LOCUS       sequence-324484-       12614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas12a oscillator, reverse crRNAs + PA4-mVenus. (Integration of 
            511; pOSIP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12614)
  AUTHORS   Kuo J, Yuan R, Sanchez C, Paulsson J, Silver PA
  TITLE     Toward a translationally independent RNA-based synthetic oscillator 
            using deactivated CRISPR-Cas.
  JOURNAL   Nucleic Acids Res. 2020 Aug 20;48(14):8165-8177. doi: 
            10.1093/nar/gkaa557.
  PUBMED    32609820
REFERENCE   2  (bases 1 to 12614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-08-20- 20"; volume: "48"; issue: 
            "14"; pages: "8165-8177"
COMMENT     Sequence Label: pJK528
FEATURES             Location/Qualifiers
     source          1..12614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        133..182
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     RBS             212..223
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             232..945
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             232..945
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(262..290)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     885..905
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      1006..1092
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(1105..1199)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1220..1879)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1794..1813
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1880..1982)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             complement(2012..2638)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     2543..2562
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        2654..2709
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    2690..2708
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             2727..2738
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(6843..6877)
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     terminator      7351..7394
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      7516..7543
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(7592..7623)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     protein_bind    7700..7733
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(7740..8453)
                     /codon_start=1
                     /gene="cIts"
                     /product="temperature-sensitive variant of the phage lambda
                     repressor"
                     /label=lambda repressor (ts)
                     /note="thermosensitivity is conferred by the A67T mutation"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLTKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPKLRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     RBS             8542..8550
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             8562..8584
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8592..9602
                     /codon_start=1
                     /gene="int"
                     /product="integrase from phage 186"
                     /label=phage 186 integrase
                     /translation="MTVRKNPAGGWICELYPNGAKGKRIRKKFATKGEALAFEQYTVQN
                     PWQEEKEDRRTLKGLVDSWYSAHGITLKDGLKRQLAMHHAFECMGEPLARDFDAQMFSR
                     YREKRLKGEYARSNRVKEVSPRTLNLELAYFRAVFNELNRLGEWKGENPLKNMRPFRTE
                     EMEMTWLTHDQISQLLGECNRHDHPDLETVVRICLATGARWSEAESLRKSQLAKYKITY
                     TNTKGRKNRTVPISKELYESLPDDKKGRLFSDCYGAFRSALERTGIELPAGQLTHVLRH
                     TFASHFMMNGGNILVLQRVLGHTDIKMTMRYAHFAPDHLEDAVKLNPLVHITNSK"
     primer_bind     complement(9765..9784)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             9776..10591
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(9849..9868)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      10713..11101
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    11112..11159
