623..3682
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             4454..4483
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4487..4633
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             4649..4702
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      4709..4718
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4715..4717,4718..4720,4721..5431)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4760..4781)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5021..5040)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5368..5389
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5432..5452
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5463..5584
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5500..5519)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5554..5573
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    5650..6486
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     CDS             complement(join(6761..7552,7553..7621))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7384..7403
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7745..8333
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8234..8253
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8487..8504
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
       61 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
      121 attacgccaa gctcagaatt aaccctcact aaagggacta gtcctgcagg tttaaacgaa
      181 ttcgcccttt gctttctctg accagcattc tctcccctgg gcctgtgccg ctttctgtct
      241 gcagcttgtg gcctgggtca cctctacggc tggcccagat ccttccctgc cgcctccttc
      301 aggttccgtc ttcctccact ccctcttccc cttgctctct gctgtgttgc tgcccaagga
      361 tgctctttcc ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc tgagcggatc
      421 ctccccgtgt ctgggtcctc tccgggcatc tctcctccct cacccaaccc catgccgtct
      481 tcactcgctg ggttcccttt tccttctcct tctggggcct gtgccatctc tcgtttctta
      541 ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc tccccttctt gtaggcctgc
      601 atcatcaccg tttttctgga caaccccaaa gtaccccgtc tccctggctt tagccacctc
      661 tccatcctct tgctttcttt gcctggacac cccgttctcc tgtggattcg ggtcacctct
      721 cactcctttc atttgggcag ctcccctacc ccccttacct ctctagtctg tgctagctct
      781 tccagccccc tgtcatggca tcttccaggg gtccgagagc tcagctagtc ttcttcctcc
      841 aacccgggcc cctatgtcca cttcaggaca gcatgtttgc tgcctccagg gatcctgtgt
      901 ccccgagctg ggaccacctt atattcccag ggccggttaa tgtggctctg gttctgggta
      961 cttttatctg tcccctccac cccacagtgg ggcaagcttc tgacctcttc tcttcctccc
     1021 acagggcctc gagagatctg gcagcggaga gggcagagga agtcttctaa catgcggtga
     1081 cgtggaggag aatcccggcc ctaggctcga gatgaccgag tacaagccca cggtgcgcct
     1141 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     1201 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     1261 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     1321 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     1381 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     1441 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     1501 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     1561 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     1621 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     1681 ctggtgcatg acccgcaagc ccggtgcctg atctagaggg cccgtttaaa cccgctgatc
     1741 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     1801 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     1861 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     1921 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     1981 ggcggaaaga accagctggg ggctagcaaa gcctaggaac agctgcatat agcactgcag
     2041 tttactccct atcagtgata gagagtcaaa aggccatgcc ctccctatca gtgatagaga
     2101 gagcagactc aaggtggtcc ctatcagtga tagagagatt gagcaagagg gcctccctat
     2161 cagtgataga gaatagcact gcagtttact ccctatcagt gatagagagt caaaaggcca
     2221 tgccctccct atcagtgata gagagagcag actcaaggtg gtccctatca gtgatagaga
     2281 gattgagcaa gagggcctcc ctatcagtga tagagattga tccagtcagg gcctccctat
     2341 cagtgataga gaagctatgt gcaggtaggg ctgtacagtg ggaatgcatg agcgcacatc
     2401 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     2461 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     2521 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     2581 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     2641 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     2701 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     2761 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     2821 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     2881 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
     2941 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     3001 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     3061 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     3121 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     3181 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     3241 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     3301 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     3361 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     3421 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     3481 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     3541 gtgagctagc gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctgga
     3601 gctcccaccg cggtgggcca ccatggagac agacacactc ctgctatggg tactgctgct
     3661 ctgggttcca ggttccactg gtgacgctag cgagacgggc ggatcaggcg gtcgtctcgg
