LOCUS       sequence-324532-        2303 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPKK0CM0111
FEATURES             Location/Qualifiers
     source          1..2303
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(523..617)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(638..1297)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1212..1231
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1459..1477
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1481..1524)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    1556..1589
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    1615..1648
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        1665..1711
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1803..1860
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1884..1901)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2055..2303,1..340))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2135..2154)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
       61 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      121 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      181 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      241 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      301 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      361 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      421 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
      481 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
      541 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattactgg
      601 atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc tgccactcat
      661 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
      721 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
      781 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
      841 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
      901 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
      961 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     1021 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgaa
     1081 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     1141 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     1201 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     1261 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     1321 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     1381 aacctcttac gtgcccgatc aactcgagtg ccacctgacg tctaagaaac cattattatc
     1441 atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc
     1501 ttagctttcg ctaaggatga tttctggaat tcggtctcac catggtgcgg tgcgggtgcc
     1561 agggcgtgcc cttgggctcc ccgggcgcgt actccactcc ccgagccggt gcgggtgcca
     1621 gggcgtgccc ttgggctccc cgggcgcgta ctccactccc cttcgcaaga cccttcctct
     1681 atataaggaa gttcatttca tttggagagg actccggtat ttttacaaca attaccacaa
     1741 caaaacaaac aacaaacaac attacaattt actattctag tcgaaaatgc gagaccctgc
     1801 agtccggcaa aaaagggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga
     1861 ttacttcgcg ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     1921 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     1981 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2041 gcgttgctgg cgtttttcca caggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2101 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2161 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2221 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2281 taggtcgttc gctccaagct ggg
//
