LOCUS       sequence-325506-        6110 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of TPMV F glycoprotein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Argaw T, Marino MP, Timmons A, Eldridge L, Takeda K, Li P, Kwilas A,
            Ou W, Reiser J
  TITLE     In vivo targeting of lentiviral vectors pseudotyped with the Tupaia 
            paramyxovirus H glycoprotein bearing a cell-specific ligand.
  JOURNAL   Mol Ther Methods Clin Dev. 2021 Apr 24;21:670-680. doi: 
            10.1016/j.omtm.2021.04.012. eCollection 2021 Jun 11.
  PUBMED    34141822
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2021-04-24"; volume: "21:670-680. doi"; 
            pages: " 10.1016/j.omtm.2021.04.012. eCollection 2021 Jun 11"
COMMENT     Sequence Label: pCG-TPMV Fd32
FEATURES             Location/Qualifiers
     source          1..6110
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          325..898
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     906..925
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        953..971
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1010..1019
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2771..2790)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2836..2891
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2890..2909)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3245..3380
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3486..3503)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3657..4245)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3737..3756)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4416..5207,5208..5276))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5039..5058
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5277..5381)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5449..5467
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5505..5527)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5627..5646
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5765..5783
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        5807..6110
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg ggggatcgat
      301 ccccccgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc tttctttttc
      361 gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt gtttagaatg
      421 ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt tcactttcta
      481 ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac tttttcgtta
      541 aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt gtcagattgt
      601 aagtactttc tctaatcact ttttttttca aggcaatcag ggtatattat attgtacttc
      661 agcacagttt tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat
      721 aaattctggc tggcgtggaa atattcttat tggtagaaac aactacatcc tggtcatcat
      781 cctgcctttc tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc
      841 tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
      901 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tgtaatacga
      961 ctcactatag ggcgaattcg gatcccccgg tagttaatta aaaattgccg ccaccatggc
     1021 atctctgctg aagaccatct gctacatcta tctgattaca tacgccaaac tggagcccac
     1081 acccaagtca cagctggacc tggacagcct ggcatccatc ggagtggtcg acgccggaaa
     1141 gtacaactat aaactgatga ccacaggctc cgagaagctg atggtcatca agctggtccc
     1201 aaatattaca tacgccacta attgcaacct gaccgcccac acagcttata ctaagatgat
     1261 cgaacggctg ctgaccccca ttaaccagtc tctgtacgag atgaggagtg tgatcacaga
     1321 acgcgatggc gggactattt tctggggagc aatcattgca ggagccgctc tgggagtggc
     1381 aacagcagcc gctatcactg ctggagtcgc actgcaccga gcagagcaga atgcccggaa
     1441 catcgcagcc ctgaaggacg ctctgagaaa ttctaacgaa gcaattcagc acctgaaaga
     1501 tgcccaggga catacagtgc tggctatcca gggcctgcag gagcagatta acaacaacat
     1561 catccctaag ctgaaagaaa gccattgtct gggcgtgaat aaccagctgg ggctgctgct
     1621 gaaccagtac tattccgaga tcctgaccgt gtttggccct aatctgcaga acccagtctc
     1681 cgcctctctg acaatccagg ccattgctaa ggcattcaat ggggacttta acagcctgat
     1741 gactaatctg aactacgacc caaccgatct gctggacatc ctggagagta attcaattaa
     1801 cggccgcatc attgacgtga atctgaacga gaagtatatc gcactgtcta tcgaaattcc
     1861 aaacttcatt actctgaccg atgctaagat ccagaccttt aatcgaatca catacgggta
     1921 tggaagtaac gagtggctga ccctgatccc cgacaatatt ctggaatacg gaaatctgat
     1981 ctcaaacgtg gatctgacta gctgcgtcaa gaccaaaagc tcctacatct gtaatcagga
     2041 cacaagctat cctatttcta gtgagctgac tcggtgcctg agaggcgata cctcaagctg
     2101 tccaagaaca cccgtggtca acagtagggc tccaactttc gccttaagtg gaggccatat
     2161 ctatgccaat tgcgctaagg ctgcatgcag gtgtgagaaa ccccctatgg ccattgtgca
     2221 gcccgctaca agcactctga cctttctgac agagaaggaa tgtcaggaag tggtcatcga
     2281 ccagatcaac attcagctgg cccctaatcg cctgaacaaa accatcatta cagatggaat
     2341 cgacctgggc cccgaagtga tcatcaaccc tatcgatgtc tccgccgaac tggggaatat
     2401 tgagctggaa atggataaga cccagaaagc tctggacagg tccaataaga tcctggattc
     2461 tatgattact gaggtgaccc ccgataaact gctgatcgcc atgattgtgg tcttcggcat
     2521 cctgctgctg tggctgttcg gggtctccta ttacgcattc aaaatctggt ccaaactgtg
     2581 acgactagtg tgaaatagac atcagaatta agaaaaacgt agggtccaag tggttccccg
     2641 ttatggactc gctatctgtc aaccagatct tataccctga agttcaccta gatagcccga
     2701 tagttaccaa taagatagta gccatcctgg agtatgctcg actgtttaaa cctgcaggca
     2761 tgcaagctga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     2821 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     2881 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     2941 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     3001 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     3061 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     3121 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     3181 tcccagtcat agctgtccct cttctcttat ggagatccct cgaggagctt tttgcaaaag
     3241 cctaggcctc caaaaaagcc tcttcactac ttctggaata gctcagaggc cgaggcggcc
     3301 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     3361 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     3421 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     3481 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     3541 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     3601 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     3661 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     3721 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3781 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3841 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3901 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3961 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     4021 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     4081 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     4141 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     4201 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     4261 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     4321 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4381 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4441 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     4501 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     4561 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     4621 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     4681 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     4741 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4801 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4861 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4921 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4981 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5041 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5101 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5161 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5221 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5281 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     5341 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     5401 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     5461 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     5521 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     5581 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     5641 attgtactga gagtgcacca ttcgacgctc tcccttatgc gactcctgca ttaggaagca
     5701 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtc tggcttatcg
     5761 aaattaatac gactcactat agggagaccg gaattcgagc tcgccccgtt acataactta
     5821 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     5881 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     5941 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     6001 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     6061 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
