LOCUS       sequence-326561-        2337 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0411
FEATURES             Location/Qualifiers
     source          1..2337
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(41..700)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     615..634
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     862..880
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(884..927)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      1240..1297
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1321..1338)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1492..2080)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1572..1591)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(join(2263..2337,1..20))
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
       61 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
      121 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
      181 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
      241 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
      301 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
      361 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
      421 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
      481 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
      541 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
      601 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
      661 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
      721 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
      781 tggaacctct tacgtgcccg atcaactcga gtgccacctg acgtctaaga aaccattatt
      841 atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag ataaaaaaaa
      901 tccttagctt tcgctaagga tgatttctgg aattcggtct caggagggag gcgtgtcgtt
      961 ttagtgagga accacgtcta caaggtaggt cacgaccact atgccatgag ctaagcacat
     1021 acgtcagtat cagcttagca aaaccattat tgcgtctgca cctcacatgt aggctgaaga
     1081 taagataata atgttgaaga taagaacgac cactatgccc aattagctga cgtaagggat
     1141 gacgcacagg ttgtctgcac ctcacatgta ctatataagg ttttgctatt cattgaaagc
     1201 agtagtgact gatttgtata tatactcgag accctgcagt ccggcaaaaa agggcaaggt
     1261 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc
     1321 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1381 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1441 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag
     1501 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1561 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1621 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1681 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1741 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1801 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1861 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     1921 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     1981 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2041 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2101 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2161 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2221 aagtatatat gagtaaactt ggtctgacag ctcgaggctt ggattctcac caataaaaaa
     2281 cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg tcattac
//
