LOCUS       sequence-325467-       25973 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  Conditional expression of 3 different fluorophores.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 25973)
  TITLE     Enhancer AAVs from the Allen Institute
REFERENCE   2  (bases 1 to 25973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AiP1076-Ai213 (TICL-EGFP-ICF-mOrange2-ICN-mKate2)
FEATURES             Location/Qualifiers
     source          1..25973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        32..79
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             98..901
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(161..180)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     771..790
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     930..952
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     944..961
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     945..961
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1105..1154
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     enhancer        4354..4657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          4937..5953
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     protein_bind    complement(6047..6080)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    6158..6206
                     /note="synthetic polyadenylation signal"
     polyA_signal    6215..6691
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    6703..7164
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     protein_bind    complement(7191..7224)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7234..7243
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(7240..7242,7243..7245,7246..7959)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7240..7242,7243..7245,7246..7956)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     7893..7914
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7984..8572
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8455..8466)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    8603..8810
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        11334..11637
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    13022..13055
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    13101..13149
                     /note="synthetic polyadenylation signal"
     polyA_signal    13158..13634
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    13713..13761
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    13921..13954
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      13966..13975
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13972..14679
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             14683..14709
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             14713..14739
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    14768..15356
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(15239..15250)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    15387..15594
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    15608..15641
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     misc_feature    15679..16267
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(16150..16161)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    16298..16505
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        19029..19332
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    20803..20851
                     /note="synthetic polyadenylation signal"
     polyA_signal    20860..21336
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     polyA_signal    21415..21463
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     regulatory      21668..21677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             21674..22369
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             22379..22435
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    22464..23052
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(22935..22946)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    23083..23290
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    23304..23337
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     promoter        23364..23863
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(23399..23422)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     23796..23815
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     protein_bind    24555..24604
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="attB site for the Mycopbacteriophage Bxb1 integrase 
                     (Xu et al., 2013)"
     rep_origin      complement(24917..25505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(24997..25016)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aacgttttgg cggtttaaac attaagcacg tgttgacaat taatcatcgg catagtatat
       61 cggcatagta taatacgaca aggtgaggaa ctaaaccatg ggatcggcca ttgaacaaga
      121 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
      181 acaacagacg atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
