LOCUS       sequence-325332-        9865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SmoM2-FLAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Desai PB, Stuck MW, Lv B, Pazour GJ
  TITLE     Ubiquitin links smoothened to intraflagellar transport to regulate 
            Hedgehog signaling.
  JOURNAL   J Cell Biol. 2020 Jul 6;219(7). pii: 151798. doi: 
            10.1083/jcb.201912104.
  PUBMED    32435793
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2020-07-6"; volume: "219(7). pii: 151798. doi"; 
            pages: " 10.1083/jcb.201912104"
COMMENT     Sequence Label: PD23
FEATURES             Location/Qualifiers
     source          1..9865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(394..402,403..405,406..979)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(567..586)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     796..813
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(1005..1070)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      1070..1079
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3530..3554)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(3551..3754)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3580..3600)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3755..4058
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    4110..4227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4523..4567)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4752..4985
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5482..5607
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5654..6287
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6479..6498)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6548..6564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6548..6564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6561..6583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6572..6588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6596..6602,6603..6620,6621..6626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6641..6662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6902..6919)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7073..7661)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(7832..8623,8624..8692))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8455..8474
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8693..8797)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8865..8883
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     LTR             8930..9110
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9258..9846
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             9364..9375
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     9773..9793
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(join(9860..9865,1..393))
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 ctcccacaca taaccagagg gcagcaattc acgaatccca actgccgtcg gctgtccatc
       61 actgtccttc actatggctt tgatcccagg atgcagatcg agaagcacct gtcggcaccg
      121 tccgcagggg ctcaagatgc ccctgttctc atttccgatc gcgacgatac aagtcaggtt
      181 gccagctgcc gcagcagcag cagtgcccag caccacgagt tctgcacaag gtcccccagt
      241 aaaatgatat acattgacac cagtgaagat gcggccgtcg ctagagagag ctgcgctggc
      301 gacgctgtag tcttcagaga tggggatgct gttgattgta gccgttgctc tttcaatgag
      361 ggtggattct tcttgagaca aaggcttggc catggttgtg gccatattat catcgtgttt
      421 ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgttttttt aacctcgact
      481 aaacacatgt aaagcatgtg taccgaggcc ccagatcaga tcccatacaa tggggtacct
      541 tctgggcatc cttcagcccc ttgttgaata cgcttgagga gagccatttg actctttcca
      601 caactatcca actcacaacg tggcactggg gttgtgccgc ctttgcaggt gtatcttata
      661 cacgtggctt ttggccgcag aggcacctgt cgccaggtgg ggggttccgc tgcctgcaaa
      721 gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct tccttcacga
      781 cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga atgctcgtca
      841 agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat atggtggaaa
      901 ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca gtaacgttag
      961 ggggggggag ggagaggggc ggatcttact tactaagcgg ccgccttgtc atcgtcatcc
     1021 ttgtaatcga tgtcatgatc tttataatca ccgtcatggt ctttgtagtc catggtggcg
     1081 aagtccgagt ctgcatccaa gatctcagcc tccattaggt tagtgcggga atgaatggat
     1141 cccagcccct ggaggcggcc ctgagcccag acccgggggg ctgaggcacc agggagaaat
     1201 ggatcttgat ggggactagg ctctgggcag aaggggttgg agactagagt ccaggctccc
     1261 tgtcggcagg actcccctgt tccccaggcg tttgcagcta ccaggcactt ctgccgaggc
     1321 agctgaggca gccgaggaac tgcactggtg gcaggaacag gggcagagtg gtgaagctca
     1381 ggggctggcc ccaaggggca cacctccttc ttcctcttcc tccgcttttt cttccggccc
     1441 aaacgcttct ctaactctgg ggagatctct gcctcaacca gccacatgtt ggcttgttct
     1501 tctggtggca ctggagttgc cacaggggta acggacacat cctggggcaa tatggctcct
