LOCUS       sequence-325870-        5876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBRBAD-rbsD-RFP-B0015- B0015-LasR-rbs34-J23108-pBRlasI-rbsD-RFP- 
            B0015.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Jayaraman P, Yeoh JW, Zhang J, Poh CL
  TITLE     Programming the Dynamic Control of Bacterial Gene Expression with a 
            Chimeric Ligand- and Light-Based Promoter System.
  JOURNAL   ACS Synth Biol. 2018 Nov 16;7(11):2627-2639. doi: 
            10.1021/acssynbio.8b00280. Epub 2018 Nov 6.
  PUBMED    30359530
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.8b00280"; journalName: "ACS Synth Biol"; date: 
            "2018-11-16- 16"; volume: "7"; issue: "11"; pages: "2627-2639"
COMMENT     Sequence Label: pRPLC
FEATURES             Location/Qualifiers
     source          1..5876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             35..46
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(797..843)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      complement(986..1013)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(1029..1100)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(1133..1810)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(1918..1937)
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2171..3049
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             3192..3986
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3246..3265)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3856..3875
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      4017..4111
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      4199..4787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4688..4707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(4945..4972)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(4988..5059)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5092..5769)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     promoter        join(5876,1..34)
                     /label=BBa_J23108
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.51"
ORIGIN
        1 tgacagctag ctcagtccta ggtataatgc tagcaaagag gagaaatact agagatggcc
       61 ttggttgacg gttttcttga gctggaacgc tcaagtggaa aattggagtg gagcgccatc
      121 ctccagaaga tggcgagcga ccttggattc tcgaagatcc tgttcggcct gttgcctaag
      181 gacagccagg actacgagaa cgccttcatc gtcggcaact acccggccgc ctggcgcgag
      241 cattacgacc gggctggcta cgcgcgggtc gacccgacgg tcagtcactg tacccagagc
      301 gtactgccga ttttctggga accgtccatc taccagacgc gaaagcagca cgagttcttc
      361 gaggaagcct cggccgccgg cctggtgtat gggctgacca tgccgctgca tggtgctcgc
      421 ggcgaactcg gcgcgctgag cctcagcgtg gaagcggaaa accgggccga ggccaaccgt
      481 ttcatagagt cggtcctgcc gaccctgtgg atgctcaagg actacgcact gcaaagcggt
      541 gccggactgg ccttcgaaca tccggtcagc aaaccggtgg ttctgaccag ccgggagaag
      601 gaagtgttgc agtggtgcgc catcggcaag accagttggg agatatcggt tatctgcaac
      661 tgctcggaag ccaatgtgaa cttccatatg ggaaatattc ggcggaagtt cggtgtgacc
      721 tcccgccgcg tagcggccat tatggccgtt aatttgggtc ttattactct ctaataatac
      781 tagagccagg catcaaataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      841 ttgatgcctg gagatcctta ctcgagtttg gatccttaag caccggtgga gtgacgacct
      901 tcagcacgtt cgtactgttc aacgatggtg tagtcttcgt tgtgagstga tatcsckcgc
      961 cgcagccgaa cgccctaggt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga
     1021 ctctagtaga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
     1081 tgagcctttc gttttatttg atgcctggag atccttactc gagtttggat ccttaagcac
     1141 cggtggagtg acgaccttca gcacgttcgt actgttcaac gatggtgtag tcttcgttgt
     1201 gggaggtgat gtccagtttg atgtcggttt tgtaagcacc cggcagctga accggttttt
     1261 tagccatgta ggtggtttta acttcagcgt cgtagtgacc accgtctttc agtttcagac
     1321 gcattttgat ttcacctttc agagcaccgt cttccgggta catacgttcg gtggaagctt
     1381 cccaacccat ggtttttttc tgcataaccg gaccgtcgga cgggaagttg gtaccacgca
     1441 gtttaacttt gtagatgaac tcaccgtctt gcagggagga gtcctgggta acggtaacaa
     1501 caccaccgtc ttcgaagttc ataacacgtt cccatttgaa accttccggg aaggacagtt
     1561 tcaggtagtc cgggatgtca gccgggtgtt taacgtaagc tttggaaccg tactggaact
     1621 gcggggacag gatgtcccaa gcgaacggca gcggaccacc tttggtaact ttcagtttag
     1681 cggtctgggt accttcgtac ggacgacctt caccttcacc ttcgatttcg aactcgtgac
     1741 cgttaacgga accttccata cgaactttga aacgcatgaa ctctttgata acgtcttcgc
     1801 tactcgccat atgtatatct ccttcttaaa agatcttttg aattcccaaa aaaacgggta
     1861 tggagaaaca gtacatggac taaaggctac ccgtcaggta gaatccgcta atcttatgga
     1921 taaaaatgct atggcatagc aaagtgtgac gccgtgcaaa taatcaatgt ggacttttct
