LOCUS       sequence-325070-       10967 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of Peptide coding gene (Binary vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Scheible W
  TITLE     Scheible Lab plasmids
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: LjUBQ:MtCAPE12:T35s
FEATURES             Location/Qualifiers
     source          1..10967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(679..1308)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    1572..1596
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(1691..1943)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(1956..2750)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2067..2086)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2677..2696
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2772..2951)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     complement(4951..4967)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4951..4967)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4964..4986)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4975..4991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4999..5005,5006..5023,5024..5029))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5044..5065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5182..5199)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5353..5941)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6112..6903)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(7125..7229)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7297..7315
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7353..7375)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7475..7494
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7688..7710
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7702..7719
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7703..7719
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7796..7820
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(7943..8531)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8702..9496)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCAKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     promoter        complement(9497..9601)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9884..10078
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(10144..10967,1..250))
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGASSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 gcatcggaac gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacgacg
       61 caccggctgc cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg
      121 attcaccggg gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca
      181 acttctccac caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc
      241 gaccgctcac gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcagac
      301 aacccagccg cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc
      361 gtcggtgcct ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct
      421 actcatttat tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct
      481 cggtaatggt cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg
      541 gcaactgaaa gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag
      601 ccttgctgct gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt
      661 tctctttacc tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac
      721 ctcgcgggca gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt
      781 gcctgggtag ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc
      841 cagcgcctcg gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa
      901 ggccgcttgt agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc
      961 ggcggtggcc catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc
     1021 cttgatcgcg gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc
     1081 gcgccggccg atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt
     1141 tagcggttga tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc
     1201 gactaacaga acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt
     1261 cgtcttgcct gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg
     1321 cgtatttgtt tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt
     1381 tactcaaata cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg
     1441 gccggccagg ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg
     1501 gctgatgggc tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt
     1561 tggctggctg gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat
     1621 aacacattgc ggacgttttt aatgtactgg ggtggatgca gtgggccctg ccgaattcgg
     1681 atccagcgtc gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg
     1741 cgctatattt tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa
     1801 acccatctca taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc
     1861 aacagaaatt atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt
     1921 tattgccaaa tgtttgaacg atctgcttga caagctcaga agaactcgtc aagaaggcga
     1981 tagaaggcga tgcgctgcga atcgggagcg gcgataccgt aaagcacgag gaagcggtca
     2041 gcccattcgc cgccaagctc ttcagcaata tcacgggtag ccaacgctat gtcctgatag
     2101 cggtccgcca cacccagccg gccacagtcg atgaatccag aaaagcggcc attttccacc
     2161 atgatattcg gcaagcaggc atcgccctgg gtcacgacga gatcctcgcc gtcgggcatc
     2221 cgcgccttga gcctggcgaa cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga
     2281 tcatcctgat cgacaagacc ggcttccatc cgagtacgtc ctcgctcgat gcgatgtttc
     2341 gcttggtggt cgaatgggca ggtagccgga tcaagcgtat gcagccgccg cattgcatca
     2401 gccatgatgg atactttctc ggcaggagca aggtgagatg acaggagatc ctgccccggc
     2461 acttcgccca atagcagcca gtcccttccc gcttcagtga caacgtcgag cacagctgcg
     2521 caaggaacgc ccgtcgtggc cagccacgat agccgcgctg cctcgtcttg gagttcattc
     2581 agggcaccgg acaggtcggt cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg
     2641 aacacggcgg catcagagca gccgattgtc tgttgtgccc agtcatagcc gaatagcctc
     2701 tccacccaag cggccggaga acctgcgtgc aatccatctt gttcaatcat ttgagagtga
     2761 atatgagact ctaattggat accgagggga atttatggaa cgtcagtgga gcatttttga
     2821 caagaaatat ttgctagctg atagtgacct taggcgactt ttgaacgcgc aataatggtt
     2881 tctgacgtat gtgcttagct cattaaactc cagaaacccg cggctgagtg gctccttcaa
     2941 cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc ccgcgtcatc ggcgggggtc
     3001 ataacgtgac tcccttaatt ctccgctctc cgcaagaatt caagcttagc tcatgtggaa
     3061 gcactactat gatgatgctt agtatggcca gcaacattcc tttcctcaat gggacagtgt
     3121 gtaccaccac ttcctgggat aaatgtacag ccagaaggtc cagatggagc cacaggaccc
     3181 ttatcaagag cttgcaacct aagctctttc atgttcaaaa ccctaacacc aaggtttggt
     3241 cgcacatgaa ttatggtaag aagtagtaag aatactaaaa caatgttatt aagagccctc
     3301 attctgtaat cacatcaaca acagataaat cagaatccaa cacacgaaat tgaaacaagt
     3361 gaaaacccta attaacgctt ggatcaaaca gattaagagt ggatctaaca tcaacagata
     3421 aatcagaatc cataacaaga aattgaaaca agtgaaaacc ctaattgaag cttggatcaa
     3481 acagattaag agtggatcta acaataattt ccaatttgaa taaattattt gacataattc
     3541 cataacggat ctgaagaatt caattggaaa ccctaattaa tctgcaattg ctaaaccgaa
     3601 atcgattcct aacaaaactg aaccacaatt atcatagatc tacatcgcaa gccatcgaaa
     3661 acattgataa aacaagatct gagattcaag ctgtaaaatt caatttcaaa ccgatctcaa
     3721 ttttggaaaa tcacaaaaaa ttcctagatc tcatgcttcg aaacaagaaa actacctatg
     3781 attgatcgga aacaaaactc aacgaaagga ttacagagaa agaagagaga aattggatca
     3841 taccttgaaa gtagagagaa gcgatatgaa caagaattgt tagggttggg aactgaattg
     3901 tgaagaagct aaggggattg gaggtaattt atagcaaaag cggtgaaatt attaaccagt
     3961 caaattgtga cacgtgtcct tatgacgcgt cactagttgc gtgtggtttg ggataggata
     4021 cgttgtgtgg ttgagttctt cttggaaacg gtagttgcaa attgcaagtt gttggcggtt
