LOCUS       sequence-327070-       11228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of SARS-CoV-2 soluble spike trimer protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Esposito D, Mehalko J, Drew M, Snead K, Wall V, Taylor T, Frank P, 
            Denson JP, Hong M, Gulten G, Sadtler K, Messing S, Gillette W
  TITLE     Optimizing high-yield production of SARS-CoV-2 soluble spike trimers
            for serology assays.
  JOURNAL   Protein Expr Purif. 2020 Jun 4;174:105686. doi: 
            10.1016/j.pep.2020.105686.
  PUBMED    32504802
REFERENCE   2  (bases 1 to 11228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr Purif."; date: "2020-06-4"; volume: "174:105686. doi"; pages: "
            10.1016/j.pep.2020.105686"
COMMENT     Sequence Label: R619-M63-303: CMV51p> SARS-CoV-2
            S(1-1208)-2P-...
FEATURES             Location/Qualifiers
     source          1..11228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      204..1993
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(2030..2049)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     protein_bind    2199..2219
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2230..2533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2534..2737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2688..2708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2734..2758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2803..2827
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6550..6573
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6577..6600
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             6607..6630
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6670..6693
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(6695..6719)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6781..6799)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6782..6799)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6819..6836)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6825..7049
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7095..7523
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7182..7201)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7392..7413
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7532..7552)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7537..7866
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7717..7852
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7779..7798
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7933..8727
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7987..8006)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8597..8616
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8901..9022
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8938..8957)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8992..9011
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9071..9087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9071..9087)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9084..9106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9095..9111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9119..9125,9126..9143,9144..9149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9164..9185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9302..9319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9473..10061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9553..9572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10232..11023,11024..11092))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10855..10874
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11093..11197)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatccgcc tttatgtgta actcttggct gaagctctta caccaatgct
       61 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
      121 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
      181 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
      241 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
      301 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
      361 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
      421 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
      481 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
      541 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
      601 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
      661 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
      721 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
      781 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
      841 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
      901 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
      961 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     1021 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     1081 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     1141 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     1201 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     1261 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     1321 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     1381 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     1441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     1501 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     1561 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     1621 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1681 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     1741 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     1801 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     1861 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     1921 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     1981 tgtccttttc ctgctgggga tctcccgatc ccctatggtg cactctcagt acaatctgct
     2041 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     2101 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     2161 atctgcttag ggttaggcgt tttgcgctgc ttcgatcaca actttgtata gaaaagttgg
     2221 ccctgcaggc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     2281 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     2341 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     2401 tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     2461 tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg tattagtcat
     2521 cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat agcggtttga
     2581 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     2641 aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg
     2701 taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc gtcagatcgc
     2761 ctggagacgc catccacgct gttttgacct ccatagaaga caacaacttt gtacaaaaaa
     2821 gttggcacca tgttcgtgtt ccttgtcctc ctgccgctgg tgtcatcgca gtgcgtgaac
     2881 ctgaccaccc ggactcagct gcctcctgct tacactaact ccttcactcg cggggtgtac
     2941 taccctgaca aagtgttccg gtcgtccgtg ctgcatagca cccaggacct gttccttccg
     3001 ttcttctcca acgtgacctg gttccacgcc attcacgtgt cggggaccaa cggcactaag
     3061 agattcgaca accccgtctt gcccttcaat gacggagtat acttcgcctc gacggaaaag
     3121 tccaacatca ttcggggttg gatcttcggc actaccctgg acagcaagac ccagtccctg
     3181 ctcattgtca acaacgccac caacgtcgtg atcaaagtct gcgagtttca gttttgtaac
     3241 gatccgttcc tcggcgtgta ctaccacaag aacaacaaga gctggatgga gtccgaattc
     3301 cgggtctact cctccgccaa caactgtacc ttcgaatacg tgtcccaacc tttcctcatg
     3361 gacctcgaag gaaaacaggg aaacttcaag aacttgagag agtttgtgtt caagaatatc
     3421 gatggctact tcaagatcta ctcgaagcat accccgatca acctcgtgcg ggacctacct
     3481 caagggttct ccgcacttga gccgctcgtg gacctcccca ttggcatcaa catcaccaga
     3541 ttccagactt tactggcact gcatagatcg tatctcacac ccggggactc ctcctccggg
     3601 tggacagctg gtgctgcggc ctactacgtg ggatatctcc agccccggac cttcttgctg
     3661 aagtacaacg agaacggcac catcacggat gccgtggact gtgcactgga ccccttgagt
     3721 gaaaccaagt gcacgctgaa gtccttcacc gtggaaaaag gcatctacca gaccagcaac
     3781 ttcagggtgc agcctactga atcaatcgtg cggttcccta acattaccaa tctgtgcccg
     3841 ttcggcgaag tgttcaacgc cactagattc gcctcggtgt acgcctggaa ccgaaagcgg
     3901 atcagcaact gcgtggccga ctactcagtg ctctacaact cggccagctt ctccaccttc
