LOCUS       sequence-327189-        4614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish kcnn2 gene CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Silic MR, Black MM, Zhang G
  TITLE     Phylogenetic and developmental analyses indicate complex functions 
            of Calcium-Activated Potassium Channels in zebrafish embryonic 
            development.
  JOURNAL   Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.329.
  PUBMED    33728688
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2021 Mar 16. doi: 10.1002/dvdy.329."
COMMENT     Sequence Label: pENTR-D-TOPO-kcnn2
FEATURES             Location/Qualifiers
     source          1..4614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..46
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(205..232)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(324..410)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     459..481
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     473..490
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     474..490
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    506..605
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             899..922
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     protein_bind    complement(2676..2775)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2792..2811)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2793..2811)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2816..2832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2816..2832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2934..2953)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2945..3754
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3012..3031)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3901..4489
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4390..4409
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
       61 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      121 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatacgc gtaccgctag
      181 ccaggaagag tttgtagaaa cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt
      241 tgatgcctgg cagtttatgg cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca
      301 acgttcaaat ccgctcccgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa
      361 cagataaaac gaaaggccca gtcttccgac tgagcctttc gttttatttg atgcctggca
      421 gttccctact ctcgcgttaa cgctagcatg gatgttttcc cagtcacgac gttgtaaaac
      481 gacggccagt cttaagctcg ggccccaaat aatgatttta ttttgactga tagtgacctg
      541 ttcgttgcaa caaattgatg agcaatgctt ttttataatg ccaactttgt acaaaaaagc
      601 aggctccgcg gccgccccct tcaccatgga aaccccattg cagttgcaga acaatttcta
      661 tccagagcag cagcagcagc agcactgtaa gtaccagcac tactgcgggc gggaggaaga
      721 gcgctccatc aggcactgca cctgcaacaa ctcctccacc tgcgaggatg ccagaaaaag
      781 ccagcctttg cacgggacgc cgctgggaac tttaaggaca ccgtccgtct catcttcaac
      841 aaggcaaggc gctcagtaca gcgagttcac gccctctagt catggtagtt catccagaca
      901 tcatcatcac caccatcatc atcctcatcg cgaccacaac cggcagcaga ttcggggcag
      961 tccggccagc agccacagag agagtaactc ttacacccaa atagccatga gcagctgcag
     1021 atacaacggc ggcgtgatgc ggccgctcag caacttgagc tcgtcccgca ggaacctgca
     1081 cgagtttgat tcggagtccc agccactgca gcccgtctct gcggcggacg cgtccgacac
     1141 actcgcctcc aagccggaga acaactccag caccctcatg ccttacgcca cgggggacgg
     1201 aggaggagga ggtggaggcg gtggatgtag ccacagcggc agcaaatcgg gcaagaagaa
     1261 gaaccagaac atcgggcaga agctcgggca ccggagggcc ctgttcgaga agaggaagcg
     1321 gctcagcgac tacgcgctca tctttgggat gtttgggatc gtggtgatgg tcattgaaac
     1381 tgaactctct tggggagcct acggaaagga gtcactttat tctttagccc taaaatgcct
     1441 gataagcctt tctacaatca ttctacttgg gttgattatc atatatcatg ccagggaaat
     1501 ccagttattc atggtggaca acgccgcaga cgactggagg atagccatga cctatgagcg
     1561 catcttcttc atctgtctgg agatccttgt gtgcgccata caccccatcc caggcaacta
     1621 cactttcaca tggacggcgc ggcttgcctt ttcctacgca ccatccaaaa cagacgcaga
     1681 tgtggacatc atcctgtcca tccccatgtt tctgcgattg tacctcattg ctcgcgtcat
     1741 gttgctgcat agcaaactct tcaccgatgc ttcatcacgc agcatcggtg ctctcaacaa
     1801 aataaacttc aacacacgat ttgttatgaa gaccctcatg actatctgtc ccggcacagt
     1861 gttgctggtc ttcaccattt ctctgtggat catcgccgcc tggactgtga gggcctgcga
     1921 aagatatcat gacaacatgg atgtaaccag caatttcctt ggagccatgt ggttgatctc
     1981 aataaccttt ctaaccattg gatatgggga tatggttccc aatacatact gtggaaaagg
     2041 agtctgtctc ctcactggca tcatgggtgc cgggtgcact gctttggtgg tcgctgtggt
     2101 cgcaaaaaaa ctggaattaa ccaaagctga aaaacatgta cataacttta tgatggacac
     2161 ccagctgaca aaaagggtga aaaacacagc tgctaatgtt ctcagggaga cgtggctcat
     2221 ttataaaaac accaagctgg tgagaaagat ggaccacgcc aaagtcagga aacatcagcg
     2281 caagttcctt caagccattc accaagctcg aaaattaaga agtgtaaaaa tggagcaacg
     2341 caagctgaat gaccaagcaa actctctagt ggaccttgca aagactcaaa acgtcatgta
     2401 cgacctggtc tcggacctga acgagcgagg cgaggacatg gaaaagagga ttgcactgct
     2461 ggagaccaaa cttgagacct tgctcagcaa cttacaggca ctcccgggcc tcatcagtca
     2521 ggtgatcagc cagcaacacc gggacttcct ggaggtgcaa ctccagccat acgacaaaca
     2581 cagccacgag cgctcgcagt ctgtctcccg gcgcagatcg tcttccaccg cgcctcccac
     2641 ttcatccgag agcagctaga agggtgggcg cgccgaccca gctttcttgt acaaagttgg
     2701 cattataaga aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa
     2761 taaaatcatt atttgccatc cagctgatat cccctatagt gagtcgtatt acatggtcat
     2821 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
     2881 taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta atacaagggg
     2941 tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca acatggatgc
     3001 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     3061 tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     3121 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     3181 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     3241 ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     3301 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     3361 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     3421 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     3481 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     3541 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     3601 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     3661 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     3721 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     3781 ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa aatcccttaa
     3841 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3901 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3961 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     4021 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4081 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4141 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4201 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4261 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4321 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4381 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4441 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4501 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4561 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg atac
//
