LOCUS       sequence-325979-       11235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for the dual color labeling of cPLA2 and LaminB1 expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11235)
  AUTHORS   Enyedi B, Jelcic M, Niethammer P
  TITLE     The Cell Nucleus Serves as a Mechanotransducer of Tissue 
            Damage-Induced Inflammation.
  JOURNAL   Cell. 2016 May 19;165(5):1160-1170. doi: 10.1016/j.cell.2016.04.016.
  PUBMED    27203112
REFERENCE   2  (bases 1 to 11235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.04"; journalName: "Cell"; date: "2016-05-19- 
            19"; volume: "165"; issue: "5"; pages: "1160-1170"
COMMENT     Sequence Label: pSB-CMV-MCS-Puro cPLA2-mK2-P2A-EGFP-LaminB1
FEATURES             Location/Qualifiers
     source          1..11235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        191..570
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        571..774
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     725..745
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        819..837
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3871..3927
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(3934..3936,3937..3939,3940..4650)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3934..3936,3937..3939,3940..4650)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3979..4000)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4240..4259)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4587..4608
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6455..6472)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6461..6685
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        6692..7049
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6692..6883
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6715..6735)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6900..7035
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7077..7676
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7077..7096)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7573..7593
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(7690..7709)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    7719..7800
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     7744..7763
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8275..8295)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8275..8293)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8314..8330)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8314..8330)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8327..8349)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8338..8354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8362..8368,8369..8386,8387..8392))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8407..8428
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8545..8562)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8716..9304)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8796..8815)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9475..10266,10267..10335))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAAKLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10098..10117
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10336..10440)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      10467..10922
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10554..10573)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10764..10785
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11048..11070
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11062..11079
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11063..11079
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11089..11107
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cagttaggac atctactttg tgcatgacac aagtcatttt tccaacaatt gtttacagac
       61 agattatttc acttataatt cactgtatca caattccagt gggtcagaag tttacataca
      121 ctaagttgac tgtgccttta aacagcttgg aaaattccag aaaatgatgt catggcttta
      181 gaagcttgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      241 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      301 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      361 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      421 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      481 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      541 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      601 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      661 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      721 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
      781 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
      841 acccaagctg gctagcgttt aaacttaagc ttggtaccga gctcggatcc catcgatgcc
      901 acaatgtcca acattatagt tgagcatcag tattctcata gactgaaatt gactgtggtg
      961 agagctgaaa atgtaactaa aggagcattt ggggatttat tggacacccc ggacccttat
     1021 gttgagctgt cagtgccaac cacaccagag tcaagaaaac gaacccgcca cattaacaat
     1081 gacattaacc ccaagtggaa cgagaccttt gagttcatat tggatcccaa tcaatccaat
     1141 gttttggagg tgacactgat ggatgccaat tatgtcatgg atgaaactct tggcacagca
     1201 aaatattccc tctcaaaact caaagtggca caaatggagc atgtgaccct ctctatcggc
     1261 aagacaacca aagtgttcct cgacttgctc ttggaagttt gcgcatccac agacctgcgc
     1321 ttcagcatga cactgtgtga tcaagagaag ctgttcatgc agacacgcag agacagggtc
     1381 atgctcagca taaagaagct tcttaaaatg gaaaaccctc gtttccttcc atcctcacca
     1441 agagaggttc ccaccatcgc tattctgggc tctggaggag gttttcgtgc aatggtgggc