     1561 ctccgagcca ccatcttggt gacatgctga gcccaggcag aggagacgtc agctgagggt
     1621 tcattgaggt caaaagccaa acccgcaaca ggtccatcat gggagacagt gtgcatgctg
     1681 aaggagagct cctggcccgg gttctgcagc agctcacgcc gcttagagaa ggccttggcg
     1741 atcatcttgc tcttcttgat tctcttgggc tcatcatcac tgtgcccagt caacctgcac
     1801 caggtgcgcc tccagatgag cagggtggcc ttggtccaga ccaaggtgct catggcaatg
     1861 ccagtgccaa acatggcaaa tagattgatc ttctccacca ggaggctggg ccgattcttg
     1921 atctcacagt caggaatggg cttcttggta ggcagcccga tggtcacgtt ggcttggcat
     1981 agcacatagt cccggaagct acgctcccac tcagcctggt tgaagaagtc atagaagtgg
     2041 cagctgaagg tgatgagcac aaagccaaag gccaggaagc caaaaatgcc caggcgcagc
     2101 atggtctcgt tgatcttgct ggctgccttc tcactcagaa gcccagggtg gttgctcttg
     2161 atggagaaca gagtcatgac ccctctgatg aggaagtagc ctcccacaat aagcaccagg
     2221 ccaattgggg ccaggacaaa gccagcacgg taccgatagt tcttgtagcc tacaaaacag
     2281 atgccactca cggagtctcc atctacctga gccacagcca ggattgccac cgtgaggaca
     2341 aaggggagtg accacgtgag caggtggaaa taggatgtct tgcccgagag aggctggtag
     2401 gtggtgccca gggctttgaa ggaggtgtgc caggcatagg tgaggaccac gaaccagact
     2461 actccagcca tcaaggcata gtacacaatg acaaagatga tgacacagga cagggtctca
     2521 ctggaggtgg gctccccaaa tctcatggtg ccatctgctc ggcaaacaat ctctcggcgg
     2581 gcaccatcca tgaactgggc cagccagcca atgctgccca cgaagaaaca cgcattgaca
     2641 tagaagagaa taaccgcagg gtagcgattg gagttccgcc agtcagccac aaaggtggcc
     2701 agtgtgaaga gcgtgcagag accagtgacc gccccgaagg ctgcgatgta gctgtgcatg
     2761 tcctggtgct cggcctcggt gaacagcggg ttctgacact gaatcccaca gccctccacg
     2821 tcctcatacc agctcttggg gttgtctgtt cgcaccaagg gtgcttcaca ttggcctgag
     2881 ctgttgaact tgatgttttg tacctcgttt gggcagcctt cagggaagtg gtccggtgtg
     2941 caacgcagaa agtcaggcca ccctcgctcc cgctccacaa tggcacaggg gcctcgggtg
     3001 gcctggcaga gggtacggct gggcaactcc actcggtcat tctcacactt gggcatgtag
     3061 acagcacaca gcaggggctg gatcactgcc cagcagcggg gggcattccg gaggccggac
     3121 cagagcacga gcttgccgtg cgcttcctcc tgcgagtccg agtccccagc cagcagcgtg
     3181 gtggtggctc cgtagggcag cgccgagccc aggcacacgt tgtagcgcaa aggctcgcag
     3241 tgggcggccc ggccgcagtg gctcagcagc ggcggcggag ggctggtcac cggcacgtcc
     3301 ctcctcgagc tcccgctggc gctgtgaggc ccaggcccgg tcacgttccc gctcaaggcc
     3361 gccccccggc ccgggccccc cagcagtacc agcagcagca actgcagcag cctccggggc
     3421 gccagctcgg gcccacgcac ggggcggcca gcggccatgg tctgatcatt aattaaggcc
     3481 gccggtgtct tgaatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt
     3541 tcactaaacg agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     3601 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     3661 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     3721 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     3781 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     3841 gccattttgc gtcattgacg tcaatagggg gcgttgttgg caaatgatac acttgatgta
     3901 ctgccaagtg ggcagttttg cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     3961 ttggcgttac tatgggaaca ttggtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     4021 gtcagccagg cgggccattt tgcgtaagtt atgtaacgcg gaactccact agtccaaact
     4081 ggatctctgc tgtccgagta ataaacccga aaattttgaa tttttgtaat ttgtttttgt
     4141 aattctttag tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac
     4201 cccccaatcc ccccttttct tttaaaattg tggatgaata ctgcctattg ttgagaattc
     4261 ggcgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac
     4321 cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa
     4381 acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta
     4441 ttcttaaacc taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat
     4501 ttgttatgtt aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt
     4561 cattcttttc ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat
     4621 tgttaatttc tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt
     4681 tattactcca actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac
     4741 cccaaatccc caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct
     4801 ggagctgctt gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc
     4861 tcagcaaatt gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca
     4921 gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag
     4981 ctcctattcc cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg
     5041 ctactcctaa tggttcaatt tttactactt tatatttata taattcactt ctccaattgt
     5101 ccctcatatc tcctcctcca ggtctgaaga tcagcggccg gccgcttgct gtgcggtggt
