LOCUS       sequence-326625-        8541 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ACX, rPB(8kD) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8541)
  AUTHORS   Chen L, Park JE, Paa P, Rajakumar PD, Prekop HT, Chew YT, Manivannan
            SN, Chew WL
  TITLE     Programmable C:G to G:C genome editing with CRISPR-Cas9-directed 
            base excision repair proteins.
  JOURNAL   Nat Commun. 2021 Mar 2;12(1):1384. doi: 10.1038/s41467-021-21559-9.
  PUBMED    33654077
REFERENCE   2  (bases 1 to 8541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-03-2"; volume: "12(1):1384. doi"; pages: " 
            10.1038/s41467-021-21559-9"
COMMENT     Sequence Label: pLD003-pCMV-APOBEC-Cas9(D10A)-rPB(8kD)
FEATURES             Location/Qualifiers
     source          1..8541
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             658..1344
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             join(1393..4791,4792..4794,4795..5391,5392,5393..5395,
                     5396..5397,5398..5400,5401..5493)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1393..1416,1417..1419,1420..5493)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5776..5796
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5805..5822
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5845..5862)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5851..6075
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6146..6162)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6146..6162)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6159..6181)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6170..6186
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6194..6200,6201..6218,6219..6224))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6239..6260
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6377..6394)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6548..7136)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6628..6647)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7307..8098,8099..8167))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7930..7949
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8168..8272)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8351..8370)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
      601 agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc cgccaccatg
      661 agctcagaga ctggcccagt ggctgtggac cccacattga gacggcggat cgagccccat
      721 gagtttgagg tattcttcga tccgagagag ctccgcaagg agacctgcct gctttacgaa
      781 attaattggg ggggccggca ctccatttgg cgacatacat cacagaacac taacaagcac
      841 gtcgaagtca acttcatcga gaagttcacg acagaaagat atttctgtcc gaacacaagg
      901 tgcagcatta cctggtttct cagctggagc ccatgcggcg aatgtagtag ggccatcact
      961 gaattcctgt caaggtatcc ccacgtcact ctgtttattt acatcgcaag gctgtaccac
     1021 cacgctgacc cccgcaatcg acaaggcctg cgggatttga tctcttcagg tgtgactatc
     1081 caaattatga ctgagcagga gtcaggatac tgctggagaa actttgtgaa ttatagcccg
     1141 agtaatgaag cccactggcc taggtatccc catctgtggg tacgactgta cgttcttgaa
     1201 ctgtactgca tcatactggg cctgcctcct tgtctcaaca ttctgagaag gaagcagcca
     1261 cagctgacat tctttaccat cgctcttcag tcttgtcatt accagcgact gcccccacac
     1321 attctctggg ccaccgggtt gaaaagcggc agcgagactc ccgggacctc agagtccgcc
     1381 acacccgaaa gtgataaaaa gtattctatt ggtttagcca tcggcactaa ttccgttgga
     1441 tgggctgtca taaccgatga atacaaagta ccttcaaaga aatttaaggt gttggggaac
     1501 acagaccgtc attcgattaa aaagaatctt atcggtgccc tcctattcga tagtggcgaa
     1561 acggcagagg cgactcgcct gaaacgaacc gctcggagaa ggtatacacg tcgcaagaac
     1621 cgaatatgtt acttacaaga aatttttagc aatgagatgg ccaaagttga cgattctttc
     1681 tttcaccgtt tggaagagtc cttccttgtc gaagaggaca agaaacatga acggcacccc
     1741 atctttggaa acatagtaga tgaggtggca tatcatgaaa agtacccaac gatttatcac
     1801 ctcagaaaaa agctagttga ctcaactgat aaagcggacc tgaggttaat ctacttggct