     1501 ttctctggtg tgatgaaggc tttgtatgaa tctggcgtgt ttgactgtgc aacatatgtg
     1561 gcaggacttt caggatcaac atggtatatg tctatgctgt actctcatcc agagtttcct
     1621 gctaaaggcc caggcgacat taataaagag ctcatgaaca gagtcagtaa taacccactc
     1681 aaactgctcc tgccccaaaa catcaaccgc tacgtcaaag cactgtggaa gaagaaatct
     1741 gctggacagc cggtcacctt cactgacatc ttcgggatgt tgataggaga gacgcttatt
     1801 ccagggagaa tgaacattaa gctgagctca ttgaaaggga aaattaatga aggtcagagc
     1861 cctcttcctc tcttcacctg tctgcacgtc aaacctgacg tctctgagct catgtttgca
     1921 gattgggtgg agttcagccc ttatgaaatt ggcatggcca aatatggcac cttcatgtct
     1981 cctgggctgt ttggcagcaa attcttcatg ggaagtgttg tgaaacaata tgaggaaaac
     2041 cccttacatt tcctgatggg tgtgtgggga agtgcttttt ccatcctctt taaccgggtt
     2101 ttgggtgtga aggagacgac cagcagtagc actatggagg aggagctgga gcaaattaaa
     2161 ccagagcaca ttgtgggtga cgattctgct gataacgagg aggaaactca aagaggaggc
     2221 acagagtctg cagatgcaga ggacgagcgc cagcgtcatg cccaggccag ctgggtacag
     2281 cgcatgttga cctccatcat gggcgacacc actctgttca ccactcgtga gggccgcgca
     2341 ggcaaagtgc acaacttcat gctgggactg aacctgaact caaccctgcc cttctctccc
     2401 ttcagcggca tcacacacca gacctcgctg gaggaggaag tggacgctgt tactgatccg
     2461 gatgagtttg agcgaatcta tgagcctctg gatgtgaaga gcaagaaaat ccatgtggtg
     2521 gacagtggct tgacctttaa cctgccctac cctctcattc tgcggtgtca gagaggagtc
     2581 gacctgatta tttcattcga cttctcagcc cgacccagtg actccagccc accgttcaaa
     2641 gagttactgc tggctgaaaa atgggcccgt atgaataagc tgcccttccc gaagattgat
     2701 tcgaaagtat tcgacagaga gggtctaaaa gagtgctatg tgttcaagcc tgctaaagga
     2761 gacaagaact gccccaccat cattcatttt gtcttggcca acatcaactt ccggaatttt
     2821 aaagcccctg gagttcctcg agattcagac aaggacatag agtttggtga ctttgacata
     2881 tttgacgagc cagcctcccc ttactccact ttcaacttca aatacaacaa ccaggccttc
     2941 aagagacttc atgacctcat ggagttcaat acgcttaaca acatcgaggt tatcaaagaa
     3001 gccattaaag acagcattct gctgagacga gagaacccag cgcgatgttc ggtttccctt
     3061 tcgctcagtg aaatcgaaaa caagaagttc ctgaaacgag acaacagcat tgcaaaacga
     3121 cccaccggag ctggaggaat tcgtcctgga tctatggtgt ctaagggcga agagctgatt
     3181 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag
     3241 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggcc
     3301 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc
     3361 agcaaaacct tcatcaacca cacccagggc atccccgact tctttaagca gtccttccct
     3421 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
     3481 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
     3541 ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc ctccaccgag
     3601 accctgtacc ccgctgacgg cggcctggaa ggcagagccg acatggccct gaagctcgtg
     3661 ggcgggggcc acctgatctg caacttgaag accacataca gatccaagaa acccgctaag
     3721 aacctcaaga tgcccggcgt ctactatgtg gacagaagac tggaaagaat caaggaggcc
     3781 gacaaagaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct
     3841 agcaaactgg ggcacaaact gggaagcgga gctactaact tcagcctgct gaagcaggct
     3901 ggagacgtgg aggagaaccc tggacctgga tccatggtga gcaagggcga ggagctgttc
     3961 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     4021 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     4081 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     4141 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     4201 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     4261 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     4321 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     4381 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     4441 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     4501 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     4561 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     4621 atcactctcg gcatggacga gctgtacaag agatctatgg cgagcgcggc gtgtactccg
     4681 gccgggcagc agtcgttgtc ccgcggcacc agcagcactc cactgagccc cgcgcggatc
     4741 tccaggctgc aagagaaaga ggagctgcag cagctcaatg accggcttgc cgcctacatc
     4801 gacacagtcc gtagtctgga gtccgagaac agcgtcctgc aaatacagat cagagaacga
     4861 gaggatgtgc ggaaccgcga gctcacgggg ctcagaacac tctacgaaac cgagctggcg
     4921 gacgcacgga aggcgcttga tgacacggca cgggagcgcg cacgattgca gattgaactg
     4981 ggcaagatta aaagcgagca agagcagcta cagcacacat atgccaagaa agatgctgac
     5041 ctcaccagct ctcagaaccg actaaaagat gttgaagccc tgttgaacgc taaagaagct
     5101 gcactaaaca cagccgtctc tgaaaggaag actctggaaa acactctatc tgatctgcag
     5161 atccgtgtcc aggagttgga aggagggttg atctccgcta agaagcagct gtcagatgag
     5221 accctgctgc gtgtggatct ggagaaccgc tgtcagagcc ttatggagga acttgaattt
     5281 aggaaaaaca tgtttgaaga ggaaatcaaa gatacacgtc gtagacatga gacccgtctt
     5341 gtcgaggtgg attcaggtcg tcagatggag tatgagttta aactggctca ggctctgact
     5401 gacatgagac agcagcatga tgagcaagtc aaactttata aggaggagat ggagcagaca
     5461 tacgtgtcca agctggagaa cattcgtttg tcatctgaga tgaacagctc ttcagctagt
     5521 atggcccgtg aggagcttcg agagtctaca ctgagagttg agagtttggc aggtcaactt
     5581 gcaaatctgc agaaagaggc tcgtgcctgg caggatcgca ttaatgaact ggagggggct
     5641 cttagccatg agaaagattt aagccgtagg ttgctggcag agaaagagcg cgagattgct
     5701 gagatcagag ccaagatgca gcagcagctg gatgagtatg agcaactgct ggacgtcaaa
     5761 ctagcgctgg acatggagat caatgcttac agaaagcttc tggagggaga agaagaaagg
     5821 ttgaaactgt ctccaagtcc ttcatctcgt gtgactgtgt cacgagcatc ctccagtcgc
     5881 agtgttcgta ctacccgtgg caagagaaag cgtgtggatg tggaggagtc ggaggcaagc
     5941 agcagtgtca gtatcgctca ttctgcatct gccactggaa acgtctccat cgatgagctg
     6001 gatgtagatg ggaagttcat ccgcttgcac aacaactctg agcaggacca gccgatggca
