LOCUS       sequence-325-exp        6371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6371)
  TITLE     Fire Lab C. Elegans Vector Kit 2005
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD172.01
FEATURES             Location/Qualifiers
     source          1..6371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1176..1204)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1952..1972
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2074..2093)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             3237..3257
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(3811..3833)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3933..3952
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(4030..4048)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4116..4220
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4221..4289,4290..5081)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4439..4458)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5252..5840
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5741..5760
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5994..6011
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6128..6149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6164..6169,6170..6187,6188..6194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6202..6218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6207..6229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6226..6242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6226..6242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgggc tgcagagtgg agagtgtagt ccctcgtcgg ctctctgtgt atttctttca
       61 atgcctgtgt ctcggtctat ctttgtctct atctgtttta aaagtttttt gcaaagtttc
      121 aatttctttt ttattttctc ttcttttttt gcgcatttat tttttttaaa gagcgctgtt
      181 cttcaattgg aaaacagtat aacgatcgaa ataaaaaaaa aacttgaaat agtatcggaa
      241 ttgagatggg ggcaattgaa attgaagacg caacaaaaac atgtttttat cgtctgaaat
      301 tatctaaact ttgaatctaa cgtgtcaatg gctactttta cagtagactt tttagagtaa
      361 tgttaaaaag gataatatag gtttttcttc aaactatttt gtcacgccgc atatattctt
      421 ttcatattcc attcgcaaag tgcatgatgt aacaaaaatg aaacgtcata attttttaat
      481 ctttcacact ttttttgccg gccgttaaat caaaatcaaa tcaacttaat ccaacacgtg
      541 cgggcttgca attttattac accggtgtcc tacagcgcca tgttatcttc tttgattaga
      601 gatctctcac gcaacatttg acattgaaaa ttttgtctcc gaattgttgt gtacctactt
      661 tgtcccgatg tcccaaattt tcactgataa caagagatat ctcaactgtt cttatcacgt
      721 tgatacgtag aacgttttac actaaaccaa attgcagtcc ccttctacga accgtttgct
      781 aattgttttg catagaacag tgatcaatgt gttgtgctca ctagtcaatg atgttcgtct
      841 ctcatttttc ctacaacttc actgaaaagg ttttgaatat ctggtgattg taggcaatca
      901 caccacagtt aatggcctgg caaataatga taagcgtgtg ataaacctca caaaaatacg
      961 cctagaagtg gtgatatcgc tagttttgtg tcctttcgat ttttttcgtg ttgctttttt
     1021 ctgtggataa acctttgaac tttacatttt gttttcagaa taaccgctct agaggatcct
     1081 atcgattcgc ggccgctgta cacccgggtg ccctatagtg agtcgtattg gtaccggtag
     1141 aaaaaatgag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     1201 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     1261 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccatgggtaa
     1321 gtttaaacat atatatacta actaaccctg attatttaaa ttttcagcca acacttgtca
     1381 ctactttctg ttatggtgtt caatgcttct cgagataccc agatcatatg aaacagcatg
     1441 actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag
     1501 atgacgggaa ctacaagaca cgtaagttta aacagttcgg tactaactaa ccatacatat
     1561 ttaaattttc aggtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     1621 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     1681 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttgtaa
     1741 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagaac ttcaaaatta
     1801 gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa
     1861 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt
     1921 cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg
     1981 ggattacaca tggcatggac gaactataca aaagcccaca agctttggaa ttcctgcagc
     2041 ccgggggatc catggtgaaa ccagtaacgt tatacgatgt cgcagagtat gccggtgtct
     2101 cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg
     2161 aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac
     2221 tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc
     2281 cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg
     2341 tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg
     2401 cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg
     2461 tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca
     2521 tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg
     2581 cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc
     2641 tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac
     2701 gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg
     2761 gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg
     2821 ccattaccga gtccgggctg cgcgttggtg cggatgtaag tttaaacacc ttaatactaa
     2881 ctaactgtta ttatttaaat tttcagatct cggtagtggg atacgacgat accgaagaca
     2941 gctcatgtta tatcccgccg tcaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     3001 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     3061 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     3121 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga agcagcctga
     3181 gggtaagttt aaacgatgaa ttactaacta acttgccaaa tttaaatttt cagcctccta
     3241 agaagaagag gaaggtttga gcgcaacgca attaatgtaa gttagctcac tcattaggca
     3301 ccccaggctt tacactttat gcttccgacc tgcagcccgg atgatcgacg ccaacgtcgt
     3361 tgaattttca aattttaaat actgaatatt tgtttttttt cctattattt atttattctc
     3421 tttgtgtttt ttttcttgct ttctaaaaaa ttaattcaat ccaaatctaa acattttttt
     3481 ttctctttcc gtctcccaat tcgtattccg ctcctctcat ctgaacacaa tgtgcaagtt
     3541 tatttatctt ctcgctttca tttcattagg acgtgggggg aattggtgga agggggaaac
     3601 acacaaaagg atgatggaaa tgaaataagg acacacaata tgcaacaaca ttcaattcag
     3661 aaatatggag gaaggtttaa aagaaaacat aaaaatatat agaggaggaa ggaaaactag
     3721 taaaaaataa gcaaagaaat taggcgaacg atgagaattg tcctcgcttg ggccctttcg
     3781 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3841 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3901 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     3961 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     4021 gccttaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     4081 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     4141 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     4201 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     4261 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4321 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4381 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4441 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4501 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4561 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4621 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4681 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4741 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4801 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4861 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4921 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4981 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     5041 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     5101 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     5161 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     5221 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     5281 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5341 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     5401 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5461 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5521 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5581 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5641 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5701 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5761 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5821 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5881 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5941 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6001 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     6061 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     6121 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     6181 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     6241 accatgatta cgccaagctg taagtttaaa catgatctta ctaactaact attctcattt
     6301 aaattttcag agcttaaaaa tggctgaaat cactcacaac gatggatacg ctaacaactt
     6361 ggaaatgaaa t
//