      241 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
      301 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
      361 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
      421 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
      481 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
      541 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
      601 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
      661 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
      721 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
      781 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
      841 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
      901 aagtgttaac tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
      961 tgaattcgag ctcggtaccg aaaaaccgct cagcggcgcc cgcacccaaa cgaaaacacc
     1021 cccggcccaa gcggaccggg ggtgtcgcgt gaaatggatc agtgagcgtg gccgtggtgg
     1081 tggccgtggc cgtgctcgtc ctcgtcggcc ggcttgtcga cgacggcggt ctccgtcgtc
     1141 aggatcatcc gggccaccga ggcggcgttg agaacagccg agcgggtcac cttggccgga
     1201 tcgacgacgc cggcggagac cagatcaccg aactccagcg tggcggcgtt gaagccctgc
     1261 cccttgggaa gttcggagat ttcggccggc caatcgattt taccggggtg gtgcccatcc
     1321 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1381 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     1441 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     1501 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     1561 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1621 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1681 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1741 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggtt
     1801 aattaagaat tcgagctcgc tagagcccca tcctcactga ctccgtcctg gagttggatg
     1861 agagataatg gccttacgtt gtgccagggg agggtcgggc tggatttagc aagatttacc
     1921 ttctccaaag agcggtgctg cagtggcaca gctgcccacg gaggtggggg ggtcaccgtc
     1981 cctggaggtg atgaagaact gtggggatgt ggcactgagg gacatggcca gtgggcacgg
     2041 tgggtgggtt ggggttggtc ttggggatct tggagggctt ttccagcctt catgatttga
     2101 cgattgtatg aacatctaca tggcaattct ccagctgcct gtcccagtcc tactgaccca
     2161 gctgtatctc tccaggcaag ctcttccacc ccttctgctt gcatccagac accatcaaac
     2221 atgcaggctc agacacaggg accagcagtg tctgtggcct ttttgtgctc ctctccatgc
     2281 tgggttttaa cttgctcttt gtccttctat cctatcttct tatccttaag gctgttctga
     2341 acgctgtgac ttggagagtg tcccagagcc ctcaacacct gcatgtccca cgtccatgct
     2401 gtcctgcact tccttatccc caagatctgc ctctccgtga tgcactgaat tggcaaacat
     2461 gtgtcacccc agaccaacaa tgtcacagca aactccccct tgataggaca agggggaatg
     2521 gctttacact gagacagggg aggtttgggt tggatatgag gaggcagttt ttcccccaga
     2581 gggtggtgac gcactgaaca ggttgcccaa ggaggctgtg gatgccccat ccctgcaggc
     2641 attcaaggcc aggctggatg tggctctggg cagcctgggc tgctggttga tgaccctgca
     2701 catagcaggg ggttggatct ggatgagcac tgtgctcctt tgcaacccag gccgttctat
     2761 gattctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat ccccccaggt
     2821 gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc
     2881 cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga gcgccggacc
     2941 ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg taattacatc
     3001 cctgggggct ttgggggggg gctgtccgcc ccatcctcac tgactccgtc ctggagttgg
     3061 atgagagata atggccttac gttgtgccag gggagggtcg ggctggattt agcaagattt
     3121 accttctcca aagagcggtg ctgcagtggc acagctgccc acggaggtgg gggggtcacc
     3181 gtccctggag gtgatgaaga actgtgggga tgtggcactg agggacatgg ccagtgggca
     3241 cggtgggtgg gttggggttg gtcttgggga tcttggaggg cttttccagc cttcatgatt
     3301 tgacgattgt atgaacatct acatggcaat tctccagctg cctgtcccag tcctactgac
     3361 ccagctgtat ctctccaggc aagctcttcc accccttctg cttgcatcca gacaccatca
     3421 aacatgcagg ctcagacaca gggaccagca gtgtctgtgg cctttttgtg ctcctctcca
     3481 tgctgggttt taacttgctc tttgtccttc tatcctatct tcttatcctt aaggctgttc
     3541 tgaacgctgt gacttggaga gtgtcccaga gccctcaaca cctgcatgtc ccacgtccat
     3601 gctgtcctgc acttccttat ccccaagatc tgcctctccg tgatgcactg aattggcaaa
     3661 catgtgtcac cccagaccaa caatgtcaca gcaaactccc ccttgatagg acaaggggga
     3721 atggctttac actgagacag gggaggtttg ggttggatat gaggaggcag tttttccccc
     3781 agagggtggt gacgcactga acaggttgcc caaggaggct gtggatgccc catccctgca
     3841 ggcattcaag gccaggctgg atgtggctct gggcagcctg ggctgctggt tgatgaccct
     3901 gcacatagca gggggttgga tctggatgag cactgtgctc ctttgcaacc caggccgttc
     3961 tatgattctg tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca
     4021 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
     4081 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
     4141 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
     4201 atccctgggg gctttggggg ggggctgtcc ctctaggggt acccggggat ccaccggtcc
     4261 ttaattaacc gtttaaacaa ttctgcagga atctagttat taatagtaat caattacggg