     5161 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     5221 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     5281 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     5341 ttctaacagg ccaggattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     5401 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     5461 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     5521 agtcaaaatt tttggcgtac tctgcagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     5581 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     5641 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     5701 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     5761 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     5821 aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg
     5881 ccactcccca gtcccgccca ggccacgcct ccctggaaag tccccagcgg aaagtccctt
     5941 gtagcaagct cgatatcagc agttcttgaa gtactccgga tgcagctctc gggccacgtg
     6001 atgaaatgct aggcggctgt caaacctcca ctctaacact tctctctccg ggtcatccat
     6061 cccatgcagg ctcacagggt gtaacaagct ggtgttctct cctttattgg cctcttctac
     6121 cttatctggc tcaactggta ctagcttgta gcaccatcca aaggtcagtg gatatctgac
     6181 ccctggccct ggtgtgtagt tctgctaatc agggaagtag ccttgtgtgt ggtagatcca
     6241 cagatcaagg atatcttgtc ttctttggga gtgaattagc ccttccaact actaagtttg
     6301 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg
     6361 tttagagtaa atgaaaccac aggtaattgg cagtggtaat agggtatggg gtgggaagtt
     6421 tgggatgatt ttggttagct tgagttatcc agttgatcca gacatgataa gatacattga
     6481 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaattcg
     6541 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6601 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6661 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cacggggagc
     6721 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     6781 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg agcttgctgc
     6841 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6901 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6961 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7021 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7081 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7141 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7201 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7261 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7321 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7381 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7441 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7501 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7561 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7621 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7681 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7741 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7801 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7861 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7921 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7981 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8041 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8101 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8161 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8221 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8281 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8341 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8401 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     8461 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8521 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8581 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8641 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8701 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8761 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     8821 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     8881 ggccctttcg tcttcaagaa tgatctagcc ctttccttaa tcgcccgggt gctagagatt
     8941 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     9001 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     9061 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaagca
     9121 aaaagcaggg cgcgcctgtc ctgcaggtga tcttgtcttc gttgggagtg aattagccct
     9181 tccagtcccc ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt
     9241 ggtcttaaag gtaccaggcg gggaggcggc ccaaagggag atccgactcg tctgagggcg
     9301 aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc
     9361 tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac
     9421 acaggcgagc agccaaggaa aggacgatga tttccccgac aacaccacgg aattgtcagt
     9481 gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga gttccgccgt
     9541 ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg tggcaatgcc
     9601 ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt gcctgacaac
     9661 gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg agaaaatgaa
     9721 agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc cacatagcgt
     9781 aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt aatccagagg
     9841 ttgattagga tctctcgagt tagcc
//
