LOCUS       sequence-32446-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Marumoto T, Tashiro A, Friedmann-Morvinski D, Scadeng M, Soda Y, 
            Gage FH, Verma IM
  TITLE     Development of a novel mouse glioma model using lentiviral vectors.
  JOURNAL   Nat Med. 2009 Jan . 15(1):110-6.
  PUBMED    19122659
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med. 
            2009 Jan . 15(1):110-6."
COMMENT     Sequence Label: pTomo-Ras
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..53
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             71..94
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(95..124,125..127,128..661)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="TEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVI
                     DGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKD
                     SDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIR
                     QHKLRKLNPPDESGPGCMSCKCVLS"
ORIGIN
        1 gccactccac cggcgcctaa ataacttcgt ataatgtatg ctatacgaag ttaatggatc
       61 caccgccatg gactacaagg acgatgatga caagacagaa tacaagctgg tggtggtggg
      121 cgccgtcggt gtgggcaaga gtgcgctgac catccagctg atccagaacc attttgtgga
      181 cgaatacgac cccactatag aggattccta ccggaagcag gtggtcattg atggggagac
      241 gtgcctgttg gacatcctgg ataccgccgg ccaggaggag tacagcgcca tgcgggacca
      301 gtacatgcgc accggggagg gcttcctgtg tgtgtttgcc atcaacaaca ccaagtcttt
      361 tgaggacatc caccagtaca gggagcagat caaacgggtg aaggactcgg atgacgtgcc
      421 catggtgctg gtggggaaca agtgtgacct ggctgcacgc actgtggaat ctcggcaggc
      481 tcaggacctc gcccgaagct acggcatccc ctacatcgag acctcggcca agacccggca
      541 gggagtggag gatgccttct acacgttggt gcgtgagatc cggcagcaca agctgcggaa
      601 gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg tgctctcctg
      661 aggatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
      721 ggccggtgtg cgtttgtcta tatgttattt tccaccata
//
