LOCUS       sequence-327607-         446 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding a placeholder sequence as a Type 234 part to be 
            used in the Dueber YTK system (MoClo kit).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Camara E, Lenitz I, Nygard Y
  TITLE     A CRISPR activation and interference toolkit for industrial 
            Saccharomyces cerevisiae strain KE6-12.
  JOURNAL   Sci Rep. 2020 Sep 3;10(1):14605. doi: 10.1038/s41598-020-71648-w.
  PUBMED    32884066
REFERENCE   2  (bases 1 to 446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-09-3"; volume: "10(1):14605. doi"; pages: " 
            10.1038/s41598-020-71648-w"
COMMENT     Sequence Label: EC0_6_sgRNA
FEATURES             Location/Qualifiers
     source          1..446
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        292..367
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 atgtgcttca gtattacatt ttttgccttc aacgccttga ttgttctatt tttgctaata
       61 ataaatctat ttcatcggac taaaagtcca ttagttgtaa gcggatttag ctcagttggg
      121 agagcgccag actgaagaaa aacttcggtc aagtcatctg gaggtcctgt gttcgatcca
      181 cagaattcgc agatggccgg catggtccca gcctcctcgc tggcgccggc tgggcaacac
      241 cttcgggtgg cgaatgggac tcgctatcgt cttctcacga agacttactc agttttagag
      301 ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
      361 tcggtgcttt ttttattttt tgtcactatt gttatgtaaa atgccacctc tgacagtatg
      421 gaacgcaaac ttctgtctag tggata
//
