LOCUS       sequence-325386-        9240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone (attL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Ren Q, Sretenovic S, Liu G, Zhong Z, Wang J, Huang L, Tang X, Guo Y,
            Liu L, Wu Y, Zhou J, Zhao Y, Yang H, He Y, Liu S, Yin D, Mayorga R, 
            Zheng X, Zhang T, Qi Y, Zhang Y
  TITLE     Improved plant cytosine base editors with high editing activity, 
            purity, and specificity.
  JOURNAL   Plant Biotechnol J. 2021 May 27. doi: 10.1111/pbi.13635.
  PUBMED    34042262
REFERENCE   2  (bases 1 to 9240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2021 May 27. doi: 10.1111/pbi.13635."
COMMENT     Sequence Label: pYPQ269C2
FEATURES             Location/Qualifiers
     source          1..9240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             358..1149
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1242..1830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1731..1750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1984..2001
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2160..2187)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2279..2365)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2414..2436
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2428..2445
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2429..2445
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2461..2560
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3215..3280
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3287..3307
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3332..3355,3356..3358,3359..7432)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             7345..7353
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             7433..7480
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7502..7750
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7763..7783
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7790..7843
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             7850..7870
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7898..8284
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             8333..8581
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8594..8614
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8621..8668
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      8690..8942
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    8987..9110
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(9127..9146)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9128..9146)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9151..9167)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9151..9167)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc cgggtgacgc
       61 acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
      121 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
      181 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
      241 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
      301 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacattatg
      361 agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
      421 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
      481 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
      541 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
      601 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
      661 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
      721 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
      781 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
      841 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
      901 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
      961 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     1021 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     1081 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     1141 ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta cgcgtcgttc
     1201 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     1261 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     1321 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     1381 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     1441 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     1501 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1561 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1621 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1681 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1741 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1801 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1861 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1921 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     1981 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2041 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2101 agtgagcgca acgcaattaa tacgcgtacc gcgagccagg aagagtttgt agaaacgcaa
     2161 aaaggccatc cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc
     2221 gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat
     2281 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt
     2341 ccgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgctt
     2401 gcatggatgt tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc
     2461 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
     2521 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgaattcgc ccttcatgga
     2581 ctcacttttg atgaataggc gggagttctt gtaccagttt aagaacgtgc ggtgggcgaa
     2641 aggtcggagg gaaacctacc tttgctatgt ggttaagaga cgcgacagcg caacatcctt
     2701 ctccttggac tttggatacc ttcgcaataa gaatgggtgt catgtcgaac tgttgtttct
     2761 ccggtatatt agcgattggg acctggaccc cggcaggtgt tacagggtga catggttcat
     2821 atcgtggtct ccatgctatg actgcgctag gcatgttgct gattttctta ggggcaatcc
     2881 aaacctgagt cttcgcattt tcacggcgcg gctgtatttt tgtgaggacc gcaaggcaga
     2941 gcccgaggga ttgcgcaggc ttcatagggc aggagtccaa atagctatca tgactttcaa
     3001 agactatttt tactgttgga acacgttcgt cgagaaccac ggacggacgt ttaaggcttg
     3061 ggaaggactg catgaaaaca gtgtcaggct ctcaaggcaa ctccgccgca tacttttgcc
     3121 tttgtacgag gttgacgacc tgcgcgacgc atttcggact tcaggttctg agacccctgg
     3181 cacaagtgag tcagcaacac ccgagtccac catggattac aaggaccacg acggggatta
     3241 caaggaccac gacattgatt acaaggatga tgatgacaag atggctccga agaagaagag
     3301 gaaggttggc atccacgggg tgccagctgc tgacaagaag tactcgatcg gcctcgctat
     3361 tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa
     3421 gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct
     3481 cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg
     3541 gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc
     3601 gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa
     3661 gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa
     3721 gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct
     3781 ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga
     3841 gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac
     3901 gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat
     3961 cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg
     4021 cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa
     4081 cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata
     4141 cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct
     4201 cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga
     4261 gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga
     4321 tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt
     4381 cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga
     4441 gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt
     4501 gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc
     4561 acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc
     4621 tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta
     4681 cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga
     4741 gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt
     4801 catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca
     4861 ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac
     4921 cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct
     4981 cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa
     5041 gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct
     5101 ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga
     5161 gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat
     5221 gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct
     5281 caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg
     5341 ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg
     5401 caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc
     5461 tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc
     5521 agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat
     5581 gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca
     5641 gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct
     5701 ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct
     5761 ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa
     5821 caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc
     5881 cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc
     5941 ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct
     6001 gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct
     6061 ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt
     6121 tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg
     6181 cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca
     6241 gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc
     6301 ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg
     6361 ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa
     6421 ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac
     6481 tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga
     6541 gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca
     6601 agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct
     6661 gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta
     6721 cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa
     6781 gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg
     6841 ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa
     6901 gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa
     6961 gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa
     7021 gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga
     7081 caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga
     7141 gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct
     7201 ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca
     7261 tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat
     7321 cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc
     7381 gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc
     7441 agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag ggagacggat caggcgggtc
     7501 aacaaacctc agtgacatca tagagaagga aactggtaag caactggtta ttcaagagag
     7561 cattctgatg ctccctgagg aagtcgagga agttatagga aacaagcctg agagcgatat
     7621 actcgtgcat acggcgtatg acgagagcac ggatgaaaat gtgatgctct tgaccagcga
     7681 tgcgccagaa tataaaccct gggcactggt tattcaggac tctaacgggg aaaataaaat
     7741 caagatgctc tcaggtggct ccccaaagaa gaaacgcaag gtcaattggg agggcagagg
     7801 aagtcttcta acatgcggtg acgtggagga gaatcccggc cctatggctc ctaagaagaa
     7861 gcggaaggtt ggtattcacg gggtgcctgc ggctatggcg tcaaatttca cgcagtttgt
     7921 tttggttgat aacggcggga ctggcgacgt tacagtagct ccatcaaatt ttgcgaacgg
     7981 agtcgctgag tggattagct caaattcaag gtcccaggcc tacaaggtta cctgttctgt
     8041 taggcagagt tctgcgcaaa aaagaaaata taccatcaag gttgaagtcc ctaaagttgc
     8101 aacacaaaca gtcggtggtg ttgagctccc tgtggcagcc tggagatctt acttaaacat
     8161 ggagctaaca attccaatat tcgctacaaa ctctgattgt gaactgattg ttaaggcgat
     8221 gcaaggtctc ttgaaagatg gaaaccctat accgtccgct atcgcagcta acagcggtat
     8281 ctatggctct ggatcggggt cgggttctgg ctcaactagt tcaggcgggt caacaaacct
     8341 cagtgacatc atagagaagg aaactggtaa gcaactggtt attcaagaga gcattctgat
     8401 gctccctgag gaagtcgagg aagttatagg aaacaagcct gagagcgata tactcgtgca
     8461 tacggcgtat gacgagagca cggatgaaaa tgtgatgctc ttgaccagcg atgcgccaga
     8521 atataaaccc tgggcactgg ttattcagga ctctaacggg gaaaataaaa tcaagatgct
     8581 ctcaggtggc tccccaaaga agaaacgcaa ggttggtacc aagcgtcctg ctgccaccaa
     8641 aaaggccgga caggctaaga aaaagaagta gtggcggccg ccgacgtccg atcgttcaaa
     8701 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     8761 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     8821 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     8881 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     8941 tcgggaattg atcccccctc gacagcttcc ggaaagggcg aattcgcaac tttgtataca
     9001 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat
     9061 aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg ccatccagct
     9121 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     9181 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgcctcctc
//