     4321 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     4381 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4441 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     4501 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     4561 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     4621 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
     4681 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
     4741 attattttgt gcagcgatgg gggcgggggg gggggggggg sgsgsscsgg gggggggggg
     4801 gggggggggg gggggggggg ggggggggsg grgrggkgsg gsggsrgcca mycrgrgsgg
     4861 cgcgcyccga aagtttcctt ttwkggcgrg gcggcggcgg cggcggccct ataaaaagcg
     4921 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
     4981 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
     5041 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
     5101 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
     5161 ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc
     5221 cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga
     5281 ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg
     5341 ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct
     5401 gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg
     5461 gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggkggg
     5521 ggtgccgggc ggggcggggc cgccycgggc cggggrgggs kcgggggrgg ggcgcggcgg
     5581 cccccggrgc gccggcggct gtcgrggcgc ggcgrgccgc mgccmttgcc ttttwtggka
     5641 atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg
     5701 gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa
     5761 ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca
     5821 gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca gggcggggtt
     5881 cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc atgccttctt
     5941 ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc attttggcaa
     6001 agaattgatt tgataccatt taaatgggga tcaaattcaa ttaaatataa cttcgtatag
     6061 catacattat acgaagttat tcgcgagtga ataaatgaaa gcttgcagat cccctcagca
     6121 ccatggctag cggcagcctc ggagtttgaa tagatagaat aaaatatctt tattttcatt
     6181 ccatctgtgt gttggttttt tgtgtgagat ctacgggtgg catccctgtg acccctcccc
     6241 agtgcctctc ctggccctgg aagttgccac tccagtgccc accagccttg tcctaataaa
     6301 attaagttgc atcattttgt ctgactaggt gtccttctat aatattatgg ggtggagggg
     6361 ggtggtatgg agcaaggggc aagttgggaa gacaacctgt agggcctgcg gggtctattg
     6421 ggaaccaagc tggagtgcag tggcacaatc ttggctcact gcaatctccg cctcctgggt
     6481 tcaagcgatt ctcctgcctc aggctcccga gttgttggga ttccaggcat gcatgaccag
     6541 gctcagctaa tttttgtttt tttggtagag acggggtttc accatattgg ccaggctggt
     6601 ctccaactcc taatctcagg tgatctaccc accttggcct cccaaattgc tgggattaca
     6661 ggcgtgaacc actgctccct tccctgtcct tctgcctcag cgctgtaagt ctgcagaaat
     6721 tgatgatcta ttaaacaata aagatgtcca ctaaaatgga agtttttcct gtcatacttt
     6781 gttaagaagg gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg
     6841 tgggggtggg gtgggattag ataaatgcct gctctttact gaaggctctt tactattgct
     6901 ttatgataat gtttcatagt tggatatcat aatttaaaca agcaaaacca aattaagggc
     6961 cagctcattc ctcccactca tgatctatag atctatagat ctctcgtggg atcattgttt
     7021 ttctcttgat tcccactttg tggttctaag tactgtggtt tccaaatgtg tcagtttcat
     7081 agcctgaaga acgagatcag cagcctctgt tccacataca cttcattctc agtattgttt
     7141 tgccaagttc taattccatc agaaagcttt tacttaactg aatagctaac ataacttcgt
     7201 atagcataca ttatacgaag ttatcacgcg tccgccacca tggtgagcaa gggcgaggag
     7261 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     7321 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     7381 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     7441 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     7501 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     7561 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     7621 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     7681 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     7741 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     7801 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     7861 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     7921 gccgggatca ctctcggcat ggacgagctg tacaagtaaa cgcgttacgt aaagcttgtc
     7981 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     8041 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     8101 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     8161 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     8221 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     8281 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     8341 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     8401 ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     8461 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     8521 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgacac
     8581 cgagcgctgc tcgaggcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     8641 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     8701 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     8761 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga cacgtgggat
     8821 cctctagagc cccatcctca ctgactccgt cctggagttg gatgagagat aatggcctta
