LOCUS       sequence-327605-        8560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive expression (EF1a promoter) of GFP cDNA plus 
            miRE-embedded shRenilla (control shRNA), Puromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8560)
  TITLE     Karreth lab plasmids
REFERENCE   2  (bases 1 to 8560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti-EF1a-GFP-miRE_shRen-Puro
FEATURES             Location/Qualifiers
     source          1..8560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     179..198
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     438..459
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      501..510
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(530..741)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_feature    748..864
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1131..1175)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1360..1593
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2086..2211
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             2258..2438
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(2439..2665)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(2693..2713)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2693..2711)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2732..2748)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2732..2748)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2745..2767)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2756..2772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2780..2786,2787..2804,2805..2810))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2825..2846
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2963..2980)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3134..3722)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3214..3233)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3893..4684,4685..4753))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4516..4535
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4754..4858)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4884..5339)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5021..5042)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5233..5252
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5466..5488
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5480..5497
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5481..5497
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5507..5526
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5507..5525
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      5546..5681
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5721..5842
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5732..5751)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5786..5805
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             5914..6147
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     CDS             complement(6284..6883)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6367..6387)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     6864..6883
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     complement(6952..6971)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     7310..7333
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    7381..7969
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7487..7498
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7896..7916
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     ncRNA           complement(8137..8151)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     CDS             complement(join(8345..8560,1..498,499..501,502..504))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8348..8560,1..498,499..501,502..504))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8390..8411)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg
       61 gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat
      121 gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt
      181 cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat
      241 ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac
      301 gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca
      361 gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct
      421 gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa
      481 cagctcctcg cccttgctca ccatggtggc gaccggtgga tcctctagac tgtgttctgg
      541 cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc
      601 ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc
      661 gccaccttct ctaggcaccg gatcaattgc cgacccctcc ccccaacttc tcggggactg
      721 tgggcgatgt gcgctctgcc catcgataaa attttgaatt ttgtaatttg tttttgtaat
      781 tctttagttt gtatgtctgt tgctattatg tctactattc tttcccctgc actgtacccc
      841 ccaatccccc cttttctttt aaaagttaac cgataccgtc gagatccgtt cactaatcga
      901 atggatctgt ctctgtctct ctctccacct tcttcttcta ttccttcggg cctgtcgggt
      961 cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg cctaactcta
     1021 ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc tactatcatt
     1081 atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca caaacttgcc
     1141 catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg cgattcttca
     1201 attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc catccaggtc
     1261 gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca aggcacagca
     1321 gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg atcctttagg
     1381 tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac tgtgagttgc
     1441 aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg cactatacca
     1501 gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat agtgcttcct
     1561 gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt ttctctctgc
     1621 accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt tactacttta
     1681 tatttatata attcacttct ccaattgtcc ctcatatctc ctcctccagg tctgaagatc
     1741 agcggccgct tgctgtgcgg tggtcttact tttgttttgc tcttcctcta tcttgtctaa
     1801 agcttccttg gtgtctttta tctctatcct ttgatgcaca caatagaggg ttgctactgt
     1861 attatataat gatctaagtt cttctgatcc tgtctgaagg gatggttgta gctgtcccag
     1921 tatttgtcta cagccttctg atgtttctaa caggccagga ttaactgcga atcgttctag
     1981 ctccctgctt gcccatacta tatgttttaa tttatatttt ttctttcccc ctggccttaa
     2041 ccgaattttt tcccatcgcg atctaattct cccccgctta atactgacgc tctcgcaccc
     2101 atctctctcc ttctagcctc cgctagtcaa aatttttggc gtactcacca gtcgccgccc
     2161 ctcgcctctt gccgtgcgcg cttcagcaag ccgagtcctg cgtcgagaga gctcctctgg
     2221 tttccctttc gctttcaagt ccctgttcgg gcgccactgc tagagatttt ccacactgac
     2281 taaaagggtc tgagggatct ctagttacca gagtcacaca acagacgggc acacactact
     2341 tgaagcactc aaggcaagct ttattgaggc ttaagcagtg ggttccctag ttagccagag
     2401 agctcccagg ctcagatctg gtctaaccag agagacccgt ttatgtatcg agctaggcac