     1861 cttgcccata tgataaagtt ccgtgggcac tttctcattg agggtgatct aaatccggac
     1921 aactcggatg tcgacaaact gttcatccag ttagtacaaa cctataatca gttgtttgaa
     1981 gagaacccta taaatgcaag tggcgtggat gcgaaggcta ttcttagcgc ccgcctctct
     2041 aaatcccgac ggctagaaaa cctgatcgca caattacccg gagagaagaa aaatgggttg
     2101 ttcggtaacc ttatagcgct ctcactaggc ctgacaccaa attttaagtc gaacttcgac
     2161 ttagctgaag atgccaaatt gcagcttagt aaggacacgt acgatgacga tctcgacaat
     2221 ctactggcac aaattggaga tcagtatgcg gacttatttt tggctgccaa aaaccttagc
     2281 gatgcaatcc tcctatctga catactgaga gttaatactg agattaccaa ggcgccgtta
     2341 tccgcttcaa tgatcaaaag gtacgatgaa catcaccaag acttgacact tctcaaggcc
     2401 ctagtccgtc agcaactgcc tgagaaatat aaggaaatat tctttgatca gtcgaaaaac
     2461 gggtacgcag gttatattga cggcggagcg agtcaagagg aattctacaa gtttatcaaa
     2521 cccatattag agaagatgga tgggacggaa gagttgcttg taaaactcaa tcgcgaagat
     2581 ctactgcgaa agcagcggac tttcgacaac ggtagcattc cacatcaaat ccacttaggc
     2641 gaattgcatg ctatacttag aaggcaggag gatttttatc cgttcctcaa agacaatcgt
     2701 gaaaagattg agaaaatcct aacctttcgc ataccttact atgtgggacc cctggcccga
     2761 gggaactctc ggttcgcatg gatgacaaga aagtccgaag aaacgattac tccatggaat
     2821 tttgaggaag ttgtcgataa aggtgcgtca gctcaatcgt tcatcgagag gatgaccaac
     2881 tttgacaaga atttaccgaa cgaaaaagta ttgcctaagc acagtttact ttacgagtat
     2941 ttcacagtgt acaatgaact cacgaaagtt aagtatgtca ctgagggcat gcgtaaaccc
     3001 gcctttctaa gcggagaaca gaagaaagca atagtagatc tgttattcaa gaccaaccgc
     3061 aaagtgacag ttaagcaatt gaaagaggac tactttaaga aaattgaatg cttcgattct
     3121 gtcgagatct ccggggtaga agatcgattt aatgcgtcac ttggtacgta tcatgacctc
     3181 ctaaagataa ttaaagataa ggacttcctg gataacgaag agaatgaaga tatcttagaa
     3241 gatatagtgt tgactcttac cctctttgaa gatcgggaaa tgattgagga aagactaaaa
     3301 acatacgctc acctgttcga cgataaggtt atgaaacagt taaagaggcg tcgctatacg
     3361 ggctggggac gattgtcgcg gaaacttatc aacgggataa gagacaagca aagtggtaaa
     3421 actattctcg attttctaaa gagcgacggc ttcgccaata ggaactttat gcagctgatc
     3481 catgatgact ctttaacctt caaagaggat atacaaaagg cacaggtttc cggacaaggg
     3541 gactcattgc acgaacatat tgcgaatctt gctggttcgc cagccatcaa aaagggcata
     3601 ctccagacag tcaaagtagt ggatgagcta gttaaggtca tgggacgtca caaaccggaa
     3661 aacattgtaa tcgagatggc acgcgaaaat caaacgactc agaaggggca aaaaaacagt
     3721 cgagagcgga tgaagagaat agaagagggt attaaagaac tgggcagcca gatcttaaag
     3781 gagcatcctg tggaaaatac ccaattgcag aacgagaaac tttacctcta ttacctacaa
     3841 aatggaaggg acatgtatgt tgatcaggaa ctggacataa accgtttatc tgattacgac
     3901 gtcgatcaca ttgtacccca atcctttttg aaggacgatt caatcgacaa taaagtgctt
     3961 acacgctcgg ataagaaccg agggaaaagt gacaatgttc caagcgagga agtcgtaaag
     4021 aaaatgaaga actattggcg gcagctccta aatgcgaaac tgataacgca aagaaagttc
     4081 gataacttaa ctaaagctga gaggggtggc ttgtctgaac ttgacaaggc cggatttatt
     4141 aaacgtcagc tcgtggaaac ccgccaaatc acaaagcatg ttgcacagat actagattcc
     4201 cgaatgaata cgaaatacga cgagaacgat aagctgattc gggaagtcaa agtaatcact
     4261 ttaaagtcaa aattggtgtc ggacttcaga aaggattttc aattctataa agttagggag
     4321 ataaataact accaccatgc gcacgacgct tatcttaatg ccgtcgtagg gaccgcactc
     4381 attaagaaat acccgaagct agaaagtgag tttgtgtatg gtgattacaa agtttatgac
     4441 gtccgtaaga tgatcgcgaa aagcgaacag gagataggca aggctacagc caaatacttc
     4501 ttttattcta acattatgaa tttctttaag acggaaatca ctctggcaaa cggagagata
     4561 cgcaaacgac ctttaattga aaccaatggg gagacaggtg aaatcgtatg ggataagggc
     4621 cgggacttcg cgacggtgag aaaagttttg tccatgcccc aagtcaacat agtaaagaaa
     4681 actgaggtgc agaccggagg gttttcaaag gaatcgattc ttccaaaaag gaatagtgat
     4741 aagctcatcg ctcgtaaaaa ggactgggac ccgaaaaagt acggtggctt cgatagccct
     4801 acagttgcct attctgtcct agtagtggca aaagttgaga agggaaaatc caagaaactg
     4861 aagtcagtca aagaattatt ggggataacg attatggagc gctcgtcttt tgaaaagaac
