LOCUS       sequence-324757-       10325 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10325)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10325)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtTAX3:T35s
FEATURES             Location/Qualifiers
     source          1..10325
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     321..340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    543..567
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      2820..3072
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    4496..4670
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        4770..4949
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             4971..5765
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5025..5044)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5635..5654
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      5778..6030
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6125..6149
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6413..7042
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7471..8544
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8610..8804
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        9087..9191
                     /gene="bla"
                     /label=AmpR promoter
     CDS             9192..9986
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      join(10157..10325,1..420)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
       61 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
      121 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
      181 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
      241 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
      301 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
      361 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
      421 aacgccagca acgcggcctt tttacggttc ctgtggttgg cacatacaaa tggacgaacg
      481 gataaacctt ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt
      541 aggtttaccc gccaatatat cctgtcaaac actgatagtt taaactccct cggtcacatg
      601 tgcatcctcg taaggaggac gtcgttgtgg ttggtgcttt ccttacattc tgagcctctt
      661 tccttctaat ccactcatct gcatcttctt gtgtccttac taatacctca ttggttccaa
      721 attccctccc tttaagcacc agctcgtttc tgttcttcca cagcctccca agtatccaag
      781 ggactaaagc ctccacattc ttcagatcag gatattcttg tttaagatgt tgaactctat
      841 ggaggtttgt atgaactgat gatctaggac cggataagtt cccttcttca tagcgaactt
      901 attcaaagaa tgttttgtgt atcattcttg ttacattgtt attaatgaaa aaatattatt
      961 ggtcattgga ctgaacacga gtgttaaata tggaccaggc cccaaataag atccattgat
     1021 atatgaatta aataacaaga ataaatcgag tcaccaaacc acttgccttt tttaacgaga
     1081 cttgttcacc aacttgatac aaaagtcatt atcctatgca aatcaataat catacaaaaa
     1141 tatccaataa cactaaaaaa attaaaagaa atggataatt tcacaatatg ttatacgata
     1201 aagaagttac ttttccaaga aattcactga ttttataagc ccacttgcat tagataaatg
     1261 gcaaaaaaaa acaaaaagga aaagaaataa agcacgaaga attctagaaa atacgaaata
     1321 cgcttcaatg cagtgggacc cacggttcaa ttattgccaa ttttcagctc caccgtatat
     1381 ttaaaaaata aaacgataat gctaaaaaaa tataaatcgt aacgatcgtt aaatctcaac
     1441 ggctggatct tatgacgacc gttaaggaaa ttgtggttgt cggacgaagt ccagtaataa
     1501 acggcgtcaa agtggttgca gccggcacac acgagtcgtg tttatcaact caaagcacaa
     1561 atacttttcc tcaacctaaa aataaggcaa ttagccaaaa acaactttgc gtgtaaacaa
     1621 cgctcaatac acgtgtcatt ttattattag ctattgcttc accgccttag ctttctcgtg
     1681 acctagtcgt cctcgtcttt tcttcttctt cttctataaa acaataccca aagagctctt
     1741 cttcttcaca attcagattt caatttctca aaatcttaaa aactttctct caattctctc
     1801 taccgtgatc aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg attttgtttt
     1861 cgttcgatcc caatttcgta tatgttcttt ggtttagatt ctgttaatct tagatcgtag
     1921 acgattttct gggtttgatc gttagatatc atcttaattc tcgattaggg tttcatagat
     1981 atcatccgat ttgttcaaat aatttgagtt ttgtcgaata attactcttc gatttgtgat
     2041 ttctatctag atctggtgtt agtttctagt ttgtgcgatc gaatttgtcg attaatctga
     2101 gtttttctga ttaacagatg cagatcttaa tggggtcatc caagaatgtt atcaaggagt
     2161 tcatgaggtt taaggttcgc atggaaggaa cggtcaatgg gcacgagttt gaaatagaag
     2221 gcgaaggaga ggggaggcca tacgaaggcc acaataccgt aaagcttaag gtaaccaagg
     2281 ggggaccttt gccatttgct tgggatattt tgtcaccaca atttcagtat ggaagcaagg
     2341 tatatgtcaa gcaccctgcc gacataccag actataaaaa gctgtcattt cctgaaggat
     2401 ttaaatggga aagggtcatg aactttgaag atggtggcgt cgttactgta acccaggatt
     2461 ccagtttgca ggatggctgt ttcatctaca aggtcaagtt cattggcgtg aactttcctt
     2521 ccgatggacc tgttatgcaa aagaaaacaa tgggctggga agccagcact gagcgtttgt
