LOCUS       sequence-327094-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Actin filament reporter, LifeAct, fused with blue fluorescent 
            protein TagBFP and fluorogen activating protein (FAP) DiB-RM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Bozhanova NG, Harp JM, Bender BJ, Gavrikov AS, Gorbachev DA, Baranov
            MS, Mercado CB, Zhang X, Lukyanov KA, Mishin AS, Meiler J
  TITLE     Computational redesign of a fluorogen activating protein with 
            Rosetta.
  JOURNAL   PLoS Comput Biol. 2021 Nov 8;17(11):e1009555. doi: 
            10.1371/journal.pcbi.1009555. eCollection 2021 Nov.
  PUBMED    34748541
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Comput
            Biol."; date: "2021-11-8"; volume: "17(11):e1009555. doi"; pages: " 
            10.1371/journal.pcbi.1009555. eCollection 2021 Nov"
COMMENT     Sequence Label: LifeAct-TagBFP-DiB-RM
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             40..90
                     /codon_start=1
                     /product="peptide that binds filamentous actin (Riedl et 
                     al., 2008)"
                     /label=Lifeact
                     /note="first 17 amino acids of budding yeast Abp140"
                     /translation="MGVADLIKKFESISKEE"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcgccacca tgggcgtggc cgacttgatc
       61 aagaagttcg agtccatctc caaggaggag ggggatccac cggtcgccac catgagcgag
      121 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga caaccatcac
      181 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
      241 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac tagcttcctc
      301 tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt caagcagtcc
      361 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
      421 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
      481 gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccttc
      541 accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat ggccctgaag
      601 ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc caagaaaccc
      661 gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga aagaatcaag
      721 gagg
//
