LOCUS       sequence-32481-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Chiang HR, Schoenfeld LW, Ruby JG, Auyeung VC, Spies N, Baek D, 
            Johnston WK, Russ C, Luo S, Babiarz JE, Blelloch R, Schroth GP, 
            Nusbaum C, Bartel DP
  TITLE     Mammalian microRNAs: experimental evaluation of novel and previously
            annotated genes.
  JOURNAL   Genes Dev. 2010 May 15. 24(10):992-1009.
  PUBMED    20413612
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2010 May 15. 24(10):992-1009."
COMMENT     Sequence Label: pcDNA3.2/V5 mmu-mir-139
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    90..114
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(434..458)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             484..525
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(565..584)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    609..657
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 tggctactag agaacccact gcttactggc ttatcgaaat taatacgact cacttatagg
       61 gagacccaag ctggctagtt aagctatcaa caagtttgta caaaaaagca ggctggagga
      121 gtgggaggtc caggggggga ccagctatgg taagggatga gagaagccaa cagttgtccc
      181 aagggtcccc ctcagggagt ggaggttccc ctgggaggcg ccaggacagg cgcaggtgta
      241 ttctacagtg cacgtgtctc cagtgtggct cggaggctgg agacgcggcc ctgttggagt
      301 aacaactgaa gccagagtct tcgaagggtg gtgggctaag ggggaagagg gcatggtggg
      361 caaaagaaag ggggtggggg agagagaaaa ggaggacaag ggggcccctt cctgtagtac
      421 ttggctcttg agcacccagc tttcttgtac aaagtggttg atctagaggg cccgcggttc
      481 gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggttagtaa
      541 tgagtttaaa cgggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg
      601 cgctatgacg gcaataaaaa gacagaataa aacgcacggg tgttgggtcg tttgttcata
      661 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg
      721 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc
      781 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcagatctg cgcagctggg
      841 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcn gggtgtggt
//