     1981 gccgtgatta tagacacttt tgttacgcgt ttttgtcatg gctttggtcc cgctttgtta
     2041 cagaatgctt ttaataagcg gggttaccgg tttggttagc gagaagagcc agtaaaagac
     2101 gcagtgacgg caatgtctga tgcaatatgg acaattggtt tcttctctga atggcgggag
     2161 tatgaaaagt atggctgaag cgcaaaatga tcccctgctg ccgggatact cgtttaatgc
     2221 ccatctggtg gcgggtttaa cgccgattga ggccaacggt tatctcgatt tttttatcga
     2281 ccgaccgctg ggaatgaaag gttatattct caatctcacc attcgcggtc agggggtggt
     2341 gaaaaatcag ggacgagaat ttgtttgccg accgggtgat attttgctgt tcccgccagg
     2401 agagattcat cactacggtc gtcatccgga ggctcgcgaa tggtatcacc agtgggttta
     2461 ctttcgtccg cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat ttgccaatac
     2521 ggggttcttt cgcccggatg aagcgcacca gccgcatttc agcgacctgt ttgggcaaat
     2581 cattaacgcc gggcaagggg aagggcgcta ttcggagctg ctggcgataa atctgcttga
     2641 gcaattgtta ctgcggcgca tggaagcgat taacgagtcg ctccatccac cgatggataa
     2701 tcgggtacgc gaggcttgtc agtacatcag cgatcacctg gcagacagca attttgatat
     2761 cgccagcgtc gcacagcatg tttgcttgtc gccgtcgcgt ctgtcacatc ttttccgcca
     2821 gcagttaggg attagcgtct taagctggcg cgaggaccaa cgtatcagcc aggcgaagct
     2881 gcttttgagc accacccgga tgcctatcgc caccgtcggt cgcaatgttg gttttgacga
     2941 tcaactctat ttctcgcggg tatttaaaaa atgcaccggg gccagcccga gcgagttccg
     3001 tgccggttgt gaagaaaaag tgaatgatgt agccgtcaag ttgtcataag acgtcggaat
     3061 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     3121 tcttgccgcc aaggatctga tggcgcaggg gatcatgatc tgatcaagag acaggatgag
     3181 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     3241 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     3301 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     3361 tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     3421 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     3481 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     3541 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     3601 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     3661 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc
     3721 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     3781 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     3841 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     3901 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     3961 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgagagct cgcttggact
     4021 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
     4081 ttgccgccgg gcgtttttta ttggtgagaa tccaagcact agtcatgacc aaaatccctt
     4141 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4201 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4261 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4321 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4381 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4441 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4501 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4561 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4621 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4681 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4741 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4801 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4861 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4921 gcagccgaac gccctaggta taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac
     4981 tctagtagag agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact
     5041 gagcctttcg ttttatttga tgcctggaga tccttactcg agtttggatc cttaagcacc
     5101 ggtggagtga cgaccttcag cacgttcgta ctgttcaacg atggtgtagt cttcgttgtg
     5161 ggaggtgatg tccagtttga tgtcggtttt gtaagcaccc ggcagctgaa ccggtttttt
     5221 agccatgtag gtggttttaa cttcagcgtc gtagtgacca ccgtctttca gtttcagacg
     5281 cattttgatt tcacctttca gagcaccgtc ttccgggtac atacgttcgg tggaagcttc
     5341 ccaacccatg gtttttttct gcataaccgg accgtcggac gggaagttgg taccacgcag
     5401 tttaactttg tagatgaact caccgtcttg cagggaggag tcctgggtaa cggtaacaac
     5461 accaccgtct tcgaagttca taacacgttc ccatttgaaa ccttccggga aggacagttt
     5521 caggtagtcc gggatgtcag ccgggtgttt aacgtaagct ttggaaccgt actggaactg
     5581 cggggacagg atgtcccaag cgaacggcag cggaccacct ttggtaactt tcagtttagc
     5641 ggtctgggta ccttcgtacg gacgaccttc accttcacct tcgatttcga actcgtgacc
     5701 gttaacggaa ccttccatac gaactttgaa acgcatgaac tctttgataa cgtcttcgct
     5761 actcgccata tgtatatctc cttcttaaac atggactaaa ggctacctga agaatttatg
     5821 caaatttcat aattttataa ctagcaaatg agatagattt cggtgaacca gatccc
//
