LOCUS       sequence-32475-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 cand181
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    107..131
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(527..551)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             577..618
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(658..677)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    702..750
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 ctttagcaaa ctctctggct actagaagaa cccactgcct tactgggctt atcgaaatta
       61 atacgactca ctatagggag acccaagctg gctagttaag ctatcaacaa gtttgtacaa
      121 aaaagcaggc tcatatgtct ccaccaggca tttcttcctc tggggcccca tacttgctcc
      181 cctgagccat gtctccacct tccttctgcc atccctgctt ggcgtccttg tctctctctc
      241 ccctgcccag tggcctcctt gtctggctca ctgggcagga gccctaatcg gattcgacag
      301 ctgagatatg tgtggcggct aagagcaggc aggagggaag ccagtgggag gatggtatga
      361 ggactgcccc tggcactgcc ccggcctccc aagcaccacc ttgggcctgc tgatttctta
      421 cttggcttgg ccccaaggaa ggggtgccag aggaagctca ggaatgaaca ggctggggtc
      481 tggagactag gagaggagga aacacaccaa ggtcctcagg aagcttaccc agctttcttg
      541 tacaaagtgg ttgatctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
      601 ctcggtctcg attctacgcg taccggttag taatgagttt aaacggggga ggctaactga
      661 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
      721 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
      781 actctgtcga taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt
      841 ccccacccca ccccccaagt tcgggtgaaa gccca
//