     8881 cgttgtgcca ggggagggtc gggctggatt tagcaagatt taccttctcc aaagagcggt
     8941 gctgcagtgg cacagctgcc cacggaggtg ggggggtcac cgtccctgga ggtgatgaag
     9001 aactgtgggg atgtggcact gagggacatg gccagtgggc acggtgggtg ggttggggtt
     9061 ggtcttgggg atcttggagg gcttttccag ccttcatgat ttgacgattg tatgaacatc
     9121 tacatggcaa ttctccagct gcctgtccca gtcctactga cccagctgta tctctccagg
     9181 caagctcttc caccccttct gcttgcatcc agacaccatc aaacatgcag gctcagacac
     9241 agggaccagc agtgtctgtg gcctttttgt gctcctctcc atgctgggtt ttaacttgct
     9301 ctttgtcctt ctatcctatc ttcttatcct taaggctgtt ctgaacgctg tgacttggag
     9361 agtgtcccag agccctcaac acctgcatgt cccacgtcca tgctgtcctg cacttcctta
     9421 tccccaagat ctgcctctcc gtgatgcact gaattggcaa acatgtgtca ccccagacca
     9481 acaatgtcac agcaaactcc cccttgatag gacaaggggg aatggcttta cactgagaca
     9541 ggggaggttt gggttggata tgaggaggca gtttttcccc cagagggtgg tgacgcactg
     9601 aacaggttgc ccaaggaggc tgtggatgcc ccatccctgc aggcattcaa ggccaggctg
     9661 gatgtggctc tgggcagcct gggctgctgg ttgatgaccc tgcacatagc agggggttgg
     9721 atctggatga gcactgtgct cctttgcaac ccaggccgtt ctatgattct gtcattctaa
     9781 atctctcttt cagcctaaag ctttttcccc gtatcccccc aggtgtctgc aggctcaaag
     9841 agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt
     9901 ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg
     9961 cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg
    10021 gggggctgtc cctctagagc cccatcctca ctgactccgt cctggagttg gatgagagat
    10081 aatggcctta cgttgtgcca ggggagggtc gggctggatt tagcaagatt taccttctcc
    10141 aaagagcggt gctgcagtgg cacagctgcc cacggaggtg ggggggtcac cgtccctgga
    10201 ggtgatgaag aactgtgggg atgtggcact gagggacatg gccagtgggc acggtgggtg
    10261 ggttggggtt ggtcttgggg atcttggagg gcttttccag ccttcatgat ttgacgattg
    10321 tatgaacatc tacatggcaa ttctccagct gcctgtccca gtcctactga cccagctgta
    10381 tctctccagg caagctcttc caccccttct gcttgcatcc agacaccatc aaacatgcag
    10441 gctcagacac agggaccagc agtgtctgtg gcctttttgt gctcctctcc atgctgggtt
    10501 ttaacttgct ctttgtcctt ctatcctatc ttcttatcct taaggctgtt ctgaacgctg
    10561 tgacttggag agtgtcccag agccctcaac acctgcatgt cccacgtcca tgctgtcctg
    10621 cacttcctta tccccaagat ctgcctctcc gtgatgcact gaattggcaa acatgtgtca
    10681 ccccagacca acaatgtcac agcaaactcc cccttgatag gacaaggggg aatggcttta
    10741 cactgagaca ggggaggttt gggttggata tgaggaggca gtttttcccc cagagggtgg
    10801 tgacgcactg aacaggttgc ccaaggaggc tgtggatgcc ccatccctgc aggcattcaa
    10861 ggccaggctg gatgtggctc tgggcagcct gggctgctgg ttgatgaccc tgcacatagc
    10921 agggggttgg atctggatga gcactgtgct cctttgcaac ccaggccgtt ctatgattct
    10981 gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc aggtgtctgc
    11041 aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg
    11101 cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg
    11161 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg
    11221 ggctttgggg gggggctgtc cctctagagt cgacggcgcg ccctgcagga atctagttat
    11281 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
    11341 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
    11401 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
    11461 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
    11521 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
    11581 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
    11641 gaggtgagcc ccmcgttctg cttcmcycyc cccmycyccc cccccycccc mcccccaatt
    11701 ttktwtttwt ttwttttttw wttwtttkgk gsrgsgrkgg gggsgggggg gggggggggg
    11761 cgcgcgcsrg gsggggsggg gcggggcgrg gggcggggcg gggcgaggcg gagaggtgcg
    11821 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
    11881 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggna gtcgctgcgc gctgccttcg
    11941 ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt
    12001 actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt
    12061 ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg
    12121 ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc
    12181 cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg
    12241 tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg
    12301 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
    12361 tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca
    12421 cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg
    12481 cggggggtgg cggcaggkgg gggtgccggg cggggcgggg ccgccycggg ccggggrggg
    12541 skcgggggrg gggsgcggcg gcccccggrg cgccggcggc ygtcgrggcg cggcgrgccg
    12601 cmgccmttgc cttttwtggt aatcgtgcgr gagggcgcag ggacttcctt tgtcccaaat
    12661 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
    12721 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
    12781 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
    12841 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
    12901 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
    12961 gctgtctcat cattttggca aagaattgat ttgataccgc gggccccgcg tggacggtac
    13021 cgaagttcct attctctaga aagtatagga acttccctca gcaccatggc tagcggcagc
