LOCUS       sequence-32441-e         671 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Gohl DM, Silies MA, Gao XJ, Bhalerao S, Luongo FJ, Lin CC, Potter 
            CJ, Clandinin TR
  TITLE     A versatile in vivo system for directed dissection of gene 
            expression patterns.
  JOURNAL   Nat Methods. 2011 Mar;8(3):231-7. doi: 10.1038/nmeth.1561.
  PUBMED    21473015
REFERENCE   2  (bases 1 to 671)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-03"; volume: "8"; issue: 
            "3"; pages: "231-7"
COMMENT     Sequence Label: pBMPGAL4LWL
FEATURES             Location/Qualifiers
     source          1..671
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..127
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     317..333
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     318..334
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    465..564
                     /label=phage phi-C31 attP
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attachment site of phage phi-C31"
ORIGIN
        1 gactcactat agggcgaatt gggtaccggg ccccccctcg acgatgtagg tcacggtctc
       61 gaagccgcgg tgcgggtgcc agggcgtgcc cttgggctcc ccgggcgcgt actccacctc
      121 acccatctgg tccatcatga tgaacgggtc gaggtggcgg tagttgatcc cggcgaacgc
      181 gcggcgcacc gggaagccct cgccctcgaa accgctgggc gcggtggtca cggtgagcac
      241 gggacgtgcg acggcgtcgg cgggtgcgga tacgcggggc agcgtcagcg ggttctcgac
      301 ggtcacggcg ggcatgtcga ggtcgacggt atcgataagc ttgcggccgc ggcgcgccag
      361 atcttaatta agctagttgc aggaattcgg cttcgactag tactgacgga cacaccgaag
      421 ccccggcggc aaccctcagc ggatgccccg gggcttcacg ttttcccagg tcagaagcgg
      481 ttttcgggag tagtgcccca actggggtaa cctttgagtt ctctcagttg ggggcgtagg
      541 gtcgccgaca tgacacaagg ggttgtgacc ggggtggaca cgtacgcggg tgcttacgac
      601 cgtcagtcgc gcgagcgcga ctagtacaag ccgaattcga tatcaagctt accgaagtat
      661 acacttaaat t
//