     3721 atccgacaaa actcacacat gcccaccgtg cccagcacct gaactcctgg ggggaccgtc
     3781 agtcttcctc ttccccccaa aacccaagga caccctcatg atctcccgga cccctgaggt
     3841 cacatgcgtg gtggtggacg tgagccacga agaccctgag gtcaagttca actggtacgt
     3901 ggacggcgtg gaggtgcata atgccaagac aaagccgcgg gaggagcagt acaacagcac
     3961 gtaccgtgtg gtcagcgtcc tcaccgtcct gcaccaggac tggctgaatg gcaaggagta
     4021 caagtgcaag gtctccaaca aagccctccc agcccccatc gagaaaacca tctccaaagc
     4081 caaagggcag ccccgagaac cacaggtcta caccctgccc ccatcccggg aggagatgac
     4141 caagaaccag gtcagcctga cctgcctggt caaaggcttc tatcccagcg acatcgccgt
     4201 ggagtgggag agcaatgggc agccggagaa caactacaag accacgcctc ccgtgctgga
     4261 ctccgacggc tccttcttcc tctacagcaa gctcaccgtg gacaagagca ggtggcagca
     4321 ggggaacgtc ttctcatgct ccgtgatgca cgaggctctg cacaaccact acacgcagaa
     4381 gagcctctcc ctgtctccgg gtaaaggagg aggtggaagc ggaggaggag gaagcggagg
     4441 aggaggtagc ggagaacaaa aactcatctc agaagaggat ctgaatgctg tgggccagga
     4501 cacgcaggag gtcatcgtgg tgccacactc cttgcccttt aaggtggtgg tgatctcagc
     4561 catcctggcc ctggtggtgc tcaccatcat ctcccttatc atcctcatca tgctttggca
     4621 gaagaagcca cgttgtacag gcagtggaga gggcagagga agtctgctaa catgcggtga
     4681 cgtcgaggag aatcctggcc caccggtcgc caccatggtg agcaagggcg aggagctgtt
     4741 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     4801 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     4861 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct
     4921 gatgtgcttc gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     4981 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     5041 ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat
     5101 cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca
     5161 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     5221 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     5281 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag
     5341 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     5401 gatcactctc ggcatggacg agctgtacaa gccaaaaaag aagagaaagg tatagttaac
     5461 taaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5521 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5581 cttatcatgt ctgcatatgt aaattgtaag cgtctcggcc tctagagtcg agatgacatt
     5641 gattattgaa ctagggacag gattggtgac agaaaagccc catccttagg cctcctcctt
     5701 cctagtctcc tgatattggg tctaaccccc acctcctgtt aggcagattc cttatctggt
     5761 gacacacccc catttcctgg agccatctct ctccttgcca gaacctctaa ggtttgctta
     5821 cgatggagcc agagaggatc ctgggaggga gagcttggca gggggtggga gggaaggggg
     5881 ggatgcgtga cctgcccggt tctcagtggc caccctgcgc taccctctcc cagaacctga
     5941 gctgctctga cgcggctgtc tggtgcgttt cactgatcct ggtgctgcag cttccttaca
     6001 cttcccaaga ggagaagcag tttggaaaaa caaaatcaga ataagttggt cctgagttct
     6061 aactttggct cttcaccttt ctagtcccca atttatattg ttcctccgtg cgtcagtttt
     6121 acctgtgaga taaggccagt agccagcccc gtcctggcag ggctgtggtg aggagggggg
     6181 tgtccgtgtg gaaaactccc tttgtgagaa tggtgcgtcc taggtgttca ccaggtcgtg
     6241 gccgcctcta ctccctttct ctttctccat ccttctttcc ttaaagagtc cccagtgcta
     6301 tctgggacat attcctccgc ccagagcagg gtcccgcttc cctaaggccc tgctctgggc
     6361 ttctgggttt gagtccttgg caagcccagg agaggcgctc aggcttccct gtcccccttc
     6421 ctcgtccacc atctcatgcc cctggctctc ctgccccttc cctacagggg ttcctggctc
     6481 tgctcttcgc cttcttgacg agttcttctg aattattaac gcttacaatt tcctgatgcg
     6541 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atcaggtggc acttttcggg
     6601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     6661 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     6721 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     6781 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     6841 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     6901 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     6961 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7021 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7081 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7141 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7201 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7261 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gaatactcaa
     7321 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7381 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7441 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7501 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     7561 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     7621 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgaccaaaa
     7681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     7741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     7801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     7861 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     7921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     7981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     8041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     8101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     8161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     8221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     8281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     8341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     8401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     8461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     8521 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     8581 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaa
//
