LOCUS       sequence-326574-        2699 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive murine mature BDNF expression, controlled under E.coli 
            J23101 (strong) promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Toparlak OD, Zasso J, Bridi S, Serra MD, Macchi P, Conti L, Baudet 
            ML, Mansy SS
  TITLE     Artificial cells drive neural differentiation.
  JOURNAL   Sci Adv. 2020 Sep 18;6(38). pii: 6/38/eabb4920. doi: 
            10.1126/sciadv.abb4920. Print 2020 Sep.
  PUBMED    32948587
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2020-09-18"; volume: "6(38). pii: 6/38/eabb4920. doi"; pages:
            " 10.1126/sciadv.abb4920. Print 2020 Sep"
COMMENT     Sequence Label: DT050A
FEATURES             Location/Qualifiers
     source          1..2699
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     279..298
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(517..621)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     689..707
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(711..754)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    757..776
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        778..812
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             841..864
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      1350..1397
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    1399..1419
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(1425..1442)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1596..2184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1676..1695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2355..2699,1..447,448..516))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
       61 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
      121 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
      181 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
      241 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
      301 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
      361 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
      421 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
      481 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
      541 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
      601 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
      661 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
      721 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagattt
      781 acagctagct cagtcctagg tattatgcta gcgatactag aagaaggaga ataatctatg
      841 gactacaaag acgatgatga caagatgcac tccgaccctg cccgccgtgg ggagctgagc
      901 gtgtgtgaca gtattagcga gtgggtcaca gcggcagata aaaagactgc agtggacatg
      961 tctggcggga cggtcacagt cctagagaaa gtcccggtat ccaaaggcca actgaagcag
     1021 tatttctacg agaccaagtg taatcccatg ggttacacca aggaaggctg caggggcata
     1081 gacaaaaggc actggaactc gcaatgccga actacccaat cgtatgttcg ggcccttact
     1141 atggatagca aaaagagaat tggctggcga ttcataagga tagacacttc ctgtgtatgt
     1201 acactgacca ttaagagggg aagatagtag cgactcaggc tgctacgcct gtgtactgga
     1261 aaacaaaacc aaaacccaaa aaacaaaaaa ctgagcccat tggtatcgtg gaaggactcg
     1321 atcaaaaaaa aaaaaaaaaa aaaaaaaaac tagcataacc ccttggggcc tctaaacggg
     1381 tcttgagggg ttttttggta ctagtagcgg ccgctgcagg cttcctcgct cactgactcg
     1441 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1501 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1561 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc acaggctccg cccccctgac
     1621 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1681 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1741 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1801 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1861 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1921 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     1981 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2041 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2101 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2161 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2221 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2281 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2341 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2401 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2461 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2521 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2581 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2641 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgtt
//
