LOCUS       sequence-324758-       10346 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10346)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtPIP4:T35s
FEATURES             Location/Qualifiers
     source          1..10346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        157..336
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             358..1152
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(412..431)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1022..1041
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1165..1417
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    1512..1536
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1800..2429
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2858..3931
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3997..4191
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        4474..4578
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4579..5373
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5544..6132
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6033..6052
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    6255..6279
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      8532..8784
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    join(10229..10346,1..57)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcgct
       61 aagcttgaat tcttgcggag agcggagaat taagggagtc acgttatgac ccccgccgat
      121 gacgcgggac aagccgtttt acgtttggaa ctgacagaac cgcaacgttg aaggagccac
      181 tcagccgcgg gtttctggag tttaatgagc taagcacata cgtcagaaac cattattgcg
      241 cgttcaaaag tcgcctaagg tcactatcag ctagcaaata tttcttgtca aaaatgctcc
      301 actgacgttc cataaattcc cctcggtatc caattagagt ctcatattca ctctcaaatg
      361 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
      421 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
      481 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactccaa
      541 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
      601 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
      661 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
      721 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
      781 gagcgaggac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
      841 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcggat gcccgacggc
      901 gaggatctcg tcgtgaccca gggcgatgcc tgcttgccga atatcatggt ggaaaatggc
      961 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     1021 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     1081 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     1141 gagttcttct gagcttgtca agcagatcgt tcaaacattt ggcaataaag tttcttaaga
     1201 ttgaatcctg ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag
     1261 catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga
     1321 gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat
     1381 aaattatcgc gcgcggtgtc atctatgtta ctagatcgac gctggatccg aattcggcag
     1441 ggcccactgc atccacccca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     1501 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     1561 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagccgt gcggctgcat
     1621 gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct
     1681 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     1741 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     1801 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     1861 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     1921 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     1981 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     2041 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     2101 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     2161 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     2221 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     2281 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     2341 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     2401 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     2461 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     2521 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     2581 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     2641 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     2701 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     2761 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
     2821 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     2881 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     2941 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     3001 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgt cgtcgattag
     3061 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     3121 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     3181 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     3241 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     3301 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     3361 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     3421 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     3481 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     3541 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg
     3601 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     3661 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     3721 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     3781 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     3841 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     3901 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     3961 cctagcaggg gaaaaaggtc gaaaaagctt ctttcctgtg gatagcacgt acattgggaa
     4021 cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg
     4081 gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta
     4141 aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg
     4201 ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg
     4261 ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg
     4321 gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc
     4381 acatcaaggc accgagctcc catcccccca ggggctgcgc ccctcggccg cgaacggcct
     4441 caccccaaaa atggcagcgc tggccaattc gtgcgcggaa cccctatttg tttatttttc
     4501 taaatacatt caaatatgta tccgctcatg agacaataac cttgataaat gcttcaataa
     4561 tattgaaaaa ggaagagtat ggctaaaatg agaatatcac cggaattgaa aaaactgatc
     4621 gaaaaatacc gctgcgcaaa agatacggaa ggaatgtctc ctgctaaggt atataagctg
     4681 gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa agggaccacc
     4741 tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct gcctgttcca
     4801 aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag tgaggccgat
