LOCUS       sequence-32579-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Semple JI, Garcia-Verdugo R, Lehner B
  TITLE     Rapid selection of transgenic C. elegans using antibiotic 
            resistance.
  JOURNAL   Nat Methods. 2010 Sep;7(9):725-7. doi: 10.1038/nmeth.1495. Epub 2010
            Aug 22.
  PUBMED    20729840
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1495"; journalName: "Nat Methods"; date: "2010-09"; 
            volume: "7"; issue: "9"; pages: "725-7"
COMMENT     Sequence Label: pBCN27-R4R3
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(86..92,93..110,111..116))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(141..264)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(272..289)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(272..288)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
       61 gacggatcct aactcaaaat ccacacatta tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
      181 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      241 gttcaacttt attatacata gttgataatt cactggccgt cgttttacac tcgaggggag
      301 aaaatgcgga gagaagaacc cagcatctta ataaaattat ataattttga ataaaatcaa
      361 attaaaaata agattaattg aaacacaaca atagaatgca gaaaattact cataagaaat
      421 aatttatttt gaaagaagtt gtgtcgattt acgataaaaa ctacacgcaa acgaggctcc
      481 gcgtttacac cgtttggcaa tggagcgcgc ttgctattag attttaggcc agctcaacga
      541 aagtttaatt tatttttctt ctttttttcc atttttttga gtaaaatatc gaattttttg
      601 tattaattct gtaaatttta taatttgcaa ctattttggc gtattctgct tgtttttatc
      661 agcgaatttt tatgcatttc agtcaaaagt ttgagcagga aaaatgacat ttaggacttt
      721 gtaaaattgt attcttaaat ttgcttgaaa acctacgtgt acaattgatt ttattgaatt
      781 tttcttacgt ttcccgttgt atcttattgt atttaacttt catcataaaa taatttattt
      841 ccaggtaaaa tatccgacgc tctcgtg
//
