LOCUS       sequence-32440-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBMPGAL4LWL
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(522..538)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(522..538)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    complement(556..589)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(774..792)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 ccccaccaaa cccaaaaaaa gagtaaaatg aatcgtagat actgaaaaac cccgcaagtt
       61 cacttcaact gtgcatcgtg caccatctca atttctttca tttatacatc gttttgcctt
      121 cttttatgta actatactcc tctaagtttc aatcttggcc atgtaacctc tgatctatag
      181 aattttttaa atgactagaa ttaatgccca tctttttttt ggacctaaat tcttcatgaa
      241 aatatattac gagggcttat tcagaagctt atcgataccg tcgactaaag ccaaatagaa
      301 aattattcag ttcctggctt aagtttttaa aagtgatatt atttatttgg ttgtaaccaa
      361 ccaaaagaat gtaaataact aatacataat tatgttagtt ttaagttagc aacaaattga
      421 ttttagctat attagctact tggttaataa atagaatata tttatttaaa gataattgcg
      481 tttttattgt cagggagtga gtttgcttaa aaactcgttt agatccacta gttctagagc
      541 ggccggccgc tctagataac ttcgtatagc atacattata cgaagttata gcttctgatg
      601 gaattagaac ttggcaaaac aatactgaga atgaagtgta tgtggaacag aggctgctga
      661 tctcgttctt caggctatga aactgacaca tttggaaacc acagtactta gaaccacaaa
      721 gtgggaatca agagaaaaac aatgatccca cgagagatcg aattctagta tgtatgtaag
      781 ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac attttttttt tttactgcac
      841 tggatatcat tgaacttatc tgatcagttt taaatttact tc
//