     2461 ttaaatacaa tatctctgca atgcggcaat tcagtggttc gtccaatcca tgtcagaccc
     2521 gtctgttgcc ttcctaataa ggcacgatcg taccacctta cttccaccaa tcggcatgca
     2581 cggtgctttt tctctccttg taaggcatgt tgctaactca tcgttaccat gttgcaagac
     2641 tacaagagta ttgcataaga ctacattaag cttgcagctc cagcttttgt tccctttagt
     2701 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     2761 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     2821 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     2881 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2941 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3001 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3061 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3121 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3181 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3241 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3301 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3361 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3421 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3481 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3541 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3601 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3661 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3721 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3781 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3841 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3901 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3961 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4021 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4081 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4141 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4201 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4261 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4321 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4381 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4441 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4501 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4561 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4621 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4681 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4741 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4801 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4861 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     4921 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4981 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     5041 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     5101 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     5161 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     5221 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     5281 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     5341 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     5401 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     5461 gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat acgactcact
     5521 atagggcgaa ttgggtacgt ccctaggcct ccaaaaaagc ctcctcacta cttctggaat
     5581 agctcagagg ccgaggcggc ctcggcctct gcataaataa aaaaaattag tcagccatgg
     5641 ggcggagaat gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg
     5701 atagctagag ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     5761 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     5821 tataagctgc aataaacaag ttcctctcac tctctgatat tcatttcttt gcaagttata
     5881 aatactgaat aataagatga catgaactac tactgctaga gattttccac actgactaaa
     5941 agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac actacttgaa
     6001 gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag ccagagagct
     6061 cccaggctca gatctggtct aaccagagag acccagtaca agcaaaaagc agatcttgtc
     6121 ttcgttggga gtgagctagc ccttccagtc cccccttttc ttttaaaaag tggctaagat
     6181 ctacagctgc cttgtaagtc attggtctta aaggtaccgg gccctcgaat gcatggggtc
     6241 gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca ccgggcttgc
     6301 gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg acggtgaagc
     6361 cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag gcgggcaccc
     6421 cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga cccttgccct
     6481 ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg ggccggtgcg
     6541 gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc aactcggcca
     6601 tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc gtggtccaga
     6661 ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc ccgacgcgcg
     6721 tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga tcgacggtgt
     6781 ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc ctggggacgt
     6841 cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc ttgggctgca
     6901 ggtcgaaagg cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag
     6961 cgtgcagaat gccgggcctc cggaggacct tcgggcgccc gccccgcccc tgagcccgcc
     7021 cctgagccca gaaagcgaag gagcaaagct gctattggcc gctgccccaa aggcctaccc
     7081 gcttccattg ctcagcggtg ctgtccatct gcacgagact agtgagacgt gctacttcca
     7141 tttgtcacgt cctgcacgac gcgagctgcg gggcgggggg gaacttcctg actaggggag
     7201 gagtagaagg tggcgcgaag gggccaccaa agaacggagc cggttggcgc ctaccggtgg
     7261 atgtggaatg tgtgcgaggc cagaggccac ttgtgtagcg ccaagtgccc agcggggctg
     7321 ctaaagcgca tgctccagac tgccttggga aaagcgcctc ccctacccgg tagaatccag
     7381 gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa
     7441 gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag
     7501 ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccatg
     7561 gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
     7621 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
     7681 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
     7741 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
     7801 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
     7861 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
     7921 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg tcgacattga
     7981 aaaaagtgat ttaatttata ccattttaat tcagctttgt aaaaatgtat caaagagata
     8041 gcaaggtatt cagttttagt aaacaagata attgctcgaa ttctagcccc ttgaagtccg
     8101 aggcagtagg cataggaatt ataatgctta tctatacatc tgtggcttca ctatagataa
     8161 gcattataat tcctgcgctc actgtcaaca gcaatatacc ttctcgagcc ttctgttggg
     8221 ttaagaagtc gaagtaatcc cagcaagtgt ttccaagatg tgcaggcaac gattctgtaa
     8281 agtactgaag cctcattcaa acaattaccc tgttatccct agtcgaggcg gccgccgtac
     8341 gtcattactt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
     8401 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
     8461 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
     8521 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg
//
