LOCUS       sequence-324759-       10550 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtLEGIN13:T35s
FEATURES             Location/Qualifiers
     source          1..10550
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      986..1180
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        1463..1567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1568..2362
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      2533..3121
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3022..3041
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    3244..3268
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      5521..5773
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     polyA_signal    7422..7596
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        7696..7875
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             7897..8691
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7951..7970)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8561..8580
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      8704..8956
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    9051..9075
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9339..9968
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             join(10397..10550,1..920)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgtc gtcgattagg aagccgccca
       61 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
      121 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
      181 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
      241 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
      301 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
      361 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
      421 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
      481 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
      541 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
      601 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
      661 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
      721 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
      781 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
      841 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
      901 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
      961 aaaaaggtcg aaaaagcttc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
     1021 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
     1081 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
     1141 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
     1201 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
     1261 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
     1321 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
     1381 ccgagctccc atccccccag gggctgcgcc cctcggccgc gaacggcctc accccaaaaa
     1441 tggcagcgct ggccaattcg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1501 aaatatgtat ccgctcatga gacaataacc ttgataaatg cttcaataat attgaaaaag
     1561 gaagagtatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
     1621 ctgcgcaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
     1681 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
     1741 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
     1801 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
     1861 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
     1921 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
     1981 ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
     2041 ctgggaagag gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
     2101 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
     2161 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
     2221 gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt
     2281 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
     2341 tttactggat gaattgtttt agctgtcaga ccaagtttac tcatatatac tttagattga
     2401 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     2461 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     2521 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     2581 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     2641 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     2701 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     2761 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     2821 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     2881 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     2941 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3001 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3061 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3121 aaacgccagc aacgcggcct ttttacggtt cctgtggttg gcacatacaa atggacgaac
