LOCUS       sequence-324765-        2135 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression of ArsR in E. coli for cell-free arsenic sensing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2135)
  TITLE     Sensitizing cell-free molecular detection using cascaded genetic 
            circuitry
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pJBL7043
FEATURES             Location/Qualifiers
     source          1..2135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      17..605
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     506..525
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     633..652
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        633..651
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             683..705
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             713..724
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(1136..1154)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1150..1197
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1215..2030)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1938..1957
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2022..2041
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
       61 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      121 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      181 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      241 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      301 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      361 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      421 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      481 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      541 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      601 ggaaaaacgc cagcaacgcg atcccgcgaa attaatacga ctcactatag ggagaccaca
      661 acggtttccc tctagaaata attttgttta actttaagaa ggagatatac ataaagagga
      721 gaaatactag atgtggcaac taacaccact tcagttattt aaaaacctgt ccgatgaaac
      781 ccgtttgggt atcgtgttgt tgctcaggga gatgggagaa ttgtgcgtgt gtgatctttg
      841 catggcactg gatcaatcac agcccaaaat atcccgtcat ctggcgatgc tacgggtaag
      901 tggaatcctt ctggatcgta aacagggaaa atgggttcac taccgcttat caccccatat
      961 tccttcatgg gccgcccaga ttatagagca ggcctggtta agccaacagg acgacgttca
     1021 ggtcatcgca cgcaagctgg cttcagttaa ctgctccggt agcagtaagg ctgtctgcat
     1081 ctaagtcgac cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     1141 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     1201 aaagccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     1261 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     1321 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     1381 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     1441 ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact
     1501 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     1561 ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta
     1621 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     1681 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
     1741 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
     1801 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
     1861 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
     1921 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
     1981 gaatttaatc gcggcttcga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
     2041 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
     2101 gcaatgtaac atcagagatt ttgagacaca acgtg
//
