LOCUS       sequence-325978-        8684 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used as a driver for the readout of cPLA2 activation in live cells 
            with an mKate2 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Enyedi B, Jelcic M, Niethammer P
  TITLE     The Cell Nucleus Serves as a Mechanotransducer of Tissue 
            Damage-Induced Inflammation.
  JOURNAL   Cell. 2016 May 19;165(5):1160-1170. doi: 10.1016/j.cell.2016.04.016.
  PUBMED    27203112
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-19- 
            19"; volume: "165"; issue: "5"; pages: "1160-1170"
COMMENT     Sequence Label: pSB-CMV-MCS-Puro dr_cPLA2-mKate2
FEATURES             Location/Qualifiers
     source          1..8684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        71..450
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        451..654
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     605..625
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        699..717
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3034..3744
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRAD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKL"
     CDS             3034..3744
                     /codon_start=1
                     /product="monomeric photoactivatable red fluorescent 
                     protein (Gunewardene et al., 2011)"
                     /label=PAmKate
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKAVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRAD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKL"
     primer_bind     complement(3784..3801)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3790..4014
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        4021..4378
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4021..4212
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4044..4064)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4229..4364
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4406..5005
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4406..4425)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4902..4922
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(5019..5038)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    5048..5129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     5073..5092
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5604..5624)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5604..5622)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5643..5659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5643..5659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5656..5678)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5667..5683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5691..5697,5698..5715,5716..5721))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5736..5757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5874..5891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6045..6633)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6125..6144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6804..7595,7596..7664))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7427..7446
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7665..7769)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7796..8251
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7883..7902)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8093..8114
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8377..8399
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8391..8408
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8392..8408
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8418..8436
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctaagttgac tgtgccttta aacagcttgg aaaattccag aaaatgatgt catggcttta
       61 gaagcttgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      121 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      181 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      241 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      301 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      361 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      421 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      481 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      541 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      601 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
      661 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
      721 acccaagctg gctagcgttt aaacttaagc ttggtaccga gctcggatcc catcgatgcc
      781 acaatgtcca acattatagt tgagcatcag tattctcata gactgaaatt gactgtggtg
      841 agagctgaaa atgtaactaa aggagcattt ggggatttat tggacacccc ggacccttat
      901 gttgagctgt cagtgccaac cacaccagag tcaagaaaac gaacccgcca cattaacaat
      961 gacattaacc ccaagtggaa cgagaccttt gagttcatat tggatcccaa tcaatccaat
     1021 gttttggagg tgacactgat ggatgccaat tatgtcatgg atgaaactct tggcacagca
     1081 aaatattccc tctcaaaact caaagtggca caaatggagc atgtgaccct ctctatcggc
     1141 aagacaacca aagtgttcct cgacttgctc ttggaagttt gcgcatccac agacctgcgc
     1201 ttcagcatga cactgtgtga tcaagagaag ctgttcatgc agacacgcag agacagggtc
     1261 atgctcagca taaagaagct tcttaaaatg gaaaaccctc gtttccttcc atcctcacca
     1321 agagaggttc ccaccatcgc tattctgggc tctggaggag gttttcgtgc aatggtgggc
     1381 ttctctggtg tgatgaaggc tttgtatgaa tctggcgtgt ttgactgtgc aacatatgtg
     1441 gcaggacttt caggatcaac atggtatatg tctatgctgt actctcatcc agagtttcct
     1501 gctaaaggcc caggcgacat taataaagag ctcatgaaca gagtcagtaa taacccactc
     1561 aaactgctcc tgccccaaaa catcaaccgc tacgtcaaag cactgtggaa gaagaaatct
     1621 gctggacagc cggtcacctt cactgacatc ttcgggatgt tgataggaga gacgcttatt
     1681 ccagggagaa tgaacattaa gctgagctca ttgaaaggga aaattaatga aggtcagagc
     1741 cctcttcctc tcttcacctg tctgcacgtc aaacctgacg tctctgagct catgtttgca
     1801 gattgggtgg agttcagccc ttatgaaatt ggcatggcca aatatggcac cttcatgtct
     1861 cctgggctgt ttggcagcaa attcttcatg ggaagtgttg tgaaacaata tgaggaaaac
     1921 cccttacatt tcctgatggg tgtgtgggga agtgcttttt ccatcctctt taaccgggtt
     1981 ttgggtgtga aggagacgac cagcagtagc actatggagg aggagctgga gcaaattaaa
     2041 ccagagcaca ttgtgggtga cgattctgct gataacgagg aggaaactca aagaggaggc
     2101 acagagtctg cagatgcaga ggacgagcgc cagcgtcatg cccaggccag ctgggtacag
     2161 cgcatgttga cctccatcat gggcgacacc actctgttca ccactcgtga gggccgcgca
     2221 ggcaaagtgc acaacttcat gctgggactg aacctgaact caaccctgcc cttctctccc
     2281 ttcagcggca tcacacacca gacctcgctg gaggaggaag tggacgctgt tactgatccg
     2341 gatgagtttg agcgaatcta tgagcctctg gatgtgaaga gcaagaaaat ccatgtggtg
     2401 gacagtggct tgacctttaa cctgccctac cctctcattc tgcggtgtca gagaggagtc
     2461 gacctgatta tttcattcga cttctcagcc cgacccagtg actccagccc accgttcaaa