     2581 atcctcgtga tggcgtgttg aaaggagaga ttcataaggc tctgaagctg aaagacggtg
     2641 gtcattacct agttgaattc aaaagtattt acatggcaaa gaagcctgtg cagctaccag
     2701 ggtactacta tgttgactcc aaactggata taacaagcca caacgaagat tatacaatcg
     2761 ttgagcagta tgaaagaacc gagggacgcc accatctgtt cctttaagct tgtcaagcag
     2821 atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga
     2881 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca
     2941 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg
     3001 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta
     3061 tgttactaga tcgacgctga gctcgaattc tagtggaggg agagaggatt ttgaggaaat
     3121 aattaattga attacttgta ttattgataa agtaatttag ataagttgtt agtacaactt
     3181 attgcaacta tggatagaca aaaatcactt attttaaggg gagaagtata taacaactta
     3241 ttataaaatt tctgagcagc cggcctcctc caatcaatca tagagagtgg agcccattct
     3301 ggaaaaccaa ggaaccccca acttgcactg gtgcggtggc ccaattcaaa aaaaacgggg
     3361 cccagcaaag taacgccgtt gtaacgattt atcaatccaa actcaaaagg cggcgagatg
     3421 cgtttaactc cgtgaaatta acaaaccgcc aacaacttgc aatttgcaac taccgtttcc
     3481 aagaagaact caaccacaca acgtatccta tcccaaacca cacgcaacta gtgacgcgtc
     3541 ataaggacac gtgtcacaat ttgactggtt aataatttca ccgcttttgc tataaattac
     3601 ctccaatccc cttagcttct tcacaattca gttcccaacc ctaacaattc ttgttcatat
     3661 cgcttctctc tactttcaag gtatgatcca atttctctct tctttctctg taatcctttc
     3721 gttgagtttt gtttccgatc aatcataggt agttttcttg tttcgaagca tgagatctag
     3781 gaattttttg tgattttcca aaattgagat cggtttgaaa ttgaatttta cagcttgaat
     3841 ctcagatctt gttttatcaa tgttttcgat ggcttgcgat gtagatctat gataattgtg
     3901 gttcagtttt gttaggaatc gatttcggtt tagcaattgc agattaatta gggtttccaa
     3961 ttgaattctt cagatccgtt atggaattat gtcaaataat ttattcaaat tggaaattat
     4021 tgttagatcc actcttaatc tgtttgatcc aagcttcaat tagggttttc acttgtttca
     4081 atttcttgtt atggattctg atttatctgt tgatgttaga tccactctta atctgtttga
     4141 tccaagcgtt aattagggtt ttcacttgtt tcaatttcgt gtgttggatt ctgatttatc
     4201 tgttgttgat gtgattacag aatggcaagt gaccaagact tttgtgaatg tagacctttg
     4261 ggtttcttca ttggattacc ctttgctcta ttatcattaa ttttatctct tataggagca
     4321 attatttgga ttatcgggtc aatattgagt tgctgctgtc cttgttgcat atgctgcaca
     4381 ggattgctca atgttgctgt atgtttgata aagcttccag ttcgaattct tagatggttt
     4441 gttaacaaaa ttccttgtta ggcttctcta gctagagtcg atcgacaagc tcgagtttct
     4501 ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata gggtttcgct
     4561 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     4621 aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc gctaagcttg
     4681 aattcttgcg gagagcggag aattaaggga gtcacgttat gacccccgcc gatgacgcgg
     4741 gacaagccgt tttacgtttg gaactgacag aaccgcaacg ttgaaggagc cactcagccg
     4801 cgggtttctg gagtttaatg agctaagcac atacgtcaga aaccattatt gcgcgttcaa
     4861 aagtcgccta aggtcactat cagctagcaa atatttcttg tcaaaaatgc tccactgacg
     4921 ttccataaat tcccctcggt atccaattag agtctcatat tcactctcaa atgattgaac
     4981 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
     5041 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
     5101 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactc caagacgagg
     5161 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
     5221 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
     5281 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
     5341 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
     5401 gacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
     5461 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg gatgcccgac ggcgaggatc
     5521 tcgtcgtgac ccagggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     5581 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     5641 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     5701 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     5761 tctgagcttg tcaagcagat cgttcaaaca tttggcaata aagtttctta agattgaatc
     5821 ctgttgccgg tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa
     5881 taattaacat gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc
     5941 aattatacat ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat
     6001 cgcgcgcggt gtcatctatg ttactagatc gacgctggat ccgaattcgg cagggcccac
     6061 tgcatccacc ccagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca
     6121 atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc
     6181 tcggcacaaa atcaccactc gatacaggca gcccatcagc cgtgcggctg catgaaatcc
     6241 tggccggttt gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa
     6301 ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg
     6361 tcgctgcgta tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt
     6421 tatcgctgta cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc
     6481 ccgcgccctg caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag
     6541 tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg
     6601 cccgacgatt gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg
     6661 agcgccccag gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat
     6721 tccggtgcag ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa
     6781 gcagcgcatt gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat
     6841 caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat
     6901 tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac
     6961 cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga
     7021 aattaaatca aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac
     7081 acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg
     7141 gcagacacgc cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag
     7201 ctgaagatgt acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc
     7261 gcgcagctac cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa
     7321 aggaggcggc atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg
     7381 tggaggaacg ggcggttggc caggcgtaag cggctgggtt gtctgccggc cctgcaatgg
     7441 cactggaacc cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta
     7501 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
     7561 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
     7621 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgtcgtcgat taggaagccg
     7681 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
     7741 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
     7801 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
     7861 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
     7921 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
     7981 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
     8041 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
     8101 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
     8161 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
     8221 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
     8281 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
     8341 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
     8401 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
     8461 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
     8521 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
     8581 ggggaaaaag gtcgaaaaag cttctttcct gtggatagca cgtacattgg gaacccaaag
     8641 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
     8701 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
     8761 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
     8821 acagccgaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
     8881 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
     8941 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
     9001 ggcaccgagc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca
     9061 aaaatggcag cgctggccaa ttcgtgcgcg gaacccctat ttgtttattt ttctaaatac
     9121 attcaaatat gtatccgctc atgagacaat aaccttgata aatgcttcaa taatattgaa
     9181 aaaggaagag tatggctaaa atgagaatat caccggaatt gaaaaaactg atcgaaaaat
     9241 accgctgcgc aaaagatacg gaaggaatgt ctcctgctaa ggtatataag ctggtgggag
     9301 aaaatgaaaa cctatattta aaaatgacgg acagccggta taaagggacc acctatgatg
     9361 tggaacggga aaaggacatg atgctatggc tggaaggaaa gctgcctgtt ccaaaggtcc
     9421 tgcactttga acggcatgat ggctggagca atctgctcat gagtgaggcc gatggcgtcc
     9481 tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa gattatcgag ctgtatgcgg
     9541 agtgcatcag gctctttcac tccatcgaca tatcggattg tccctatacg aatagcttag
     9601 acagccgctt agccgaattg gattacttac tgaataacga tctggccgat gtggattgcg
     9661 aaaactggga agaggacact ccatttaaag atccgcgcga gctgtatgat tttttaaaga
     9721 cggaaaagcc cgaagaggaa cttgtctttt cccacggcga cctgggagac agcaacatct
     9781 ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg gagaagcggc agggcggaca
     9841 agtggtatga cattgccttc tgcgtccggt cgatcaggga ggatatcggg gaagaacagt
     9901 atgtcgagct attttttgac ttactgggga tcaagcctga ttgggagaaa ataaaatatt
     9961 atattttact ggatgaattg ttttagctgt cagaccaagt ttactcatat atactttaga
    10021 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    10081 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    10141 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    10201 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    10261 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
    10321 agtta
//