    13081 ctcggagttt gaatagatag aataaaatat ctttattttc attccatctg tgtgttggtt
    13141 ttttgtgtga gatctacggg tggcatccct gtgacccctc cccagtgcct ctcctggccc
    13201 tggaagttgc cactccagtg cccaccagcc ttgtcctaat aaaattaagt tgcatcattt
    13261 tgtctgacta ggtgtccttc tataatatta tggggtggag gggggtggta tggagcaagg
    13321 ggcaagttgg gaagacaacc tgtagggcct gcggggtcta ttgggaacca agctggagtg
    13381 cagtggcaca atcttggctc actgcaatct ccgcctcctg ggttcaagcg attctcctgc
    13441 ctcaggctcc cgagttgttg ggattccagg catgcatgac caggctcagc taatttttgt
    13501 ttttttggta gagacggggt ttcaccatat tggccaggct ggtctccaac tcctaatctc
    13561 aggtgatcta cccaccttgg cctcccaaat tgctgggatt acaggcgtga accactgctc
    13621 ccttccctgt ccttctgcct cagctaattg agtagggggg aggctaactg aaacacggaa
    13681 ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca
    13741 cgggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
    13801 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
    13861 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccgtg
    13921 gaagttccta ttctctagaa agtataggaa cttcacgcgc gcgccgccac catggtgagc
    13981 aagggcgagg agaataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcgcatg
    14041 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
    14101 gagggctttc agaccgctaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
    14161 gacatcctgt cccctcattt cacctacggc tccaaggcct acgtgaagca ccccgccgac
    14221 atccccgact acttcaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
    14281 tacgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
    14341 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt gatgcagaag
    14401 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg tgccctgaag
    14461 ggcaagatca agatgaggct gaagctgaag gacggcggcc actacacctc cgaggtcaag
    14521 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt cgacatcaag
    14581 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
    14641 ggccgccact ccaccggcgg catggacgag ctgtacaagg ggtatcccta tgatgtacca
    14701 gactatgccg ggtatcccta tgatgtacca gactatgcct aacaattgtt acgtaaagct
    14761 tgtcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
    14821 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
    14881 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
    14941 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
    15001 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
    15061 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
    15121 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
    15181 gctgctcgcc tatgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
    15241 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
    15301 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
    15361 acaccgagcg ctgctcgagg cctcgactgt gccttctagt tgccagccat ctgttgtttg
    15421 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    15481 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    15541 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggacacgtg
    15601 ggatccggtg ccagggcgtg cccttgggct ccccgggcgc gtttgggcgg ccgcacgcgt
    15661 tacgtaaagc ttgtcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
    15721 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
    15781 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
    15841 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
    15901 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
    15961 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
    16021 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
    16081 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
    16141 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
    16201 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    16261 tccccgcatc gacaccgagc gctgctcgag gcctcgactg tgccttctag ttgccagcca
    16321 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
    16381 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
    16441 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
    16501 ggggacacgt gggatcctct agagccccat cctcactgac tccgtcctgg agttggatga
    16561 gagataatgg ccttacgttg tgccagggga gggtcgggct ggatttagca agatttacct
    16621 tctccaaaga gcggtgctgc agtggcacag ctgcccacgg aggtgggggg gtcaccgtcc
    16681 ctggaggtga tgaagaactg tggggatgtg gcactgaggg acatggccag tgggcacggt
    16741 gggtgggttg gggttggtct tggggatctt ggagggcttt tccagccttc atgatttgac
    16801 gattgtatga acatctacat ggcaattctc cagctgcctg tcccagtcct actgacccag
    16861 ctgtatctct ccaggcaagc tcttccaccc cttctgcttg catccagaca ccatcaaaca
    16921 tgcaggctca gacacaggga ccagcagtgt ctgtggcctt tttgtgctcc tctccatgct
    16981 gggttttaac ttgctctttg tccttctatc ctatcttctt atccttaagg ctgttctgaa
    17041 cgctgtgact tggagagtgt cccagagccc tcaacacctg catgtcccac gtccatgctg
    17101 tcctgcactt ccttatcccc aagatctgcc tctccgtgat gcactgaatt ggcaaacatg
    17161 tgtcacccca gaccaacaat gtcacagcaa actccccctt gataggacaa gggggaatgg
    17221 ctttacactg agacagggga ggtttgggtt ggatatgagg aggcagtttt tcccccagag
    17281 ggtggtgacg cactgaacag gttgcccaag gaggctgtgg atgccccatc cctgcaggca
