LOCUS       sequence-32594-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin 
            JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM
  TITLE     Modification of superoxide dismutase 1 (SOD1) properties by a GFP 
            tag--implications for research into amyotrophic lateral sclerosis 
            (ALS).
  JOURNAL   PLoS One. 2010 . 5(3):e9541.
  PUBMED    20221404
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010 . 5(3):e9541."
COMMENT     Sequence Label: pF153 pcDNA3.1(+)SOD1G37R
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(650..667)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    656..767
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      121 cactagtcca gtgtggtgga attccatggc gacgaaggcc gtgtgcgtgc tgaagggcga
      181 cggcccagtg cagggcatca tcaatttcga gcagaaggaa agtaatggac cagtgaaggt
      241 gtggggaagc attaaacgtc tgactgaagg cctgcatgga ttccatgttc atgagtttgg
      301 agataataca gcaggctgta ccagtgcagg tcctcacttt aatcctctat ccagaaaaca
      361 cggtgggcca aaggatgaag agaggcatgt tggagacttg ggcaatgtga ctgctgacaa
      421 agatggtgtg gccgatgtgt ctattgaaga ttctgtgatc tcactctcag gagaccattg
      481 catcattggc cgcacactgg tggtccatga aaaagcagat gacttgggca aaggtggaaa
      541 tgaagaaagt acaaagacag gaaacgctgg aagtcgtttg gcttgtggtg taattgggat
      601 cgcccaataa cgctcgagtc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
      661 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
      721 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
      781 aggtgtcatt ctattctggg ggggtggggt ggggcaggac agcaaggggg
//
