LOCUS       sequence-32464-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 hsa-mir-214
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    99..123
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(495..519)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             545..586
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(626..645)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    670..718
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 agcgactctc tggctactga gaacccactg gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagtta agctatcaac aagtttgtac aaaaaagcag
      121 gctcatatgt ccatgttagc acatttggaa caaatgtata aatgtacttt ctgaataaag
      181 tcaaaagtct aacttgtttt tagttccata atgttttaat gtttaattat attgtgtatt
      241 tttctccctt tccccttact ctccaaaatc accaaatctg gaaaacaggc tgattgtatc
      301 tgtctatgag caaaggaaac ctgaaggaac caagggcctg gctggacaga gttgtcatgt
      361 gtctgcctgt ctacacttgc tgtgcagaac atccgctcac ctgtacagca ggcacagaca
      421 ggcagtcaca tgacaaccca gcctgaatga caaccagcca ttgaaagaaa gcagccctca
      481 caccatagaa gcttacccag ctttcttgta caaagtggtt gatctagagg gcccgcggtt
      541 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggttagta
      601 atgagtttaa acgggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc
      661 gcgctatgac ggcaataaaa agacagaata aaacgcacgg gtgttgggtc gtttgttcat
      721 aaacgcgggg ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg
      781 ggccaatacg cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc
      841 ccagggctcg cagccaacgt cggggcggca gggccctgcc ata
//