     6061 ggatttcagc tgaccaggac tattggagaa gttactgcaa catataaatt cactgcaaaa
     6121 tacaatctga aggctggaca gaaagtgacg atctgggcat ctaatgcagg cataagctcc
     6181 aaccctcctg ctgacctgat atggaagaac cagccttcat gggggactgg ggagaacata
     6241 aaagtggctc ttttgagccc agcaggagag gaagtagctg tgagaagcac agtattcaag
     6301 actgcagtgg aagatgaaga cgacgatgag gaggaagaaa aggaagctga agtcattgag
     6361 gagcatttct atcaccagca gggagatcca cgcgctgcta agagaggatg tttgatcatg
     6421 taatctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     6481 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6541 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6601 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6661 gctggggatg cggtgggctc tatggaccgg actgaggcgg aaagaaccag ctgtggaatg
     6721 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6781 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6841 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6901 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6961 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7021 gcttttttgg aggcctaggc ttttgcaaaa agctagattt tctgacccta gccaccatga
     7081 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     7141 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     7201 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     7261 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     7321 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     7381 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     7441 cgtggttcct ggccaccgtc ggcgtgtcgc ccgaccacca gggcaagggt ctgggcagcg
     7501 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     7561 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     7621 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaggat
     7681 ctcgagctcg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     7741 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     7801 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     7861 taaagcaagt aaaacctcta caaatgtggt acttaagagg gatcgataag gatctagctt
     7921 gtggaaggct actcgaaatg tttgacccaa gttaaacaat ttaaaggcaa tgctaccaaa
     7981 tactaattga gtgtatgtaa acttctgacc cactgggaat gtgatgaaag aaataaaagc
     8041 tgaaatgaat cattctctct actattattc tgatatttca cattcttaaa ataaagtggt
     8101 gatcctaact gacctaagac agggaatttt tactaggatt aaatgtcagg aattgtgaaa
     8161 aagtgagttt aaatgtattt ggctaaggtg tatgtaaact tccgacttca actgtatagg
     8221 gatcctctag ctagagtcga cctcgagggg gggcccggta cccagctttt gttcccttta
     8281 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8341 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8401 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8461 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     8521 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     8581 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     8641 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     8701 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     8761 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     8821 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     8881 tctcccttcg ggaaccgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     8941 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9001 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9061 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9121 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9181 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9241 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9301 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9361 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9421 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9481 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     9541 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     9601 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     9661 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     9721 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     9781 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     9841 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     9901 ctccttcggt cctccgatcg ttgtcagaag taacttggcc gcagtgttat cactcatggt
     9961 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10021 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10081 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10141 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10201 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10261 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10321 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10381 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10441 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
    10501 agtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    10561 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    10621 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10681 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10741 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10801 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    10861 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    10921 ttccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
    10981 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
    11041 gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta atacgactca
    11101 ctatagggcg aattggagct cggatcccta tacagttgaa gtcggaagtt tacatacact
    11161 taagttggag tcattaaaac tcgtttttca actactccac aaatttcttg ttaacaaaca
    11221 atagttttgg caagt
//