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    11172..11386
                     /gene="attP"
                     /label=phage 186 attP
                     /bound_moiety="phage 186 integrase"
                     /note="attachment site of phage 186"
     terminator      complement(11503..11749)
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     primer_bind     complement(11840..11862)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     terminator      12072..12158
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      12255..12341
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      12438..12524
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagggctta ctaaaagcca gataacagta
       61 tgcgtatttg cgcgcttggc ctagtatgcg tctcctttac acctccacaa ctagctggta
      121 ttatgctagc agctgtcacc ggatgtgctt tccggtctga tgagtccgtg aggacgaaac
      181 agcctctaca aataattttg tttaacttta caaagaggag aaaggtaccg catgagtaaa
      241 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat
      301 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc
      361 cttaaattga tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact
      421 ttgggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt
      481 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac
      541 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc
      601 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac
      661 aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagcg
      721 aacttcaaaa ttagacacaa cattgaagat ggaggtgttc aactagcaga ccattatcaa
      781 caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta cctgtcctac
      841 caatctaagc tttcgaaaga tcccaacgaa aagagagacc acatggtcct tcttgagttt
      901 gtaacagctg ctgggattac acatggcatg gatgaactat acaaataagc ttgatatcga
      961 attcctgcag cccgggggat cccattgtac gcgtgctaga ggcatcaaat aaaacgaaag
     1021 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
     1081 agtaggacaa attctagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1141 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1201 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     1261 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1321 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1381 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1441 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1501 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1561 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1621 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1681 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1741 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1801 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1861 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1921 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
     1981 caacgtctca ttttcgccag atatcgacgt cttaagaccc actttcacat ttaagttgtt
     2041 tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt
     2101 gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt
     2161 ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat
     2221 gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa
     2281 aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta
     2341 cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta
     2401 aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca
     2461 tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag
     2521 gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct
     2581 cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta gacatcatta
     2641 attcctaatt tttgttgaca ctctatcgtt gatagagtta ttttaccact ccctatcagt
     2701 gatagagaaa agaattcaaa agatctaaag aggagaaagg atctatgtca atttatcaag
     2761 aatttgttaa taaatatagt ttaagtaaaa ctctaagatt tgagttaatc ccacagggta
     2821 aaacacttga aaacataaaa gcaagaggtt tgattttaga tgatgagaaa agagctaaag
     2881 actacaaaaa ggctaaacaa ataattgata aatatcatca gttttttata gaggagatat
     2941 taagttcggt ttgtattagc gaagatttat tacaaaacta ttctgatgtt tattttaaac