     3961 aagtgctacg gagtgtcgcc caccaagctg aacgatctgt gcttcaccaa cgtgtacgcg
     4021 gattccttcg tgatccgcgg agatgaagtc cgccaaatcg cccccggtca aaccggaaag
     4081 attgccgact acaactataa gctgccggac gactttaccg gatgcgtgat tgcctggaat
     4141 tccaacaatt tggattcaaa agtcggcggc aactacaact acctgtaccg cctgttccgg
     4201 aagtcaaacc tgaagccgtt cgaacgcgac atcagcaccg aaatctatca agcgggaagc
     4261 actccctgca acggggtgga gggcttcaac tgctacttcc cgctgcaaag ctacggattc
     4321 cagcctacca acggcgttgg ataccagcca tatcgcgtgg tcgtgctctc ctttgaactt
     4381 ctgcacgccc ctgccactgt gtgcggcccc aagaagtcca ctaacctcgt caagaataag
     4441 tgcgtgaatt tcaactttaa cggattgacc gggaccggag tgctcaccga gtccaacaag
     4501 aaattcctgc cgttccaaca gtttggacgg gacatcgccg atactacgga tgctgtgcgg
     4561 gacccacaga ctctggagat cctggacatt actccctgtt cctttggcgg ggtgtcggtc
     4621 atcacccctg gtactaacac cagcaaccag gtcgccgtgc tgtatcagga cgtgaactgc
     4681 accgaagtgc ccgtggcgat ccacgccgac cagctgactc caacttggcg cgtgtactcg
     4741 actggttcga acgtgttcca gactcgggcc ggttgcctga tcggagcgga gcatgtcaac
     4801 aacagctacg agtgcgacat ccctattgga gccgggattt gcgccagcta ccagacccag
     4861 accaattcgc ctgggtcggc cagcagcgtg gcttcccaat cgatcatcgc ctacaccatg
     4921 tccctgggag ccgagaactc agtggcgtac tccaacaact cgattgccat ccccactaat
     4981 ttcacgatca gcgtgaccac cgagatcctg cctgtgtcca tgaccaagac ctccgtggac
     5041 tgcaccatgt acatctgcgg agactccacc gagtgctcca acctcctgct gcaatacggc
     5101 tcgttctgta cccaactgaa ccgagccctg actgggatcg ccgtggagca ggacaagaac
     5161 acacaggagg tgttcgcaca agtcaagcag atctacaaga ccccgcccat caaggatttc
     5221 ggagggttca atttctccca aattctgccg gacccttcca agccgtccaa gaggtcattc
     5281 attgaggatc tcctgttcaa caaggtcacc ctggccgatg ctggtttcat caagcagtac
     5341 ggagactgcc tgggggacat cgcagcccgc gatctgatct gcgcccaaaa gttcaacggc
     5401 ctgaccgtcc tgcctccgct tctgactgat gaaatgattg cccagtacac ctctgcgctg
     5461 ctggctggca ctatcacctc cggctggacc ttcggcgctg gagcagcact gcagattcca
     5521 tttgccatgc aaatggccta ccgcttcaac ggaatcggag tgactcagaa cgtcctgtac
     5581 gagaaccaga agctcatcgc caaccagttc aactccgcca ttgggaagat tcaggattcc
     5641 ctgtcgagca ctgcgtcagc cctgggaaag ctgcaggacg tcgtgaacca gaacgcacag
     5701 gccctgaaca cccttgtgaa gcagctgtcg tcgaacttcg gggcgatctc ctccgtgttg
     5761 aacgacatcc tgtcccggct ggacccgcct gaggcagaag tgcagatcga ccggctgatt
     5821 accggacgcc tccagtccct tcaaacttat gtgacccagc agctgattcg cgcggcggaa
     5881 attcgggcta gcgccaatct ggctgccacc aagatgagcg aatgcgtgct tggacagtcc
     5941 aagagagtgg acttttgtgg aaagggctac cacctgatga gcttccccca atccgcccca
     6001 cacggtgtcg tgtttctgca cgtgacctac gtgccggcgc aggaaaagaa cttcaccact
     6061 gcccctgcca tctgccacga tgggaaggcg cacttcccga gggagggagt gttcgtgtca
     6121 aacggaaccc actggttcgt gacccaaagg aacttctacg agccccagat tatcaccacc
     6181 gataacactt tcgtgagcgg caactgcgac gtggtcatcg gcatcgtgaa caacactgtg
     6241 tacgacccgc tgcaaccgga gctcgatagc tttaaggaag aactcgacaa gtactttaag
     6301 aatcatacgt cccccgatgt ggatctgggc gacattagcg gtatcaacgc gtctgtcgtg
     6361 aacattcaga aggagattga caggctcaac gaagtcgcca aaaacctgaa cgagagcctg
     6421 atagacttgc aggaactggg aaaatacgag cagggctccg gttacatccc cgaagctcca
     6481 cgggacggac aggcctacgt gcgcaaggac ggagaatggg tgctgctgag caccttcctc
     6541 gggcggtccc tggaggtgct gttccaaggc ccaggccacc atcatcatca ccaccaccac
     6601 tcggcttggt cacaccccca attcgaaaag ggcggtggtt ctggaggagg cggatccgga
     6661 ggatccgcgt ggagtcatcc gcagtttgaa aagtaatcaa ctttcttgta caaagttgtg
     6721 aaattctgca gatatccatc acactggcgg ccgctcgagc atgcatctag agggccctat
     6781 tctatagtgt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     6841 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     6901 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     6961 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     7021 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     7081 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     7141 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     7201 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     7261 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     7321 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     7381 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     7441 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     7501 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
     7561 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     7621 aggtgtggaa agtccctagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7681 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7741 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7801 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     7861 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
     7921 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     7981 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     8041 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     8101 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     8161 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     8221 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     8281 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     8341 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     8401 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     8461 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     8521 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     8581 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     8641 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     8701 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
     8761 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
     8821 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
     8881 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
     8941 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     9001 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
     9061 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9121 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9181 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     9241 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     9301 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     9361 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     9421 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     9481 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9541 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9601 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9661 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9721 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9781 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9841 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9901 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9961 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
    10021 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
    10081 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
    10141 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
    10201 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
    10261 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
    10321 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
    10381 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
    10441 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
    10501 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10561 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10621 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10681 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10741 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10801 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10861 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10921 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10981 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    11041 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    11101 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    11161 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    11221 ctgacgtc
//