     4081 tgttaatttc acggagttaa acgcatctcg ccgccttttg agtttggatt gataaatcgt
     4141 tacaacggcg ttactttgct gggccccgtt ttttttgaat tgggccaccg caccagtgca
     4201 agttgggggt tccttggttt tccagaatgg gctccactct ctatgattga ttggaggagg
     4261 ccggctgctc agaaatttta taataagttg ttatatactt ctccccttaa aataagtgat
     4321 ttttgtctat ccatagttgc aataagttgt actaacaact tatctaaatt actttatcaa
     4381 taatacaagt aattcaatta attatttcct caaaatcctc tctccctcca ctagaggatg
     4441 cacatgtgac cgagggattg tcttcgtgga aacttgggac ttcagattcc accccttgca
     4501 agcgtggaaa gccttccgac caagggaagt gtcggatgta gaggaaatgg aaagtttgtt
     4561 ctcagatggg gacttgcttg attgcttcac aagaatgcca gcttatgcgg taaacgcaga
     4621 ggaagattta gctgcaatca ggaaaacgcc cgagatggat gtcggtcaag aagttaaaga
     4681 acctgcagga gacagaaatc aatactcaaa ccctgcagaa actttcctca acaagctcca
     4741 caggaaacac agtagggagg tgaaacacca ggccgcaaag aaagctaaac gcctagctga
     4801 aatccaggag tcaatgagag ctgaaggtga tgccgaacca aatgaaataa gcgggggcat
     4861 gggggcaata cccagcaacg ccgaacttcc tgggcccaaa ggggatcctc tagagtcgac
     4921 ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4981 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5041 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5101 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5161 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5221 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5281 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5341 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5401 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5461 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5521 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5581 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5641 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     5701 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     5761 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     5821 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     5881 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     5941 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6001 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6061 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttatttgcc
     6121 gactaccttg gtgatctcgc ctttcacgta gtggacaaat tcttccaact gatctgcgcg
     6181 cgaggccaag cgatcttctt cttgtccaag ataagcctgt ctagcttcaa gtatgacggg
     6241 ctgatactgg gccggcaggc gctccattgc ccagtcggca gcgacatcct tcggcgcgat
     6301 tttgccggtt actgcgctgt accaaatgcg ggacaacgta agcactacat ttcgctcatc
     6361 gccagcccag tcgggcggcg agttccatag cgttaaggtt tcatttagcg cctcaaatag
     6421 atcctgttca ggaaccggat caaagagttc ctccgccgct ggacctacca aggcaacgct
     6481 atgttctctt gcttttgtca gcaagatagc cagatcaatg tcgatcgtgg ctggctcgaa
     6541 gatacctgca agaatgtcat tgcgctgcca ttctccaaat tgcagttcgc gcttagctgg
     6601 ataacgccac ggaatgatgt cgtcgtgcac aacaatggtg acttctacag cgcggagaat
     6661 ctcgctctct ccaggggaag ccgaagtttc caaaaggtcg ttgatcaaag ctcgccgcgt
     6721 tgtttcatca agccttacgg tcaccgtaac cagcaaatca atatcactgt gtggcttcag
     6781 gccgccatcc actgcggagc cgtacaaatg tacggccagc aacgtcggtt cgagatggcg
     6841 ctcgatgacg ccaactacct ctgatagttg agtcgatact tcggcgatca ccgcttccct
     6901 catgatgttt aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctcca
     6961 taacatcaaa catcgaccca cggcgtaacg cgcttgctgc ttggatgccc gaggcataga
     7021 ctgtacccca aaaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca
     7081 ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatactctt cctttttcaa
     7141 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7201 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     7261 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     7321 cgactcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagatg
     7381 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7441 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     7501 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     7561 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     7621 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     7681 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcgagct cggtaccctt
     7741 tgaagacaaa ctagaggatg cacatgtgac cgagggagtt taaactatca gtgtttgaca
     7801 ggatatattg gcgggtaaac ctaagagaaa agagcgttta ttagaataat cggatattta
     7861 aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgccaaccac aggaaccgta
     7921 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7981 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8041 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8101 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8161 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8221 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8281 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8341 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     8401 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8461 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8521 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8581 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8641 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8701 gctaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
     8761 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
     8821 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
     8881 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
     8941 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
     9001 taaatggagt gtcctcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
     9061 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
     9121 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
     9181 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
     9241 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
     9301 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
     9361 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
     9421 ttccttccgt atcttttgcg cagcggtatt tttcgatcag ttttttcaat tccggtgata
     9481 ttctcatttt agccatactc ttcctttttc aatattattg aagcatttat caaggttatt
     9541 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9601 gcacgaattg gccagcgctg ccatttttgg ggtgaggccg ttcgcggccg aggggcgcag
     9661 cccctggggg gatgggagct cggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     9721 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     9781 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     9841 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     9901 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     9961 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
    10021 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
    10081 aaagaagctt tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
    10141 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
    10201 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
    10261 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
    10321 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
    10381 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
    10441 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
    10501 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
    10561 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
    10621 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
    10681 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
    10741 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
    10801 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
    10861 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
    10921 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcataga
//
