LOCUS       sequence-32524-e         219 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 219)
  AUTHORS   Peti W, Page R
  TITLE     Strategies to maximize heterologous protein expression in 
            Escherichia coli with minimal cost.
  JOURNAL   Protein Expr Purif. 2007 Jan . 51(1):1-10.
  PUBMED    16904906
REFERENCE   2  (bases 1 to 219)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif. 2007 Jan . 51(1):1-10."
COMMENT     Sequence Label: RP1B
FEATURES             Location/Qualifiers
     source          1..219
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             49..71
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    86..110
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attttcgtga tgatgatgat gatgaatttt atcagagccc atggtatatc tccttcttaa
       61 agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc cctaaagtga
      121 gtcatattaa tttcgcggga tcgagatctc gatcctctac gccggacgca tcgtggccgg
      181 catcaccggc gccacaggtg cggttgctgg cgcctatat
//
