LOCUS       sequence-326227-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-fragment of the wtBlc‐split‐Zip protein. This plasmid needs to be 
            co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain 
            wtBlc‐split-Zip protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Bozhanova NG, Calcutt MW, Beavers WN, Brown BP, Skaar EP, Meiler J
  TITLE     Lipocalin Blc is a potential heme-binding protein.
  JOURNAL   FEBS Lett. 2021 Jan;595(2):206-219. doi: 10.1002/1873-3468.14001. 
            Epub 2020 Dec 3.
  PUBMED    33210733
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/1873-3468.14001"; journalName: "FEBS Lett"; date: 
            "2021-01"; volume: "595"; issue: "2"; pages: "206-219"
COMMENT     Sequence Label: pET11a-Z-N-wtBlc
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             430..441
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(519..537)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      533..580
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(755..760,761..777,778..783))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(810..828)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgggtc atcaccacca ccatcacctc
       61 gaatcaacca gcttgtacaa gaaatccagc tccacgccgc cgcgtggcgt gaccgtagta
      121 aataatttcg acgccaaacg ttatctcggt acctggtatg agattgcccg ttttgatcac
      181 cgctttgaac gtggactgga aaaagtcacc gcaacataca gcctgcgtga tgacggcggc
      241 ctgaatgtca ttaataaagg ctataaccct gacagaggaa tgtggcagca gagtgaaggg
      301 aaagcgtact ttaccggcgc accaactcgc gctgcgctga aagtgtcatt ctttggtggc
      361 tctggctctg gctcgagcgc cctcaaaaaa gaattgcagg caaacaaaaa agaacttgcg
      421 cagctgaagt gggagttaca agctctgaaa aaggaactgg cgcagtaagg atccggctgc
      481 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      541 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc
      601 cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc
      661 agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga
      721 ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt
      781 caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      841 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
//