     4921 cccatcgact tccttgaggc gaaaggttac aaggaagtaa aaaaggatct cataattaaa
     4981 ctaccaaagt atagtctgtt tgagttagaa aatggccgaa aacggatgtt ggctagcgcc
     5041 ggagagcttc aaaaggggaa cgaactcgca ctaccgtcta aatacgtgaa tttcctgtat
     5101 ttagcgtccc attacgagaa gttgaaaggt tcacctgaag ataacgaaca gaagcaactt
     5161 tttgttgagc agcacaaaca ttatctcgac gaaatcatag agcaaatttc ggaattcagt
     5221 aagagagtca tcctagctga tgccaatctg gacaaagtat taagcgcata caacaagcac
     5281 agggataaac ccatacgtga gcaggcggaa aatattatcc atttgtttac tcttaccaac
     5341 ctcggcgctc cagccgcatt caagtatttt gacacaacga tagatcgcaa acgatacact
     5401 tctaccaagg aggtgctaga cgcgacactg attcaccaat ccatcacggg attatatgaa
     5461 actcggatag atttgtcaca gcttgggggt gactctggtg gttctagcaa acgcaaggcc
     5521 ccgcaggaga ccctcaacgg cggcatcacg gacatgctcg tggaactcgc aaactttgag
     5581 aagaacgtga gccaggcgat ccacaagtac aatgcataca gaaaagcagc atctgtgata
     5641 gcaaagtacc cacacaaaat caagagtgga gcagaagcta agaaattgcc aggagtagga
     5701 acaaaaattg ctgaaaagat tgatgaattt ttagcaactg gaaaattgcg taaactggaa
     5761 aagtctggtg gttctcccaa gaagaagagg aaagtctaac cggtcatcat caccatcacc
     5821 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5881 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5941 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6001 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6061 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata ccgtcgacct
     6121 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6181 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag ggtgcctaat
     6241 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6301 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6361 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6421 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6481 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6541 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6601 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6661 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6721 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6781 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6841 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6901 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6961 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     7021 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7081 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7141 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7201 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7261 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7321 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7381 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7441 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7501 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7561 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7621 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7681 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7741 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7801 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7861 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7921 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7981 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8041 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8101 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8161 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8221 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8281 cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat cccctagggt
     8341 cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     8401 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct
     8461 tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
     8521 tacgggccag atatacgcgt t
//