     3181 ggataaacct tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct
     3241 taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactccc tcggtcacat
     3301 gtgcatcctc gtaaggagga cgtcgttgtg gttggtgctt tccttacatt ctgagcctct
     3361 ttccttctaa tccactcatc tgcatcttct tgtgtcctta ctaatacctc attggttcca
     3421 aattccctcc ctttaagcac cagctcgttt ctgttcttcc acagcctccc aagtatccaa
     3481 gggactaaag cctccacatt cttcagatca ggatattctt gtttaagatg ttgaactcta
     3541 tggaggtttg tatgaactga tgatctagga ccggataagt tcccttcttc atagcgaact
     3601 tattcaaaga atgttttgtg tatcattctt gttacattgt tattaatgaa aaaatattat
     3661 tggtcattgg actgaacacg agtgttaaat atggaccagg ccccaaataa gatccattga
     3721 tatatgaatt aaataacaag aataaatcga gtcaccaaac cacttgcctt ttttaacgag
     3781 acttgttcac caacttgata caaaagtcat tatcctatgc aaatcaataa tcatacaaaa
     3841 atatccaata acactaaaaa aattaaaaga aatggataat ttcacaatat gttatacgat
     3901 aaagaagtta cttttccaag aaattcactg attttataag cccacttgca ttagataaat
     3961 ggcaaaaaaa aacaaaaagg aaaagaaata aagcacgaag aattctagaa aatacgaaat
     4021 acgcttcaat gcagtgggac ccacggttca attattgcca attttcagct ccaccgtata
     4081 tttaaaaaat aaaacgataa tgctaaaaaa atataaatcg taacgatcgt taaatctcaa
     4141 cggctggatc ttatgacgac cgttaaggaa attgtggttg tcggacgaag tccagtaata
     4201 aacggcgtca aagtggttgc agccggcaca cacgagtcgt gtttatcaac tcaaagcaca
     4261 aatacttttc ctcaacctaa aaataaggca attagccaaa aacaactttg cgtgtaaaca
     4321 acgctcaata cacgtgtcat tttattatta gctattgctt caccgcctta gctttctcgt
     4381 gacctagtcg tcctcgtctt ttcttcttct tcttctataa aacaataccc aaagagctct
     4441 tcttcttcac aattcagatt tcaatttctc aaaatcttaa aaactttctc tcaattctct
     4501 ctaccgtgat caaggtaaat ttctgtgttc cttattctct caaaatcttc gattttgttt
     4561 tcgttcgatc ccaatttcgt atatgttctt tggtttagat tctgttaatc ttagatcgta
     4621 gacgattttc tgggtttgat cgttagatat catcttaatt ctcgattagg gtttcataga
     4681 tatcatccga tttgttcaaa taatttgagt tttgtcgaat aattactctt cgatttgtga
     4741 tttctatcta gatctggtgt tagtttctag tttgtgcgat cgaatttgtc gattaatctg
     4801 agtttttctg attaacagat gcagatctta atggggtcat ccaagaatgt tatcaaggag
     4861 ttcatgaggt ttaaggttcg catggaagga acggtcaatg ggcacgagtt tgaaatagaa
     4921 ggcgaaggag aggggaggcc atacgaaggc cacaataccg taaagcttaa ggtaaccaag
     4981 gggggacctt tgccatttgc ttgggatatt ttgtcaccac aatttcagta tggaagcaag
     5041 gtatatgtca agcaccctgc cgacatacca gactataaaa agctgtcatt tcctgaagga
     5101 tttaaatggg aaagggtcat gaactttgaa gatggtggcg tcgttactgt aacccaggat
     5161 tccagtttgc aggatggctg tttcatctac aaggtcaagt tcattggcgt gaactttcct
     5221 tccgatggac ctgttatgca aaagaaaaca atgggctggg aagccagcac tgagcgtttg
     5281 tatcctcgtg atggcgtgtt gaaaggagag attcataagg ctctgaagct gaaagacggt
     5341 ggtcattacc tagttgaatt caaaagtatt tacatggcaa agaagcctgt gcagctacca
     5401 gggtactact atgttgactc caaactggat ataacaagcc acaacgaaga ttatacaatc
     5461 gttgagcagt atgaaagaac cgagggacgc caccatctgt tcctttaagc ttgtcaagca
     5521 gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg
     5581 atgattatca tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc
     5641 atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac
     5701 gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct
     5761 atgttactag atcgacgctg agctcgaatt ctagtggagg gagagaggat tttgaggaaa
     5821 taattaattg aattacttgt attattgata aagtaattta gataagttgt tagtacaact
     5881 tattgcaact atggatagac aaaaatcact tattttaagg ggagaagtat ataacaactt
     5941 attataaaat ttctgagcag ccggcctcct ccaatcaatc atagagagtg gagcccattc
     6001 tggaaaacca aggaaccccc aacttgcact ggtgcggtgg cccaattcaa aaaaaacggg
     6061 gcccagcaaa gtaacgccgt tgtaacgatt tatcaatcca aactcaaaag gcggcgagat
     6121 gcgtttaact ccgtgaaatt aacaaaccgc caacaacttg caatttgcaa ctaccgtttc
     6181 caagaagaac tcaaccacac aacgtatcct atcccaaacc acacgcaact agtgacgcgt
     6241 cataaggaca cgtgtcacaa tttgactggt taataatttc accgcttttg ctataaatta
     6301 cctccaatcc ccttagcttc ttcacaattc agttcccaac cctaacaatt cttgttcata
     6361 tcgcttctct ctactttcaa ggtatgatcc aatttctctc ttctttctct gtaatccttt
     6421 cgttgagttt tgtttccgat caatcatagg tagttttctt gtttcgaagc atgagatcta
     6481 ggaatttttt gtgattttcc aaaattgaga tcggtttgaa attgaatttt acagcttgaa
     6541 tctcagatct tgttttatca atgttttcga tggcttgcga tgtagatcta tgataattgt
     6601 ggttcagttt tgttaggaat cgatttcggt ttagcaattg cagattaatt agggtttcca
     6661 attgaattct tcagatccgt tatggaatta tgtcaaataa tttattcaaa ttggaaatta
