LOCUS       sequence-326399-        9377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  constitutive TIR1 expression vector for mouse H11 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Pryzhkova MV, Xu MJ, Jordan PW
  TITLE     Adaptation of the AID system for stem cell and transgenic mouse 
            research.
  JOURNAL   Stem Cell Res. 2020 Nov 5;49:102078. doi: 10.1016/j.scr.2020.102078.
  PUBMED    33202307
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Res."; date: "2020-11-5"; volume: "49:102078. doi"; pages: " 
            10.1016/j.scr.2020.102078"
COMMENT     Sequence Label: p285 TIR1_HA_mH11
FEATURES             Location/Qualifiers
     source          1..9377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(35..41,42..59,60..65))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    80..101
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(218..235)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(389..977)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(469..488)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1148..1939,1940..2008))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1771..1790
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2009..2113)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2140..2595
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2227..2246)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2437..2458
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2721..2743
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2735..2752
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2736..2752
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3557..4735
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3788..4726
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4683..4703
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4755..6479
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             6489..6515
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    6635..6756
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6672..6691)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6726..6745
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(6796..6829)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6866..7365
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6901..6924)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7298..7317
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7405..8004
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7405..7424)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7901..7921
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(8585..8618)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
       61 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
      661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
      721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
      781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
      841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
      901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
      961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     1681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     1741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     1801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     1861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     1921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     1981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     2161 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2221 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2281 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2341 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2401 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2461 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2521 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2581 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     2641 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     2701 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct
     2761 gatctataca ttgaatcaat attggcaatt agccatatta gtcattggtt atatagcata
     2821 aatcaatatt ggctattggc cattgcatac gtttaggact tctggaattt cttctgcaca
     2881 taaagttctg atagcattag tttaagctgg actaaccctg aaagtagctt gtggcaagta
     2941 tcaaggaatc aatattatac tctacaaaat caaagtttac agagaagtca tatagtaatt
     3001 tttctgaaat ttactggcac aatgttaatc cagcctgact ccaactaatt aatggtcaca
     3061 ttaatttaag tctttccctt gcctctgctg cattagtttc tctcaaaatt gttaacttac
     3121 aacttgaagt ctggtattat aaattgaatg taaagcattc tgaaagatac tatactgatt
     3181 gcaggttttt cagtcaggtt caagctaatt tgaccagtca ttggattaat tatggatctg
     3241 gggccataaa tgctatttta attccactat agagattaaa ataagccatt ctccatttca
     3301 taatattcta ttggactttg actgcagggg cctccaagtc ttgacagtag attataatcc
     3361 ttcagctgcc cactctactg gaggaggaca aactggtcac ttttcagcaa aacctggctg
     3421 tggatcaggg cagtctggta cttccaagct cattagatgc catcatgctc tcactgcctc
     3481 ctcagcttca agaggaatct ggaaaaagca gtcccactgg tcaggaaagg aacactagtg
     3541 cacaaacggc tcgaggggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
     3601 ccccgagaag ttgtggggag gggtcggcaa ttgaagcggt gcctagagaa ggtggcgcgg
     3661 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
     3721 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
     3781 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
     3841 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg
     3901 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc
     3961 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg
     4021 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc
     4081 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat
     4141 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc
     4201 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg
     4261 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct
     4321 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg
