LOCUS       sequence-326369-        8963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA vector for targeting human H11 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Pryzhkova MV, Jordan PW
  TITLE     Adaptation of Human Testicular Niche Cells for Pluripotent Stem Cell
            and Testis Development Research.
  JOURNAL   Tissue Eng Regen Med. 2020 Apr;17(2):223-235. doi: 
            10.1007/s13770-020-00240-0. Epub 2020 Feb 29.
  PUBMED    32114677
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s13770-020-00240-0"; journalName: "Tissue Eng Regen Med"; 
            date: "2020-04"; volume: "17"; issue: "2"; pages: "223-235"
COMMENT     Sequence Label: p276 eSpCas9_2gRNAs_hH11
FEATURES             Location/Qualifiers
     source          1..8963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        270..345
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        436..684
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        705..780
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        870..1155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     intron          1463..1690
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     regulatory      1702..1711
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1711..1776
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1783..1803
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1828..4365,4366..4368,4369..4830,4831..4833,4834..5001,
                     5002..5004,5005..5928)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system, mutated to improve targeting 
                     specificity (Slaymaker et al., 2016)"
                     /label=eSpCas9(1.1)
                     /note="carries the mutations K848A, K1003A, and R1060A"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLADDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPALESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKAPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5929..5976
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(6004..6021)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6010..6217
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   6226..6366
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6226..6355
                     /label=AAV2 ITR
     rep_origin      6441..6896
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6528..6547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6738..6759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6913..6932)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7032..7054
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7092..7110)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7178..7282
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7283..7351,7352..8143)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEALPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7501..7520)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8314..8902
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8803..8822
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg ccaacaggtc agtttatacg ttttagagct agaaatagca agttaaaata
      301 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag
      361 ctagaaatag caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag
      421 acaaatggct ctagagaggg cctatttccc atgattcctt catatttgca tatacgatac
      481 aaggctgtta gagagataat tggaattaat ttgactgtaa acacaaagat attagtacaa
      541 aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt
      601 aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat
      661 atcttgtgga aaggacgaaa caccgattcc tgcaagtaca cggcgtttta gagctagaaa
      721 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
      781 ttttttgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt tttagcgcgt
      841 gcgccaattc tgcagacaaa tggggtaccc gttacataac ttacggtaaa tggcccgcct
      901 ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc
      961 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     1021 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     1081 tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     1141 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     1201 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     1261 gcgggggggg gggggggggc gssccmcggg gggggggggg ggggggsgsg sgssaggcgg
     1321 ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca
     1381 gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa
     1441 aaagcgaagc gcgcggcggg cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc
     1501 cgccgccgcc tcgcgccgcc cgccccggct ctgactgacc gcgttactcc cacaggtgag
     1561 cgggcgggac ggcccttctc ctccgggctg taattagctg agcaagaggt aagggtttaa
     1621 gggatggttg gttggtgggg tattaatgtt taattacctg gagcacctgc ctgaaatcac
     1681 tttttttcag gttggaccgg tgccaccatg gactataagg accacgacgg agactacaag
     1741 gatcatgata ttgattacaa agacgatgac gataagatgg ccccaaagaa gaagcggaag
     1801 gtcggtatcc acggagtccc agcagccgac aagaagtaca gcatcggcct ggacatcggc
     1861 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
     1921 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
     1981 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
     2041 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
     2101 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
     2161 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
     2221 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc cgacctgcgg
     2281 ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct gatcgagggc
     2341 gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt gcagacctac
     2401 aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa ggccatcctg
     2461 tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct gcccggcgag
     2521 aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac ccccaacttc
     2581 aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga cacctacgac
     2641 gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct gtttctggcc
     2701 gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa caccgagatc
     2761 accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca ccaggacctg
     2821 accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga gattttcttc
     2881 gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca ggaagagttc
     2941 tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact gctcgtgaag
     3001 ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag catcccccac
     3061 cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt ttacccattc
     3121 ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc ctactacgtg
     3181 ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag cgaggaaacc
     3241 atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca gagcttcatc
     3301 gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc caagcacagc
     3361 ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata cgtgaccgag
     3421 ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt ggacctgctg
     3481 ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt caagaaaatc
     3541 gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc ctccctgggc
     3601 acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa tgaggaaaac
     3661 gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag agagatgatc
     3721 gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag
     3781 cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg catccgggac
     3841 aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc caacagaaac
     3901 ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca gaaagcccag
     3961 gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg cagccccgcc
     4021 attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa agtgatgggc
     4081 cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac cacccagaag
     4141 ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa agagctgggc
     4201 agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga gaagctgtac
     4261 ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga catcaaccgg
     4321 ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctggccga cgactccatc
     4381 gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa cgtgccctcc
     4441 gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc caagctgatt
     4501 acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag cgaactggat
     4561 aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa gcacgtggca
     4621 cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct gatccgggaa
     4681 gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga tttccagttt
     4741 tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct gaacgccgtc
     4801 gtgggaaccg ccctgatcaa aaagtaccct gcgctggaaa gcgagttcgt gtacggcgac
     4861 tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat cggcaaggct
     4921 accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg
     4981 gccaacggcg agatccggaa ggcgcctctg atcgagacaa acggcgaaac cggggagatc
     5041 gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg
     5101 aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc
     5161 aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc
     5221 ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc
     5281 aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc
     5341 agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag
     5401 gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga
     5461 atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat
     5521 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat
     5581 gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag
     5641 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
     5701 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
     5761 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac
     5821 cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
     5881 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacaa aaggccggcg
     5941 gccacgaaaa aggccggcca ggcaaaaaag aaaaagtaag aattcctaga gctcgctgat
     6001 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6061 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6121 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6181 gggaggattg ggaagagaat agcaggcatg ctggggagcg gccgcaggaa cccctagtga
     6241 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
     6301 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
     6361 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     6421 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6481 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
     6541 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6601 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
     6661 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6721 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
     6781 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
     6841 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
     6901 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     6961 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     7021 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     7081 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     7141 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7201 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7261 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7321 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7381 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7441 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7501 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7561 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7621 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7681 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7741 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccctaccaaa
     7801 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7861 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7921 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7981 tggagccggt gagcgtggaa gccgcggtat cattgcagca ctggggccag atggtaagcc
     8041 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     8101 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     8161 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8221 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8281 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8341 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8401 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8461 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8521 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8581 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8641 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8701 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8761 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8821 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8881 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8941 ttgctggcct tttgctcaca tgt
//