     3001 ttaaaaagag tgatgatgat aatctacaaa aagattttaa aagtgcaaaa gatacgataa
     3061 agaaacaaat atctgaatat ataaaggact cagagaaatt taagaatttg tttaatcaaa
     3121 accttatcga tgctaaaaaa gggcaagagt cagatttaat tctatggcta aagcaatcta
     3181 aggataatgg tatagaacta tttaaagcca atagtgatat cacagatata gatgaggcgt
     3241 tagaaataat caaatctttt aaaggttgga caacttattt taagggtttt catgaaaata
     3301 gaaaaaatgt ttatagtagc aatgatattc ctacatctat tatttatagg atagtagatg
     3361 ataatttgcc taaatttcta gaaaataaag ctaagtatga gagtttaaaa gacaaagctc
     3421 cagaagctat aaactatgaa caaattaaaa aagatttggc agaagagcta acctttgata
     3481 ttgactacaa aacatctgaa gttaatcaaa gagttttttc acttgatgaa gtttttgaga
     3541 tagcaaactt taataattat ctaaatcaaa gtggtattac taaatttaat actattattg
     3601 gtggtaaatt tgtaaatggt gaaaatacaa agagaaaagg tataaatgaa tatataaatc
     3661 tatactcaca gcaaataaat gataaaacac tcaaaaaata taaaatgagt gttttattta
     3721 agcaaatttt aagtgataca gaatctaaat cttttgtaat tgataagtta gaagatgata
     3781 gtgatgtagt tacaacgatg caaagttttt atgagcaaat agcagctttt aaaacagtag
     3841 aagaaaaatc tattaaagaa acactatctt tattatttga tgatttaaaa gctcaaaaac
     3901 ttgatttgag taaaatttat tttaaaaatg ataaatctct tactgatcta tcacaacaag
     3961 tttttgatga ttatagtgtt attggtacag cggtactaga atatataact caacaaatag
     4021 cacctaaaaa tcttgataac cctagtaaga aagagcaaga attaatagcc aaaaaaactg
     4081 aaaaagcaaa atacttatct ctagaaacta taaagcttgc cttagaagaa tttaataagc
     4141 atagagatat agataaacag tgtaggtttg aagaaatact tgcaaacttt gcggctattc
     4201 cgatgatatt tgatgaaata gctcaaaaca aagacaattt ggcacagata tctatcaaat
     4261 atcaaaatca aggtaaaaaa gacctacttc aagctagtgc ggaagatgat gttaaagcta
     4321 tcaaggatct tttagatcaa actaataatc tcttacataa actaaaaata tttcatatta
     4381 gtcagtcaga agataaggca aatattttag acaaggatga gcatttttat ctagtatttg
     4441 aggagtgcta ctttgagcta gcgaatatag tgcctcttta taacaaaatt agaaactata
     4501 taactcaaaa gccatatagt gatgagaaat ttaagctcaa ttttgagaac tcgactttgg
     4561 ctaatggttg ggataaaaat aaagagcctg acaatacggc aattttattt atcaaagatg
     4621 ataaatatta tctgggtgtg atgaataaga aaaataacaa aatatttgat gataaagcta
     4681 tcaaagaaaa taaaggcgag ggttataaaa aaattgttta taaactttta cctggcgcaa
     4741 ataaaatgtt acctaaggtt ttcttttctg ctaaatctat aaaattttat aatcctagtg
     4801 aagatatact tagaataaga aatcattcca cacatacaaa aaatggtagt cctcaaaaag
     4861 gatatgaaaa atttgagttt aatattgaag attgccgaaa atttatagat ttttataaac
     4921 agtctataag taagcatccg gagtggaaag attttggatt tagattttct gatactcaaa
     4981 gatataattc tatagatgaa ttttatagag aagttgaaaa tcaaggctac aaactaactt
     5041 ttgaaaatat atcagagagc tatattgata gcgtagttaa tcagggtaaa ttgtacctat
     5101 tccaaatcta taataaagat ttttcagctt atagcaaagg gcgaccaaat ctacatactt
     5161 tatattggaa agcgctgttt gatgagagaa atcttcaaga tgtggtttat aagctaaatg
     5221 gtgaggcaga gcttttttat cgtaaacaat caatacctaa aaaaatcact cacccagcta
     5281 aagaggcaat agctaataaa aacaaagata atcctaaaaa agagagtgtt tttgaatatg
     5341 atttaatcaa agataaacgc tttactgaag ataagttttt ctttcactgt cctattacaa
     5401 tcaattttaa atctagtgga gctaataagt ttaatgatga aatcaattta ttgctaaaag
     5461 aaaaagcaaa tgatgttcat atattaagta tagctagagg tgaaagacat ttagcttact
     5521 atactttggt agatggtaaa ggcaatatca tcaaacaaga tactttcaac atcattggta
     5581 atgatagaat gaaaacaaac taccatgata agcttgctgc aatagagaaa gatagggatt
     5641 cagctaggaa agactggaaa aagataaata acatcaaaga gatgaaagag ggctatctat
     5701 ctcaggtagt tcatgaaata gctaagctag ttatagagta taatgctatt gtggtttttg
     5761 aggatttaaa ttttggattt aaaagagggc gtttcaaggt agagaagcag gtctatcaaa
     5821 agttagaaaa aatgctaatt gagaaactaa actatctagt tttcaaagat aatgagtttg
     5881 ataaaactgg gggagtgctt agagcttatc agctaacagc accttttgag acttttaaaa
     5941 agatgggtaa acaaacaggt attatctact atgtaccagc tggttttact tcaaaaattt
     6001 gtcctgtaac tggttttgta aatcagttat atcctaagta tgaaagtgtc agcaaatctc
     6061 aagagttctt tagtaagttt gacaagattt gttataacct tgataagggc tattttgagt
     6121 ttagttttga ttataaaaac tttggtgaca aggctgccaa aggcaagtgg actatagcta