     4381 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag
     4441 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc
     4501 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga
     4561 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc
     4621 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat
     4681 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgagggga
     4741 tcgtcgacgc caccatgacc tacttccccg aggaagtggt ggaacacatc ttcagctttc
     4801 tgcccgccca gagggacaga aacaccgtgt ccctcgtgtg caaagtgtgg tacgagatcg
     4861 agagactgag cagacggggc gtgttcgtgg gcaactgtta cgctgtgcgg gctggcagag
     4921 tggccgccag atttcctaat gtgcgggccc tgaccgtgaa gggcaagcct cacttcgccg
     4981 acttcaacct ggtgccccct gattggggcg gctacgctgg accttggatc gaggctgctg
     5041 ccagaggctg tcacggcctg gaagaactga gaatgaagag aatggtggtg tccgacgaga
     5101 gcctggaact gctggccaga agcttcccca gattcagagc cctggtgctg atctcctgcg
     5161 agggcttctc tacagatggc ctggccgccg tggccagcca ttgcaagctg ctgagagagc
     5221 tggatctcca ggaaaacgag gtggaagata gaggccccag atggctgagc tgcttccccg
     5281 acagctgtac cagcctggtg tccctgaact tcgcctgcat caagggggaa gtgaacgccg
     5341 gcagcctgga aagactggtg tccagaagcc ccaacctgag aagcctgaga ctgaacagat
     5401 ccgtgtccgt ggacaccctg gccaagatcc tgctgagaac ccctaacctg gaagatctgg
     5461 gcaccggcaa cctgaccgac gacttccaga ccgagagcta cttcaagctg accagcgccc
     5521 tggaaaagtg caagatgctg cggagcctga gcggcttctg ggacgcctct cctgtgtgcc
     5581 tgagcttcat ctaccccctg tgcgcccagc tgaccggcct gaacctgtct tacgccccta
     5641 cactggacgc cagcgacctg accaagatga tcagcagatg cgtgaagctc cagagactgt
     5701 gggtgctgga ctgcatcagc gacaagggac tccaggtggt ggccagctcc tgcaaggatc
     5761 tccaggaact gagagtgttc cccagcgact tctacgtggc cggctacagc gccgtgaccg
     5821 aagagggact ggtggctgtg tctctgggct gccccaagct gaacagcctg ctgtacttct
     5881 gccaccagat gaccaacgcc gccctcgtga ccgtggccaa gaactgcccc aacttcacca
     5941 ggttcagact gtgcatcctg gaacccggca agcccgacgt tgtgaccagc cagccactgg
     6001 atgagggctt cggagccatc gtgcgcgagt gcaagggact ccagagactg agcatcagcg
     6061 gcctgctgac cgacaaggtg ttcatgtaca tcgggaagta cgccaagcag ctggaaatgc
     6121 tgtctatcgc cttcgctggc gactccgaca agggcatgat gcacgtgatg aacggctgca
     6181 agaacctgcg gaaactggaa atcagagaca gccccttcgg cgacgctgcc ctgctgggca
     6241 acttcgccag atacgagaca atgagatccc tgtggatgag cagctgcaac gtgacactga
     6301 agggctgcca ggtgctggcc agcaagatgc ccatgctgaa cgtggaagtg atcaacgaga
     6361 gggacggcag caatgagatg gaagagaacc acggcgacct gcctaaggtg gaaaagctgt
     6421 acgtgtacag aaccacagcc ggcgctaggg acgacgcccc taacttcgtg aagatcctgg
     6481 gcggatctta cccatacgac gtaccagatt acgcttaggc gatcgcctga tcataatcag
     6541 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     6601 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     6661 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     6721 tagttgtggt ttgtccaaac tcatcaatgt atcttaacgc gtcgccagta ctagtgaacc
     6781 tcttcgaggg acctaataac ttcgtatagc atacattata cgaagttata ttaagggttc
     6841 cggatctcga cctcgaaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     6901 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     6961 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     7021 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     7081 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     7141 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     7201 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     7261 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     7321 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg catccatcta
     7381 gatctcgagc agctgaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc
     7441 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     7501 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     7561 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     7621 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     7681 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     7741 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     7801 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     7861 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     7921 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     7981 atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg
     8041 agcgcacgac cccatgcatc gatgatatca gatccccggg atgcagaaat tgatgatcta
     8101 ttaaacaata aagatgtcca ctaaaatgga agtttttcct gtcatacttt gttaagaagg
     8161 gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg tgggggtggg
     8221 gtgggattag ataaatgcct gctctttact gaaggctctt tactattgct ttatgataat
     8281 gtttcatagt tggatatcat aatttaaaca agcaaaacca aattaagggc cagctcattc
     8341 ctcccactca tgatctatag atctatagat ctctcgtggg atcattgttt ttctcttgat
     8401 tcccactttg tggttctaag tactgtggtt tccaaatgtg tcagtttcat agcctgaaga
     8461 acgagatcag cagcctctgt tccacataca cttcattctc agtattgttt tgccaagttc
     8521 taattccatc agaagctggt cgagatccta agcttggctg gacgtaaact cctcttcaga
     8581 cctaataact tcgtatagca tacattatac gaagttatat taagggttat tgaatatgat
     8641 cggaattgcg gccccatatg gtttctgtga aaatgccttt tcaatcatta acctcttaaa
     8701 tgagtgttag catcctaact aatgagtggt gcagaatagt gggtctgctt agcttaacta
     8761 aggccaagaa aacaaaacag gaaattcatt ccatgtcatg agactcatac tacgaggttc
     8821 ccttagacct caggagaaaa agtctttggc tgtaagaaca cacctcagtg gatgtggtag
     8881 actatgcctt tactcttttt tttttttttt ttttttttgg gttttttcga gacagggttt
     8941 ctctgtgtag ccttggctgt cctggaactc actttgttga ccaggctggc ctcgaactca
     9001 gagatccgcc tgcctctgcc tctcaagtgc tagaattaaa agtgtgcgcc accactccca
     9061 gctatataga ctatgccttt aatcaggaca cttgggaggc agggggatct caaattcaat
     9121 tccagacaga tgactgacaa acacacacat acaccattcg tcttttcttt ttttcttttt
     9181 tcttttgaaa acaggtttct ctatgcagcc ctggctttcc tagaattcaa tctatagacc
     9241 cagctggcct caaacacaga acctcttgcc tctgcctcct caacactatg actaaaggtg
     9301 tgaaccacca ccaccaccac ccacctggcc aaagaatgaa ttgagtgaat aagtacgagt
     9361 gcaactctcc agggccg
//
