LOCUS       sequence-326503-         345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pGBKT7 yeast two-hybrid plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Bentham AR, Petit-Houdenot Y, Win J, Chuma I, Terauchi R, Banfield 
            MJ, Kamoun S, Langner T
  TITLE     A single amino acid polymorphism in a conserved effector of the 
            multihost blast fungus pathogen expands host-target binding 
            spectrum.
  JOURNAL   PLoS Pathog. 2021 Nov 10;17(11):e1009957. doi: 
            10.1371/journal.ppat.1009957. eCollection 2021 Nov.
  PUBMED    34758051
REFERENCE   2  (bases 1 to 345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2021-11-10"; volume: "17(11):e1009957. doi"; pages:
            " 10.1371/journal.ppat.1009957. eCollection 2021 Nov"
COMMENT     Sequence Label: pTL342
FEATURES             Location/Qualifiers
     source          1..345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(248..265)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(311..334)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gaattcatga gcaccattgt gatgatggtg gacctggaat gcgatcgctg ctatcgcaaa
       61 attcgccgcg tgctgtgcaa actgcaggac aaagccagca tcaaagccat cagctatgac
      121 gagaaaaata ataccgtgac cgtggccggt ccgtttgacg cagatgaagt gagcgaccgt
      181 ctgtgtagca gcgccggcaa agtgattacc gatattcgcg tggtgggtgg tgccaaaccg
      241 atgccgggtg gtggtggtgg tggtgcaaaa gcccatgcaa acaaaccggc cggcaaagat
      301 ggtagcgcag gtggtggtgg tggtggtggt ggtggtaaag gatcc
//
