LOCUS       sequence-326562-        2291 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPYC0CM0398
FEATURES             Location/Qualifiers
     source          1..2291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     184..203
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     431..449
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(453..496)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     terminator      763..820
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(844..861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1015..1603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1095..1114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1786..1880)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(1901..2291,1..269))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
       61 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
      121 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
      181 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
      241 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
      301 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
      361 acgtgcccga tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt
      421 aacctataaa aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt
      481 cgctaaggat gatttctgga attcggtctc aggagggagg cgtgtcgttt tagtgaggta
      541 tgcccaattg aagataagat aataatgttg aagataagag tgggagccac catgcccaat
      601 taaagacctc tagctttgtc aaaagctaaa aaagatgatg ctcacgacca caattaggtt
      661 gatgacgtaa gccatgacgt ctacatgtag gctctatata aggttttgct attcattgaa
      721 agcagtagtg actgatttgt atatatactc gagaccctgc agtccggcaa aaaagggcaa
      781 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc
      841 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      901 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      961 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1021 caggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1081 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1141 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1201 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1261 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1321 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1381 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1441 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1501 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1561 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1621 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1681 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1741 ctaaagtata tatgagtaaa cttggtctga cagctcgagg cttggattct caccaataaa
     1801 aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg aggtcattac
     1861 tggatctatc aacaggagtc caagcgagct cgatatcaaa ttacgccccg ccctgccact
     1921 catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg
     1981 catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc
     2041 ccatggtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg
     2101 tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga
     2161 aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc
     2221 ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa
     2281 cggtgtaaca a
//