     6181 gctttgggag tagattgatt aactttagaa attcagataa aaatcataat tgggatactc
     6241 gagaagttta tccaactaaa gagttggaga aattgctaaa agattattct atcgaatatg
     6301 ggcatggcga atgtatcaaa gcagctattt gcggtgagag cgacaaaaag ttttttgcta
     6361 agctaactag tgtcctaaat actatcttac aaatgcgtaa ctcaaaaaca ggtactgagt
     6421 tagattatct aatttcacca gtagcagatg taaatggcaa tttctttgat tcgcgacagg
     6481 cgccaaaaaa tatgcctcaa gatgctgatg ccaatggtgc ttatcatatt gggctaaaag
     6541 gtctgatgct actaggtagg atcaaaaata atcaagaggg caaaaaactc aatttggtta
     6601 tcaaaaatga agagtatttt gagttcgtgc agaataggaa taactaattg agaagagaaa
     6661 agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga ccggcggtct
     6721 taagtttttt ggctgaatta attaaggacc aaaacgaaaa aagacgcttt tcagcgtctt
     6781 attgttcgtc tttggtaccg agaccagcta gttgtggagg tgatctacaa cagtagaaat
     6841 tagctagcat aatacctagg actgagctag gtgtaaaatc ctatcttaaa caaaattatt
     6901 tgtagaggtc ataagtttcg tcgcatttca gcgactcatc agtgggctta gcccagactt
     6961 atgactggat ccggaccaaa acgaaaaaag acgctcgaaa gcgtctcttt tctggaattt
     7021 ggtaccgaga cccgtgtgac ccgttggtga tctacaacag tagaaattag ctagcataat
     7081 accagctagt tgtggaggtg taaaaagctt aaacaaaatt atttgtagag gactgtttcg
     7141 gcccttttgg gccatcgtca ggtcggatac acatccggcg acagtctgga ccaaaacgaa
     7201 aaaaggcccc cctttcggga ggcctctttt ctggaatttg gtaccgagac ctaggactga
     7261 gctaggtgat ctacaacagt agaaattagc tagcataata cccgtgtgac ccgttggtgt
     7321 aaaatgattt ctgagatctt tagaattcca gaaatcatcc ttagcgaaag ctaaggattt
     7381 tttttatctg aaattctgcc tcgtgatacg cctattaatt aagtacgggt tttgctgccc
     7441 gcaaacgggc tgttctggtg tgcatgcctc gagatgcatg gcgcctaacc taaactgaca
     7501 ggcatcaaat taagcagaag gccatcctga cggatggcct ttttgcgttt cgaacaattg
     7561 aaaaaacctc gcgccttacc tgttgagtaa tagtcaaaag cctccggtcg gaggcttttg
     7621 actttctgct tactgaattt cggtggtgcc gttaattaac cggtgggccc tcatgataat
     7681 aatggtttct tagacgtccg aagttcctat tctctagaaa gtataggaac ttccctaggt
     7741 cagccaaacg tctcttcagg ccactgacta gcgataactt tccccacaac ggaacaactc
     7801 tcattgcatg ggatcattgg gtactgtggg tttagtggtt gtaaaaacac ctgaccgcta
     7861 tccctgatca gtttcttgaa ggtaaactca tcacccccaa gtctggctat gcagaaatca
     7921 cctggctcaa cagcctgctc agggtcaacg agaattaaca ttccgtcagg aaagcttggc
     7981 ttggagcctg ttggtgcggt catggaatta ccttcaacct caagccagaa tgcagaatca
     8041 ctggcttttt tggttgtgct tacccatctc tccgcatcac ctttggtaaa ggttctaagc
     8101 ttaggtgaga acatccctgc ctgaacatga gaaaaaacag ggtactcata ctcacttcta
     8161 agtgacggct gcatactaac cgcttcatac atctcgtaga tttctctggc gattgaaggg
     8221 ctaaattctt caacgctaac tttgagaatt tttgtaagca atgcggcgtt ataagcattt
     8281 aatgcattga tgccattaaa taaagcacca acgcctgact gccccatccc catcttgtct
     8341 gcgacagatt cctgggataa gccaagttca tttttctttt tttcataaat tgctttaagg
     8401 cgacgtgcgt cctcaagctg ctcttgtgtt aatggtttct tttttgtgct catacgttaa
     8461 atctatcacc gcaagggata aatatctaac accgtgcgtg ttgactattt tacctctggc
     8521 ggtgataatg gttgcatgta ctaaggaggt tgtatacgcg ttttgtttaa ctttaagaag
     8581 gagatataca tatgaccgtc cgtaaaaatc cggctggcgg ttggatttgt gaactctacc
     8641 caaacggtgc aaaaggcaaa cgtatcagaa agaaattcgc tactaaaggc gaggctctgg
     8701 cgtttgagca gtacaccgtt caaaacccgt ggcaggaaga aaaggaagac aggcgcacgt
     8761 taaaagggct ggttgattca tggtatagcg ctcatggcat tacactgaaa gatggtttga
     8821 aacgccagtt agccatgcac catgcttttg agtgtatggg cgaaccactc gcacgcgatt
     8881 tcgatgcgca gatgttttcc cgctaccgag aaaaacggtt aaaaggtgag tatgcccgtt
     8941 caaacagagt gaaagaggta tcgcctcgca cgcttaatct tgagctggcc tacttccggg
     9001 cggtgttcaa tgagctaaac cgcctcggag aatggaaggg tgaaaaccca ctaaaaaata
     9061 tgcgcccatt ccgcacagaa gaaatggaaa tgacttggct aactcacgac caaatttcgc
     9121 aactgctcgg agagtgtaac cgacatgacc accctgattt agaaaccgtg gtaagaatct
     9181 gtctcgcaac tggcgcacgg tggtctgagg ccgagagtct gagaaaaagc caactcgcga
     9241 aatacaaaat cacatacacc aacacgaaag gtagaaaaaa tcgcaccgtc ccaatcagca
     9301 aagagctcta tgagtctctg cctgatgata aaaagggccg gttgttcagt gattgttatg