    17341 ttcaaggcca ggctggatgt ggctctgggc agcctgggct gctggttgat gaccctgcac
    17401 atagcagggg gttggatctg gatgagcact gtgctccttt gcaacccagg ccgttctatg
    17461 attctgtcat tctaaatctc tctttcagcc taaagctttt tccccgtatc cccccaggtg
    17521 tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc
    17581 ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag cgccggaccg
    17641 gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt aattacatcc
    17701 ctgggggctt tggggggggg ctgtccctct agagccccat cctcactgac tccgtcctgg
    17761 agttggatga gagataatgg ccttacgttg tgccagggga gggtcgggct ggatttagca
    17821 agatttacct tctccaaaga gcggtgctgc agtggcacag ctgcccacgg aggtgggggg
    17881 gtcaccgtcc ctggaggtga tgaagaactg tggggatgtg gcactgaggg acatggccag
    17941 tgggcacggt gggtgggttg gggttggtct tggggatctt ggagggcttt tccagccttc
    18001 atgatttgac gattgtatga acatctacat ggcaattctc cagctgcctg tcccagtcct
    18061 actgacccag ctgtatctct ccaggcaagc tcttccaccc cttctgcttg catccagaca
    18121 ccatcaaaca tgcaggctca gacacaggga ccagcagtgt ctgtggcctt tttgtgctcc
    18181 tctccatgct gggttttaac ttgctctttg tccttctatc ctatcttctt atccttaagg
    18241 ctgttctgaa cgctgtgact tggagagtgt cccagagccc tcaacacctg catgtcccac
    18301 gtccatgctg tcctgcactt ccttatcccc aagatctgcc tctccgtgat gcactgaatt
    18361 ggcaaacatg tgtcacccca gaccaacaat gtcacagcaa actccccctt gataggacaa
    18421 gggggaatgg ctttacactg agacagggga ggtttgggtt ggatatgagg aggcagtttt
    18481 tcccccagag ggtggtgacg cactgaacag gttgcccaag gaggctgtgg atgccccatc
    18541 cctgcaggca ttcaaggcca ggctggatgt ggctctgggc agcctgggct gctggttgat
    18601 gaccctgcac atagcagggg gttggatctg gatgagcact gtgctccttt gcaacccagg
    18661 ccgttctatg attctgtcat tctaaatctc tctttcagcc taaagctttt tccccgtatc
    18721 cccccaggtg tctgcaggct caaagagcag cgagaagcgt tcagaggaaa gcgatcccgt
    18781 gccaccttcc ccgtgcccgg gctgtccccg cacgctgccg gctcggggat gcggggggag
    18841 cgccggaccg gagcggagcc ccgggcggct cgctgctgcc ccctagcggg ggagggacgt
    18901 aattacatcc ctgggggctt tggggggggg ctgtccctct agagtcgacg gcgcgccctg
    18961 caggaatcta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
    19021 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
    19081 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
    19141 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
    19201 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
    19261 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
    19321 gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat ctcccccccc
    19381 tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg
    19441 gggggggggg ggggnsgsgc sccrggsggg gsgggggggg gggggggggg gggggggggg
    19501 gggggggggg gggsggsggs rgscmvhmmg rgsggsgsgs yccsrrrgkt ycyttttwkg
    19561 gsgrggsggs ggsggsggcg gcccyataaa aagcgargcg cgcggcgggs ggggrgtcgc
    19621 tgcgmcgctg cctycgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
    19681 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
    19741 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
    19801 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
    19861 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
    19921 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
    19981 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
    20041 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
    20101 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
    20161 ggctcgccgt gccgggcggg gggtggcggc aggkgggggt gccgggcggg gcggggccgc
    20221 cycgggccgg ggrgggskcg ggggrggggc gcggcggccc ccggrgcgcc ggcggcygtc
    20281 grggcgcggc grgccgcmgc cmttgccttt twtggkaatc gtgcgagagg gcgcagggac
    20341 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
    20401 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
    20461 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
    20521 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
    20581 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
    20641 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attgatttga taccgcgggc
    20701 cccgcgtgga cgttataagt acctgaatgt cctataatta cacttatagg acattcacct
    20761 cagcaccatg gctagcggca gcctcggagt ttgaatagat agaataaaat atctttattt
    20821 tcattccatc tgtgtgttgg ttttttgtgt gagatctacg ggtggcatcc ctgtgacccc
    20881 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
    20941 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
    21001 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
    21061 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
    21121 tgggttcaag cgattctcct gcctcaggct cccgagttgt tgggattcca ggcatgcatg
    21181 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
    21241 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
    21301 ttacaggcgt gaaccactgc tcccttccct gtccttctgc ctcagctaat tgagtagggg
    21361 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa
    21421 taaaaagaca gaataaaacg cacgggtgtt gggtcgtttg ttcataaacg cggggttcgg
    21481 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc
    21541 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc
    21601 aacgtcgggg cggcaggccg tgtgaatgtc ctataattac acttatagga cattcaacgc
    21661 gcgcgccgcc accatggtga gcgagctgat taaggagaac atgcacatga agctgtacat
    21721 ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag gcaagcccta
    21781 cgagggcacc cagaccatga gaatcaaggc ggtcgagggc ggccctctcc ccttcgcctt
    21841 cgacatcctg gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg
    21901 catccccgac ttctttaagc agtccttccc cgagggcttc acatgggaga gagtcaccac
    21961 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
    22021 catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa
    22081 gaaaacactc ggctgggagg cctccaccga gaccctgtac cccgctgacg gcggcctgga
    22141 aggcagagcc gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttgaa
    22201 gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt
    22261 ggacagaaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg agcagcacga
    22321 ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacagag gaagcggagc
    22381 cactaacttc tccctgttga aacaagcagg ggatgtcgaa gagaatcccg ggccatgaca
    22441 attgttacgt aaagcttgtc gataatcaac ctctggatta caaaatttgt gaaagattga
    22501 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
    22561 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
    22621 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
    22681 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
    22741 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
    22801 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
    22861 catcgtcctt tccttggctg ctcgcctatg ttgccacctg gattctgcgc gggacgtcct
    22921 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
    22981 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
    23041 ccgcctcccc gcatcgacac cgagcgctgc tcgaggcctc gactgtgcct tctagttgcc
    23101 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
    23161 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
    23221 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
    23281 atgctgggga cacgtgggat ccggtgccag ggcgtgccct tgggctcccc gggcgcgttt
    23341 gggcggccgc atcgaattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc
    23401 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac
    23461 acattccaca tcctccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc
    23521 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
    23581 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
    23641 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
    23701 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
    23761 cggggcgggc gcccgaaggk cctccggrgg cccggcattc tgcacgcttc aaaagcgcac
    23821 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca atcgccgcta
    23881 gccgccacca tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
    23941 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
    24001 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
    24061 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
    24121 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
    24181 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
    24241 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
    24301 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
    24361 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgtaagtgc tagcccctag
    24421 gcctgaatcg aaaaaccgct cagcggcgcc cgcacccaaa cgaaaacacc cccggcccaa
    24481 gcggaccggg ggtgtcgcgt gaaatggatc agtgagcgtg gccgtggtgg tggccgtggc
    24541 cgtgctcgtc ctcgtcggcc ggcttgtcga cgacggcggt ctccgtcgtc aggatcatcc
    24601 gggccaccga ggcggcgttg agaacagccg agcgggtcac cttggccgga tcgacgacgc
    24661 cggcggagac cagatcaccg aactccagcg tggcggcgtt gaagccctgc cccttgggaa
    24721 gttcggagag agctcgtcga ctgcagaggc ctgcatgcaa gcttggcgta atcatggtca
    24781 tagctgtttc cttatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    24841 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    24901 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    24961 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    25021 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    25081 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    25141 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    25201 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    25261 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    25321 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    25381 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    25441 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    25501 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    25561 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    25621 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    25681 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    25741 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    25801 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    25861 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    25921 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgc
//
