LOCUS       sequence-32479-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-137
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     41..60
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        41..59
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    89..113
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(427..451)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             477..518
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(558..577)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    602..650
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tggctactag agaacccact gcttactggc ttatcgaaat taatacgact cactataggg
       61 agacccaagc tggctagtta agctatcaac aagtttgtac aaaaaagcag gctgctccgc
      121 agcaagagtt ctttctggcg gcggcggcgg tggcggcagc ggcagcggca gcagtgacag
      181 cggtagcaga ggcagaggca gcggcagctt ggccctctga ctctcttcgg tgacgggtat
      241 tcttgggtgg ataatacgga ttacgttgtt attgcttaag aatacgcgta gtcgaggaga
      301 gtaccagcgg caggcgggca gcggccgccc ctcccagccc accagctggc cactaaaagc
      361 tcccggttgc caaggtagca ctttttatgt tttcttttcc tcgggtggtt tcgttctgga
      421 tcttccaccc agctttcttg tacaaagtgg ttgatctaga gggcccgcgg ttcgaaggta
      481 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggttag taatgagttt
      541 aaacggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
      601 acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg
      661 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
      721 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
      781 cgcagccaac gtcggggcgg caggccctgc catagcagat ctgcgcagct ggggctctag
      841 ggggtatccc ccacgcgccc tgtagcggcg catt
//