     2521 gagttactgc tggctgaaaa atgggcccgt atgaataagc tgcccttccc gaagattgat
     2581 tcgaaagtat tcgacagaga gggtctaaaa gagtgctatg tgttcaagcc tgctaaagga
     2641 gacaagaact gccccaccat cattcatttt gtcttggcca acatcaactt ccggaatttt
     2701 aaagcccctg gagttcctcg agattcagac aaggacatag agtttggtga ctttgacata
     2761 tttgacgagc cagcctcccc ttactccact ttcaacttca aatacaacaa ccaggccttc
     2821 aagagacttc atgacctcat ggagttcaat acgcttaaca acatcgaggt tatcaaagaa
     2881 gccattaaag acagcattct gctgagacga gagaacccag cgcgatgttc ggtttccctt
     2941 tcgctcagtg aaatcgaaaa caagaagttc ctgaaacgag acaacagcat tgcaaaacga
     3001 cccaccggag ctggaggaat tcgtcctgga tctatggtgt ctaagggcga agagctgatt
     3061 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
     3121 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcc
     3181 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
     3241 agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct
     3301 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     3361 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     3421 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag
     3481 accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg
     3541 ggcgggggcc acctgatctg caacttgaag accacataca gatccaagaa acccgctaag
     3601 aacctcaaga tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc
     3661 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     3721 agcaaactgg ggcacaaact gtaactcgag cctctagagg gcccgtttaa acccgctgat
     3781 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     3841 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     3901 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     3961 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggaccgga
     4021 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     4081 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     4141 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     4201 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4261 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4321 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     4381 gctagatttt ctgaccctag ccaccatgac cgagtacaag cccacggtgc gcctcgccac
     4441 ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     4501 cacgcgccac accgtcgacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     4561 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     4621 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     4681 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     4741 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtgtcgcc
     4801 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     4861 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     4921 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     4981 catgacccgc aagcccggtg cctgaggatc tcgagctcga tgagtttgga caaaccacaa
     5041 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     5101 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     5161 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     5221 cttaagaggg atcgataagg atctagcttg tggaaggcta ctcgaaatgt ttgacccaag
     5281 ttaaacaatt taaaggcaat gctaccaaat actaattgag tgtatgtaaa cttctgaccc
     5341 actgggaatg tgatgaaaga aataaaagct gaaatgaatc attctctcta ctattattct
     5401 gatatttcac attcttaaaa taaagtggtg atcctaactg acctaagaca gggaattttt
     5461 actaggatta aatgtcagga attgtgaaaa agtgagttta aatgtatttg gctaaggtgt
     5521 atgtaaactt ccgacttcaa ctgtataggg atcctctagc tagagtcgac ctcgaggggg
     5581 ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
     5641 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5701 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5761 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5821 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5881 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5941 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     6001 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     6061 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     6121 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     6181 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaaccgtggc gctttctcat
     6241 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6301 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6361 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6421 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6481 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6541 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6601 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6661 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6721 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6781 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6841 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6901 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6961 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     7021 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     7081 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     7141 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     7201 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     7261 aacttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7321 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7381 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7441 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7501 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7561 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7621 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7681 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7741 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg
     7801 ccctgtagcg gcgcattaag cgcggcggga gtggtggtta cgcgcagcgt gaccgctaca
     7861 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     7921 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     7981 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     8041 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     8101 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     8161 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     8221 aattttaaca aaatattaac gcttacaatt tccattcgcc attcaggctg cgcaactgtt
     8281 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     8341 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     8401 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggagctc ggatccctat
     8461 acagttgaag tcggaagttt acatacactt aagttggagt cattaaaact cgtttttcaa
     8521 ctactccaca aatttcttgt taacaaacaa tagttttggc aagtcagtta ggacatctac
     8581 tttgtgcatg acacaagtca tttttccaac aattgtttac agacagatta tttcacttat
     8641 aattcactgt atcacaattc cagtgggtca gaagtttaca taca
//
