LOCUS       sequence-326564-        2175 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Phytobricks Level 0 part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Cai YM, Kallam K, Tidd H, Gendarini G, Salzman A, Patron NJ
  TITLE     Rational design of minimal synthetic promoters for plants.
  JOURNAL   Nucleic Acids Res. 2020 Aug 28. pii: 5897334. doi: 
            10.1093/nar/gkaa682.
  PUBMED    32856047
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Aug 28. pii: 5897334. doi: 10.1093/nar/gkaa682."
COMMENT     Sequence Label: pEPKK0CM0062
FEATURES             Location/Qualifiers
     source          1..2175
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(40..628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(120..139)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(811..905)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(926..1585)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1500..1519
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     1747..1765
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1769..1812)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     protein_bind    1840..1873
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    1899..1932
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     terminator      1963..2020
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(2044..2061)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc
       61 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      121 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      181 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      241 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      301 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      361 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      421 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      481 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
      541 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
      601 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
      661 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
      721 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
      781 gtaaacttgg tctgacagct cgaggcttgg attctcacca ataaaaaacg cccggcggca
      841 accgagcgtt ctgaacaaat ccagatggag ttctgaggtc attactggat ctatcaacag
      901 gagtccaagc gagctcgata tcaaattacg ccccgccctg ccactcatcg cagtactgtt
      961 gtaattcatt aagcattctg ccgacatgga agccatcaca aacggcatga tgaacctgaa
     1021 tcgccagcgg catcagcacc ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg
     1081 gggcgaagaa gttgtccata ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg
     1141 gattggctga gacgaaaaac atattctcaa taaacccttt agggaaatag gccaggtttt
     1201 caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt
     1261 attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg taacaagggt
     1321 gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat tccggatgag
     1381 cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc ttatttttct
     1441 ttacggtctt taaaaaggcc gtaatatcca gctgaacggt ctggttatag gtacattgag
     1501 caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata tcaacggtgg
     1561 tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat ctcgataact
     1621 caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa cctcttacgt
     1681 gcccgatcaa ctcgagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     1741 tataaaaata ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct
     1801 aaggatgatt tctggaattc ggtctcagga gcggtgcggg tgccagggcg tgcccttggg
     1861 ctccccgggc gcgtactcca ctccccgagc cggtgcgggt gccagggcgt gcccttgggc
     1921 tccccgggcg cgtactccac tccccccatc gagaccctgc agtccggcaa aaaagggcaa
     1981 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg ttatgcaggc
     2041 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2101 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2161 agcaaaaggc cagca
//
