LOCUS       sequence-326364-       10009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral CRE expression tracked by mScarlet-I fluorescence 
            marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10009)
  AUTHORS   Chan W, Gottschalk RA, Yao Y, Pomerantz JL, Germain RN
  TITLE     Efficient Immune Cell Genome Engineering with Enhanced CRISPR 
            Editing Tools.
  JOURNAL   Immunohorizons. 2021 Feb 23;5(2):117-132. doi: 
            10.4049/immunohorizons.2000082.
  PUBMED    33622708
REFERENCE   2  (bases 1 to 10009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/immunohorizons"; journalName: "Immunohorizons"; date: 
            "2021-02-23- 23"; volume: "5"; issue: "2"; pages: "117-132"
COMMENT     Sequence Label: LES2A-CRE
FEATURES             Location/Qualifiers
     source          1..10009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2650..2861
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2880..2889
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2886..3581
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(3019..3037)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3287..3306
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             3591..3647
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3648..4676
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="SNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     SVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     RPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     GIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     WISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     YLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     GD"
     misc_feature    4701..5289
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4754..4774)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5172..5183)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5292..5308)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5293..5309)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5814..5994
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6020..6037)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6026..6250
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6296..6724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6383..6402)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6593..6614
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6733..6753)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6738..7067
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6918..7053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6980..6999
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7115..7162
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7181..7555
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7685..7806
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7722..7741)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7776..7795
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7855..7871)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7855..7871)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7868..7890)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7879..7895
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7903..7909,7910..7927,7928..7933))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7948..7969
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8086..8103)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8257..8845)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8337..8356)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9016..9807,9808..9876))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9639..9658
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9877..9981)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg
     2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
     2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
     2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
     2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagagcgctg
     2881 ccaccatggt gagcaagggc gaggcagtga tcaaggagtt catgcggttc aaggtgcaca
     2941 tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     3001 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttctcct
     3061 gggacatcct gtcccctcag ttcatgtacg gctccagggc cttcatcaag caccccgccg
     3121 acatccccga ctactataag cagtccttcc ccgagggctt caagtgggag cgcgtgatga
     3181 acttcgagga cggcggcgcc gtgaccgtga cccaggacac ctccctggag gacggcaccc
     3241 tgatctacaa ggtgaagctc cgcggcacca acttccctcc tgacggcccc gtaatgcaga
     3301 agaagacaat gggctgggaa gcatccaccg agcggttgta ccccgaggac ggcgtgctga
     3361 agggcgacat taagatggcc ctgcgcctga aggacggcgg ccgctacctg gcggacttca
     3421 agaccaccta caaggccaag aagcccgtgc agatgcccgg cgcctacaac gtcgaccgca
     3481 agttggacat cacctcccac aacgaggact acaccgtggt ggaacagtac gaacgctccg
     3541 agggccgcca ctccaccggc ggcatggacg agctgtacaa gggatccggc gcaacaaact
     3601 tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgtcc aatttactga
     3661 ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga
     3721 acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc
     3781 tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg
     3841 cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt ctggcagtaa
     3901 aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtcc gggctgccac
     3961 gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg
     4021 atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc
     4081 gttcactcat ggaaaatagc gatcgctgcc aggatatacg taatctggca tttctgggga
     4141 ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt aaagatatct
     4201 cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg ctggttagca
     4261 ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag cgatggattt
     4321 ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc agaaaaaatg
     4381 gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg
     4441 aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct
     4501 ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa
     4561 taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc
     4621 gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gattaagaat
     4681 tcgatatcaa gcttatcggt aatcaacctc tggattacaa aatttgtgaa agattgactg
     4741 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4801 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     4861 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     4921 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     4981 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     5041 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     5101 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     5161 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     5221 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     5281 cctccccgca tcgataccgt cgacctcgag acctagaaaa acatggagca atcacaagta
     5341 gcaatacagc agctaccaat gctgattgtg cctggctaga agcacaagag gaggaggagg
     5401 tgggttttcc agtcacacct caggtacctt taagaccaat gacttacaag gcagctgtag
     5461 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     5521 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga
     5581 actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag
     5641 taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac
     5701 accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg
     5761 acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc
     5821 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5881 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5941 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
     6001 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
     6061 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
     6121 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
     6181 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
     6241 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
     6301 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     6361 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     6421 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     6481 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     6541 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     6601 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     6661 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     6721 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     6781 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6841 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6901 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6961 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     7021 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     7081 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
     7141 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
     7201 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
     7261 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
     7321 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
     7381 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
     7441 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
     7501 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
     7561 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     7621 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     7681 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     7741 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     7801 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7861 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7921 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7981 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     8041 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     8101 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     8161 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     8221 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     8281 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     8341 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     8401 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     8461 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     8521 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8581 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8641 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8701 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8761 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8821 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8881 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8941 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     9001 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     9061 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     9121 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     9181 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     9241 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     9301 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9361 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9421 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9481 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9541 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9601 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9661 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9721 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9781 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9841 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9901 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9961 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