     9361 gcgcgttccg gtcagctttg gaaagaacag gtatcgaact accggcagga caacttaccc
     9421 acgttttacg tcacaccttc gctagtcact ttatgatgaa tggcggtaat attttggtgt
     9481 tgcagcgcgt actcggccat acagatataa aaatgaccat gcgatatgcg cactttgcac
     9541 ccgaccattt agaggatgca gttaaactaa atcctttagt tcacattact aacagcaaat
     9601 aagttatgga ggttgtatgt cagttcggct agcccatggg tatggacagt tttccctttg
     9661 atatgtaacg cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat
     9721 atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag
     9781 ccatattcaa cgggaaacgt cttgctcccg tccgcgctta aactccaaca tggacgctga
     9841 tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg
     9901 cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc
     9961 caatgatgtt acagatgaga tggtccgtct caactggctg acggagttta tgcctctccc
    10021 gaccatcaag cattttatcc gtactcctga tgatgcgtgg ttactcacca ccgcgattcc
    10081 tgggaaaaca gccttccagg tattagaaga atatcctgat tcaggtgaaa atattgttga
    10141 tgcgctggcc gtgttcctgc gccggttaca ttcgattcct gtttgtaatt gtccttttaa
    10201 cagcgatcgt gtatttcgtc ttgctcaggc gcaatcacgc atgaataacg gtttggttga
    10261 tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat
    10321 gcacaagctc ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga
    10381 taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gggtcggaat
    10441 cgcagaccgt taccaggacc ttgccattct ttggaactgc ctcggtgagt tttctccttc
    10501 attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca
    10561 gtttcatttg atgctcgatg agtttttcta ataatactag cagaaatcat ccttagcgaa
    10621 agctaaggat tttttttatc tgattaccgc ctttgagtga gcgtcgacct agtgcggccg
    10681 caagatccgg ccacgatgcg tccggcgtag aggatctgaa gatcagcagt tcaacctgtt
    10741 gatagtacgt actaagctct catgtttcac gtactaagct ctcatgttta acgtactaag
    10801 ctctcatgtt taacgaacta aaccctcatg gctaacgtac taagctctca tggctaacgt
    10861 actaagctct catgtttcac gtactaagct ctcatgtttg aacaataaaa ttaatataaa
    10921 tcagcaactt aaatagcctc taaggtttta agttttataa gaaaaaaaag aatatataag
    10981 gcttttaaag cttttaaggt ttaacggttg tggacaacaa gccagggatg taacgcactg
    11041 agaagccctt agagcctctc aaagcaattt tgagtgacac aggaacactt aacggctgac
    11101 atgggaatta ggaagttcct attccgaagt tcctattctc tagaaagtat aggaacttcc
    11161 atatgccatg ggcgatggtt ctgagtaaca gataatagaa tggcgataaa gtggcggtag
    11221 aaatggcgaa taataggtaa ttattggcaa acaatggcaa tctatgtcaa tgataaataa
    11281 cgcatactat tgattttcgg ttgttcttgt aggaactcat aatcgcttgg tcgctggttc
    11341 aagtccagca ggggccacca aatttaagta gtaaatacat acagttaggc cactctctcg
    11401 agtggccttt ttgttgcctc gttttcgagt ggcgatagaa tggcggtgga ttttttaccg
    11461 ccactttcct gaggagtgca tagctagctt cttcgtctgt ttctactggt attggcacaa
    11521 acctgattcc aatttgagca aggctatgtg ccatctcgat actcgttctt aactcaacag
    11581 aagatgcttt gtgcatacag cccctcgttt attatttatc tcctcagcca gccgctgtgc
    11641 tttcagtgga tttcggataa cagaaaggcc gggaaatacc cagcctcgct ttgtaacgga
    11701 gtagagacga aagtgattgc gcctacccgg atattatcgt gaggatgcgt catcgccatt
    11761 aattcactga tcagtgataa gctgtcaaac atgagaattg atccggctgc ctcgcgcgtt
    11821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
    11881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
    11941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatgct gcacatgaca
    12001 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt aattcccaat
    12061 tccccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
    12121 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
    12181 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggaatt
    12241 aattccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
    12301 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga
    12361 acgttgcgaa gcaacggccc ggagggkggc gggcaggacg cccgccataa actgccagga
    12421 attaattccc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
    12481 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
    12541 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
    12601 ggaattgggg atcg
//
