LOCUS       sequence-32461-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-150
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(553..577)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             603..644
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(684..703)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    728..776
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 cgaaattaat acgactcact atagggagac ccaagctggc tagttaagct atcaacaagt
       61 ttgtacaaaa aagcaggctc atatgggagt gggtgtgcag tttctgcgac tcagggtggc
      121 gtccccccaa cctgtccctg ccccttcctg ccctctttga tgcggcccca cttcctctgg
      181 caggaacccc cgccctccct ggacctgggt ataaggcagg gactgggccc acggggaggc
      241 agcgtccccg aggcagcagc ggcagcggcg gctcctctcc ccatggccct gtctcccaac
      301 ccttgtacca gtgctgggct cagaccctgg tacaggcctg ggggacaggg acctggggac
      361 cccggcaccg gcaggcccca aggggtgagg tgagcgggca ttgggacctc ccctccctgt
      421 actcccatct ctgctgcggc ttttatgcgt ctctcccctt cgggtcccac atatcctctg
      481 gtgcgctcct gcctcaccgc ccccacccca tgcctgtcgt ccccacctct gtgtgatgcg
      541 caaagtaagc ttacccagct ttcttgtaca aagtggttga tctagagggc ccgcggttcg
      601 aaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggttagtaat
      661 gagtttaaac gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
      721 gctatgacgg caataaaaag acagaataaa acgcacgggt gttgggtcgt ttgttcataa
      781 acgcggggtt cggtcccagg gctgg
//
