LOCUS       sequence-324632-       18194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PP7-tagged reporter driven by the Arabidopsis HsfA2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18194)
  AUTHORS   Alamos S, Reimer A, Niyogi KK, Garcia HG
  TITLE     Quantitative imaging of RNA polymerase II activity in plants reveals
            the single-cell basis of tissue-wide transcriptional dynamics.
  JOURNAL   Nat Plants. 2021 Aug;7(8):1037-1049. doi: 
            10.1038/s41477-021-00976-0. Epub 2021 Aug 9.
  PUBMED    34373604
REFERENCE   2  (bases 1 to 18194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41477-021-00976-0"; journalName: "Nat Plants"; date: 
            "2021-08"; volume: "7"; issue: "8"; pages: "1037-1049"
COMMENT     Sequence Label: AL13Rb-HsfA2
FEATURES             Location/Qualifiers
     source          1..18194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    571..595
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     783..805
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     797..814
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     798..814
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          5819..6007
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     terminator      7436..7683
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             9035..9730
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     9436..9455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(10335..10351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10335..10351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10348..10370)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10359..10375
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10383..10389,10390..10407,10408..10413))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10428..10449
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10640..11316
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11253..11273
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11379..12176
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11436..11455)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12046..12065
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    12233..12407
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12485..12509
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12934..13728
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13815..14403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14304..14323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14557..14574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14589..14729
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14655..14674)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14815..14837
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15073..15267
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15333..16406)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16835..17464)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
       61 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
      121 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
      181 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
      241 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
      301 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
      361 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
      421 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
      481 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
      541 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
      601 tgatagttta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      661 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      721 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      781 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtcga
      841 ctccaattcc tctgtcctcg ataagagctc caataccaga aatgagtaaa aaaccaactc
      901 caatagttcg gattgtactc catagctgtt ctttaaagtg agtcctttct gtggatatcg
      961 tatggatcgg tgcactagca gttccaagga caccatcttt tgttggtttt cctacgtttc
     1021 taaatgcccc aagacctcca aaagtctctt cctcccttgc aacaccagca atacctaaaa
     1081 gaaaaacagc aaaacatttc ccaatattat atagagaaat ggctcagaag ctgctaatac
     1141 aaatatgaaa gcaaggacac aatccaagta tccattcaga ttcaaacaaa cagtctctcc
     1201 ctccttaccc acctctttgc aatgttcgaa ccagctcact ttgatcgagc ctatcaactt
     1261 taaccaatgc cttaatgtat tccgataacg cagaggcatt cgcatgcaaa gaaggttggc
     1321 tttcaaacat tctgataaca gcctcaggat catttcttcg aataagttct ctcagatgtg
     1381 caacctcatt aacttcttct ctatcacgga ctctgcgagc aaagctacca acataactcg
     1441 attgaaacct cgttcgcggt aaagaagctc caccaccacc cacagctata gataaaataa
     1501 aaccaattta catcaaaaac acttctaaaa caacaagaac aactcataaa cggaatcaga
     1561 atttacctcc agtaactccc actctaggaa aagaggagta agctctaaca agtaagcttc
     1621 ttaaactgct caattccctt tcatgactcg aaacctgtca tcggtgaaga gaatcaatac
     1681 agatgaagtt aattactaat cccaaactca aaatgacact agacaacaca tgaaaacatt
     1741 ttgataaaag tgaatacttt tacccatttt gaatcaaaaa aattgaaact ttttatacca
     1801 atttcaaaat taggtgactt gggtagtcaa ataaatcaaa tgacaatatc acagagacaa
     1861 tctagaatcc taaaagacaa cattttgagg gcagagaaga atttgagctt ctagggtttg
     1921 aaaaatatca tctttagctt atgaatcaca aagatcttga taaaacccat cagaaattat
     1981 tatctaatta gatcaaatcc actacagtat caaaccaaag tcgaaacctt tttcgtatta
     2041 aaaattggat aaaggggaaa agagaaaaat gaaacaaaaa ccttggtgat gatgcgcctc
     2101 caagccatca atcaaatctc tccttcacga tatcttaaaa attggtgtta atgtctgata
     2161 aatcgaagtt cctcgatcta tatcggaaac aaaagacttc ttcgttgtgt ttggggaaga
     2221 accgttctaa tcttattccc taaagtctta aaaacactaa attacacgtg agagacctgt
     2281 ttggttatcg ggtgagagaa aaattgtgca gcaggtggag acacgcacga gatatgtagg
     2341 tcgcctctta agtacataaa tacccttgga catacccaat aattcatttt agtaggcttt
     2401 ttctggcggc ccacattaaa aagaaggacc tagaacataa attggcatcg ttagaaatgg
     2461 gcttaagtaa aggcccatat gatatatata taaaaaaaga gattctagat tagtaacgaa