     6721 ttgttagatc cactcttaat ctgtttgatc caagcttcaa ttagggtttt cacttgtttc
     6781 aatttcttgt tatggattct gatttatctg ttgatgttag atccactctt aatctgtttg
     6841 atccaagcgt taattagggt tttcacttgt ttcaatttcg tgtgttggat tctgatttat
     6901 ctgttgttga tgtgattaca gaatgactta tgttaagctc attactttgg ctctattcct
     6961 ggttaccaca ctcttaatgt ttcagacaaa gaatgttgaa gcagagttct gttcgtccgt
     7021 gggatccttt tgttcaccat ttaacacgaa tccatgtggc tacctcggaa attgtcgctg
     7081 tgtcccttat tatctatatg gaggtacttg cgagaatcca tttggttttg aacataatat
     7141 gaagatgatc gaggaacatc ctaacttatg tcaaactcat gccgagtgca tcaagaaggg
     7201 aagtgggaac ttttgtgctc gttatgctaa tgctgatgtc gaatatgggt ggtgttttgc
     7261 ctctgtagcc gaagcagaac ggtacttcaa aattggatca aacactgcag taaaaagttt
     7321 gttcaagatt gcctctaaat ctaaagaaca agactacctt aagatggctt tggaaattgc
     7381 cacttaagct tctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt
     7441 gagtagttcc cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat
     7501 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa
     7561 ttcctaaaac caaaatccag tactaaaatc cagatcgcta agcttgaatt cttgcggaga
     7621 gcggagaatt aagggagtca cgttatgacc cccgccgatg acgcgggaca agccgtttta
     7681 cgtttggaac tgacagaacc gcaacgttga aggagccact cagccgcggg tttctggagt
     7741 ttaatgagct aagcacatac gtcagaaacc attattgcgc gttcaaaagt cgcctaaggt
     7801 cactatcagc tagcaaatat ttcttgtcaa aaatgctcca ctgacgttcc ataaattccc
     7861 ctcggtatcc aattagagtc tcatattcac tctcaaatga ttgaacaaga tggattgcac
     7921 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     7981 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     8041 gtcaagaccg acctgtccgg tgccctgaat gaactccaag acgaggcagc gcggctatcg
     8101 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     8161 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     8221 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     8281 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgaggacg tactcggatg
     8341 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     8401 gaactgttcg ccaggctcaa ggcgcggatg cccgacggcg aggatctcgt cgtgacccag
     8461 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     8521 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
     8581 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
     8641 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg agcttgtcaa
     8701 gcagatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
     8761 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
     8821 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
     8881 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
     8941 tctatgttac tagatcgacg ctggatccga attcggcagg gcccactgca tccaccccag
     9001 tacattaaaa acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca
     9061 caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca
     9121 ccactcgata caggcagccc atcagccgtg cggctgcatg aaatcctggc cggtttgtct
     9181 gatgccaagc tggcggcctg gccggccagc ttggccgctg aagaaaccga gcgccgccgt
     9241 ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg
     9301 atgcgatgag taaataaaca aatacgcaag gggaacgcat gaaggttatc gctgtactta
     9361 accagaaagg cgggtcaggc aagacgacca tcgcaaccca tctagcccgc gccctgcaac
     9421 tcgccggggc cgatgttctg ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg
     9481 cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc
     9541 gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg
     9601 cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt gctgattccg gtgcagccaa
     9661 gcccttacga catatgggcc accgccgacc tggtggagct ggttaagcag cgcattgagg
     9721 tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca
     9781 tcggcggtga ggttgccgag gcgctggccg ggtacgagct gcccattctt gagtcccgta
     9841 tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag
     9901 aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac
     9961 tcatttgagt taatgaggta aagagaaaat gagcaaaagc acaaacacgc taagtgccgg
    10021 ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc agcctggcag acacgccagc
    10081 catgaagcgg gtcaactttc agttgccggc ggaggatcac accaagctga agatgtacgc
    10141 ggtacgccaa ggcaagacca ttaccgagct gctatctgaa tacatcgcgc agctaccaga
    10201 gtaaatgagc aaatgaataa atgagtagat gaattttagc ggctaaagga ggcggcatgg
    10261 aaaatcaaga acaaccaggc accgacgccg tggaatgccc catgtgtgga ggaacgggcg
    10321 gttggccagg cgtaagcggc tgggttgtct gccggccctg caatggcact ggaaccccca
    10381 agcccgagga atcggcgtga gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc
    10441 gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat
    10501 cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
//