     4861 ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat tatcgagctg
     4921 tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc ctatacgaat
     4981 agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct ggccgatgtg
     5041 gattgcgaaa actgggaaga ggacactcca tttaaagatc cgcgcgagct gtatgatttt
     5101 ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct gggagacagc
     5161 aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag aagcggcagg
     5221 gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga tatcggggaa
     5281 gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg ggagaaaata
     5341 aaatattata ttttactgga tgaattgttt tagctgtcag accaagttta ctcatatata
     5401 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5461 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5521 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     5581 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     5641 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     5701 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     5761 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     5821 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     5881 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     5941 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6001 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6061 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6121 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctgtggtt ggcacataca
     6181 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg
     6241 ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactcc
     6301 ctcggtcaca tgtgcatcct cgtaaggagg acgtcgttgt ggttggtgct ttccttacat
     6361 tctgagcctc tttccttcta atccactcat ctgcatcttc ttgtgtcctt actaatacct
     6421 cattggttcc aaattccctc cctttaagca ccagctcgtt tctgttcttc cacagcctcc
     6481 caagtatcca agggactaaa gcctccacat tcttcagatc aggatattct tgtttaagat
     6541 gttgaactct atggaggttt gtatgaactg atgatctagg accggataag ttcccttctt
     6601 catagcgaac ttattcaaag aatgttttgt gtatcattct tgttacattg ttattaatga
     6661 aaaaatatta ttggtcattg gactgaacac gagtgttaaa tatggaccag gccccaaata
     6721 agatccattg atatatgaat taaataacaa gaataaatcg agtcaccaaa ccacttgcct
     6781 tttttaacga gacttgttca ccaacttgat acaaaagtca ttatcctatg caaatcaata
     6841 atcatacaaa aatatccaat aacactaaaa aaattaaaag aaatggataa tttcacaata
     6901 tgttatacga taaagaagtt acttttccaa gaaattcact gattttataa gcccacttgc
     6961 attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat aaagcacgaa gaattctaga
     7021 aaatacgaaa tacgcttcaa tgcagtggga cccacggttc aattattgcc aattttcagc
     7081 tccaccgtat atttaaaaaa taaaacgata atgctaaaaa aatataaatc gtaacgatcg
     7141 ttaaatctca acggctggat cttatgacga ccgttaagga aattgtggtt gtcggacgaa
     7201 gtccagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg tgtttatcaa
     7261 ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa aaacaacttt
     7321 gcgtgtaaac aacgctcaat acacgtgtca ttttattatt agctattgct tcaccgcctt
     7381 agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata aaacaatacc
     7441 caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta aaaactttct
     7501 ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc tcaaaatctt
     7561 cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga ttctgttaat
     7621 cttagatcgt agacgatttt ctgggtttga tcgttagata tcatcttaat tctcgattag
     7681 ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa taattactct
     7741 tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga tcgaatttgt
     7801 cgattaatct gagtttttct gattaacaga tgcagatctt aatggggtca tccaagaatg
     7861 ttatcaagga gttcatgagg tttaaggttc gcatggaagg aacggtcaat gggcacgagt
     7921 ttgaaataga aggcgaagga gaggggaggc catacgaagg ccacaatacc gtaaagctta
     7981 aggtaaccaa ggggggacct ttgccatttg cttgggatat tttgtcacca caatttcagt
     8041 atggaagcaa ggtatatgtc aagcaccctg ccgacatacc agactataaa aagctgtcat
     8101 ttcctgaagg atttaaatgg gaaagggtca tgaactttga agatggtggc gtcgttactg
     8161 taacccagga ttccagtttg caggatggct gtttcatcta caaggtcaag ttcattggcg
     8221 tgaactttcc ttccgatgga cctgttatgc aaaagaaaac aatgggctgg gaagccagca
     8281 ctgagcgttt gtatcctcgt gatggcgtgt tgaaaggaga gattcataag gctctgaagc
     8341 tgaaagacgg tggtcattac ctagttgaat tcaaaagtat ttacatggca aagaagcctg
     8401 tgcagctacc agggtactac tatgttgact ccaaactgga tataacaagc cacaacgaag
     8461 attatacaat cgttgagcag tatgaaagaa ccgagggacg ccaccatctg ttcctttaag
     8521 cttgtcaagc agatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     8581 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     8641 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     8701 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     8761 cggtgtcatc tatgttacta gatcgacgct gagctcgaat tctagtggag ggagagagga
     8821 ttttgaggaa ataattaatt gaattacttg tattattgat aaagtaattt agataagttg
     8881 ttagtacaac ttattgcaac tatggataga caaaaatcac ttattttaag gggagaagta
     8941 tataacaact tattataaaa tttctgagca gccggcctcc tccaatcaat catagagagt
     9001 ggagcccatt ctggaaaacc aaggaacccc caacttgcac tggtgcggtg gcccaattca
     9061 aaaaaaacgg ggcccagcaa agtaacgccg ttgtaacgat ttatcaatcc aaactcaaaa
     9121 ggcggcgaga tgcgtttaac tccgtgaaat taacaaaccg ccaacaactt gcaatttgca
     9181 actaccgttt ccaagaagaa ctcaaccaca caacgtatcc tatcccaaac cacacgcaac
     9241 tagtgacgcg tcataaggac acgtgtcaca atttgactgg ttaataattt caccgctttt
     9301 gctataaatt acctccaatc cccttagctt cttcacaatt cagttcccaa ccctaacaat
     9361 tcttgttcat atcgcttctc tctactttca aggtatgatc caatttctct cttctttctc
     9421 tgtaatcctt tcgttgagtt ttgtttccga tcaatcatag gtagttttct tgtttcgaag
     9481 catgagatct aggaattttt tgtgattttc caaaattgag atcggtttga aattgaattt
     9541 tacagcttga atctcagatc ttgttttatc aatgttttcg atggcttgcg atgtagatct
     9601 atgataattg tggttcagtt ttgttaggaa tcgatttcgg tttagcaatt gcagattaat
     9661 tagggtttcc aattgaattc ttcagatccg ttatggaatt atgtcaaata atttattcaa
     9721 attggaaatt attgttagat ccactcttaa tctgtttgat ccaagcttca attagggttt
     9781 tcacttgttt caatttcttg ttatggattc tgatttatct gttgatgtta gatccactct
     9841 taatctgttt gatccaagcg ttaattaggg ttttcacttg tttcaatttc gtgtgttgga
     9901 ttctgattta tctgttgttg atgtgattac agaatgaagc tttctatttt acttccaata
     9961 gttatcatcg tgttggctgt aacaaggcca aacacagtaa attgtagagg ctatccctcc
    10021 caagtaaagg ccattgatga acctatcaat gaactaaagt taaaggttat tgatgaacct
    10081 atcaaagagc taaagatcat gaaagagaag gccaacaatg ttttcagggt tttgattggt
    10141 ggccaagtcc atactatgac ttcagggccc agtgaaaaag gcccaggtca ttaagcttct
    10201 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
    10261 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
    10321 agtatgtatt tgtatttgta aaatac
//