     2521 gtttctggaa cattgtcttg tcttgtcgcc acgtgctcac ataaatgtca aagaagcttc
     2581 aatacagtga aatgatcttg tcttgtctct agaaccttct cttctctccc cttataattt
     2641 catttctctc tcctccacgc ctcaatctct caactcaaaa ctcaacattt tctgaagaaa
     2701 gtcgcaaact ttacccaaaa cccagtttct aattttagca acaaaatcaa aaatatctac
     2761 ttttgtttct cgaaagttac gaaattcata caatctagct tatctctgag cttatggatt
     2821 tgagataaac aacgaaaatg gaagaactga aagtggaaat ggaggaagaa acggtgacgt
     2881 agggatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     2941 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     3001 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     3061 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     3121 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     3181 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     3241 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     3301 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     3361 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     3421 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     3481 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     3541 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     3601 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     3661 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     3721 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     3781 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     3841 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     3901 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     3961 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     4021 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     4081 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     4141 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     4201 tcagatccta cggtacttat tgccaagaaa gcacgagacg atatggcgtc cgtgcctcca
     4261 ggtcgaatct tcaaacgacg agaggatatg gcctccgtcg ctccagtatt ccagggttca
     4321 tcagagtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
     4381 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
     4441 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     4501 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     4561 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
     4621 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
     4681 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     4741 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     4801 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     4861 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     4921 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     4981 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     5041 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     5101 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     5161 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     5221 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     5281 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     5341 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     5401 taaaggccaa gaagggcgga aagatcgccg tgtatggtcc gtcctgtaga aaccccaacc
     5461 cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga
     5521 attggtcagc gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc
     5581 agttttaacg atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat
     5641 cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat
     5701 gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc
     5761 ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtctacgt
     5821 aagtttctgc ttctaccttt gatatatata taataattat cattaattag tagtaatata
     5881 atatttcaaa tatttttttc aaaataaaag aatgtagtat atagcaattg cttttctgta
     5941 gtttataagt gtgtatattt taatttataa cttttctaat atatgaccaa aatttgttga
     6001 tgtgcaggta tcaccgtttg tgtgaacaac gaactgaact ggcagactat cccgccggga
     6061 atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt acttccatga tttctttaac
     6121 tatgccggaa tccatcgcag cgtaatgctc tacaccacgc cgaacacctg ggtggacgat
     6181 atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg cgtctgttga ctggcaggtg
     6241 gtggccaatg gtgatgtcag cgttgaactg cgtgatgcgg atcaacaggt ggttgcaact
     6301 ggacaaggca ctagcgggac tttgcaagtg gtgaatccgc acctctggca accgggtgaa
     6361 ggttatctct atgaactgtg cgtcacagcc aaaagccaga cagagtgtga tatctacccg
     6421 cttcgcgtcg gcatccggtc agtggcagtg aagggcgaac agttcctgat taaccacaaa
     6481 ccgttctact ttactggctt tggtcgtcat gaagatgcgg acttgcgtgg caaaggattc
     6541 gataacgtgc tgatggtgca cgaccacgca ttaatggact ggattggggc caactcctac
     6601 cgtacctcgc attaccctta cgctgaagag atgctcgact gggcagatga acatggcatc
     6661 gtggtgattg atgaaactgc tgctgtcggc tttaacctct ctttaggcat tggtttcgaa
     6721 gcgggcaaca agccgaaaga actgtacagc gaagaggcag tcaacgggga aactcagcaa
     6781 gcgcacttac aggcgattaa agagctgata gcgcgtgaca aaaaccaccc aagcgtggtg
     6841 atgtggagta ttgccaacga accggatacc cgtccgcaag gtgcacggga atatttcgcg
     6901 ccactggcgg aagcaacgcg taaactcgac ccgacgcgtc cgatcacctg cgtcaatgta
     6961 atgttctgcg acgctcacac cgataccatc agcgatctct ttgatgtgct gtgcctgaac
     7021 cgttattacg gatggtatgt ccaaagcggc gatttggaaa cggcagagaa ggtactggaa
     7081 aaagaacttc tggcctggca ggagaaactg catcagccga ttatcatcac cgaatacggc
     7141 gtggatacgt tagccgggct gcactcaatg tacaccgaca tgtggagtga agagtatcag
     7201 tgtgcatggc tggatatgta tcaccgcgtc tttgatcgcg tcagcgccgt cgtcggtgaa
     7261 caggtatgga atttcgccga ttttgcgacc tcgcaaggca tattgcgcgt tggcggtaac
     7321 aagaaaggga tcttcactcg cgaccgcaaa ccgaagtcgg cggcttttct gctgcaaaaa
     7381 cgctggactg gcatgaactt cggtgaaaaa ccgcagcagg gaggcaaaca atgaatatga
     7441 agatgaagat gaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt
     7501 tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat
     7561 ctcattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc
     7621 ttctgcagca tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat
     7681 aagatgggct catagagtaa aacgaggcga gggacctata aacctcactt catcatgcta
     7741 tttcatgatc tattttataa aataaagatg tagaaaaaag taagcgtaat aaccgcaaaa
     7801 caaatgattt aaaacatggc acataatgag gagattaagt tcggtttacg tttattttag
     7861 tactaattgt aacgtgagac tacgtatcgg gaatcgccta ccgggtaccg gctggatctt
     7921 atgacgaccg ttagaaattg tggttgtcga cgagtcagta ataaacggcg tcaaagtggt
     7981 tgcagccggc acacacgagt cgtgtttatc aactcaaagc acaaatactt ttcctcaacc
     8041 taaaaataag gcaattagcc aaaaacaact ttgcgtgtaa acaacgctca atacacgtgt
     8101 cattttatta ttagctattg cttcaccgcc ttagctttct cgtgacctag tcgtcctcgt
     8161 cttttcttct tcttcttcta taaaacaata cccaaagagc tcttcttctt cacaattcag
     8221 atttcaattt ctcaaaatct taaaaacttt ctctcaattc tctctaccgt gatcaaggta
     8281 aatttctgtg ttccttattc tctcaaaatc ttcgattttg ttttcgttcg atcccaattt
     8341 cgtatatgtt ctttggttta gattctgtta atcttagatc gaagacgatt ttctgggttt
     8401 gatcgttaga tatcatctta attctcgatt agggtttcat agatatcatc cgatttgttc
     8461 aaataatttg agttttgtct aataattact cttcgatttg tgatttctat ctagatctgg
     8521 tgttagtttc tagtttgtgc gatcgaattt gtcgattaat ctgagttttt ctgattaaca
     8581 gatggcgaag gcagataaga aaccagcgga gaagaaaccg gcagagaaaa ctccggcagt
     8641 cgaaccagca gcagcggcag agaagaaacc aaaagccgga aagaaactcc ccaaggaacc
     8701 agccgtcgcc ggagacaaga agaagaagag atcaaagaag aacgttgaga catacaagat
     8761 ctacatcttc aaggtgttga agcaagttca tccagacatc ggaatctcaa gcaaagccat
     8821 gggaatcatg aacagtttca tcaacgatat ctttgagaag cttgctggtg agtcttcgaa
     8881 gcttgcgagg tacaacaaga agccgacgat tacttccagg gagattcaga ctgcggttag
     8941 acttgtgttg cccggtgagt tggcgaaaca tgctgtttct gaagggacta aggcggttac
     9001 gaagtttacg agttctgctg gagctggaga attcatggtg agcaagggcg aggcagtgat
     9061 caaggagttc atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga
     9121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     9181 gaccaagggt ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg
     9241 ctccagggcc ttcaccaagc accccgccga catccccgac tactataagc agtccttccc
     9301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac
     9361 ccaggacacc tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa
     9421 cttccctcct gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga
     9481 gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa
     9541 ggacggcggc cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca
     9601 gatgcccggc gcctacaacg tcgaccgcaa gttggacatc acctcccaca acgaggacta
     9661 caccgtggtg gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga
     9721 gctgtacaag taggcttttt gtgatctgat gataagtggt tggttcgtgt ctcatgcact
     9781 tgggaggtga tctatttcac ctggtgtagt ttgtgtttcc gtcagttgga aaaacttatc
     9841 cctatcgatt tcgttttcat tttctgcttt tcttttatgt accttcgttt gggcttgtaa
     9901 cgggcctttg tatttcaact ctcaataata atccaagtgc atgttaaaca atttgtcatc
     9961 tgtttcggct ttgatatact actggtgaag atgggccgta ctactgcatc acaacgaaaa
    10021 ataataataa gatgaaaaac ttgaagtgga aaaaaaaaaa aacttgaatg ttcactacta
    10081 ctcattgacc ataatgttta acatacatag ctcaatagta tttttgtgaa tatggcaaca
    10141 caaacagtcc aaaacaattg tctcttacta taccaaacca agggcgccgc ttgtttgcca
    10201 ctctttgtgt gcaatagtgt gattaccaca tctccacatt caatatattc cctgaattat
    10261 ctgacgattt tgatggctca ctgttttccc aagtctggta ccccacctac atggcagctc
    10321 gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    10381 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    10441 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    10501 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc
    10561 ttgccaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct
    10621 cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg
    10681 gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca
    10741 cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca
    10801 gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa
    10861 ccacgtcttc aaagcaagtg gattgatgtg aacatggtgg agcacgacac tctcgtctac
    10921 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
    10981 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
    11041 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
    11101 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
    11161 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
    11221 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
    11281 taaggaagtt catttcattt ggagaggaca cgctgaaatc accagtctct ctctacaaat
    11341 ctatctctct cgagctttcg cagatctgtc gatcgaccat ggggattgaa caagatggat
    11401 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
    11461 agacaatcgg ctgctctgat gccgccgtgt ttcggctgtc agcgcagggg cgcccggttc
    11521 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact ccaggacgag gcagcgcggc
    11581 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
    11641 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
    11701 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
    11761 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
    11821 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
    11881 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga
    11941 cacatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
    12001 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
    12061 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
    12121 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg
    12181 gactctgggg ttcggatcga tcctctagct agagtcgatc gacaagctcg agtttctcca
    12241 taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat
    12301 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat
    12361 aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcccc cgaattaatt
    12421 cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
    12481 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
    12541 tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga
    12601 gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa
    12661 ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg
    12721 atgacagagc gttgctgcct gtgatcaccg cggtttcaaa atcggctccg tcgatactat
    12781 gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta
    12841 gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt
    12901 taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa
    12961 ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct
    13021 aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg
    13081 tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga
    13141 aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc
    13201 atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa
    13261 aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat
    13321 tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac
    13381 gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc
    13441 gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc
    13501 gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt
    13561 gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg
    13621 gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct
    13681 gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta cctagaatgc
    13741 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    13801 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    13861 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    13921 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
    13981 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    14041 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    14101 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    14161 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    14221 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    14281 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    14341 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    14401 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    14461 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    14521 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    14581 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
    14641 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
    14701 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
    14761 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
    14821 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
    14881 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
    14941 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
    15001 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
    15061 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
    15121 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
    15181 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
    15241 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
    15301 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
    15361 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
    15421 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
    15481 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
    15541 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
    15601 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
    15661 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
    15721 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
    15781 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
    15841 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
    15901 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
    15961 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
    16021 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
    16081 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
    16141 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
    16201 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
    16261 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
    16321 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
    16381 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
    16441 tgcagggccg gcaggcaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
    16501 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
    16561 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
    16621 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
    16681 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
    16741 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
    16801 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
    16861 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
    16921 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
    16981 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
    17041 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
    17101 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
    17161 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
    17221 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
    17281 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
    17341 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
    17401 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
    17461 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
    17521 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
    17581 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
    17641 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
    17701 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
    17761 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
    17821 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
    17881 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
    17941 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
    18001 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
    18061 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
    18121 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
    18181 gcctcctgga tgcg
//
