LOCUS       sequence-32769-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Xu W, Lipscombe D
  TITLE     Neuronal Ca(V)1.3alpha(1) L-type channels activate at relatively 
            hyperpolarized membrane potentials and are incompletely inhibited by
            dihydropyridines.
  JOURNAL   J Neurosci. 2001 Aug 15. 21(16):5944-51.
  PUBMED    11487617
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Neurosci.
            2001 Aug 15. 21(16):5944-51."
COMMENT     Sequence Label: CaV1.3e[8a,11,31b,Delta-32,42a] mut
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..20)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(30..71)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     107..123
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     107..123
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 tcatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag agggttaggg
       61 ataggcttac cttcgaaccg cgggccctct agactcgagc ggccgctcta gaactagtgg
      121 atcccccggg ctgcaggaat tcggcacgag ggcgtttccc cgcggacacg gtaggggaga
      181 cggaacctga gctgttgcct ggttggtttt ggcatgcggg tgtatgtgtt agtttcaatg
      241 ggtgtgtctg agcttcctgg ccttccacct acagtgcatt ctggactagg tcactgcagc
      301 aagcagggcc gggtggtcag gaggttgtga cgtgctggca caggaaagga actctgtctg
      361 ctcagttcca atcagcctgc cagtctttgg gagtccgggc aaactaggga tgcaggactt
      421 tggggcagag tccgttgtct gccttacaca ggtctgctta gtcatatata tatacataca
      481 catatatatg tatatatata tatattaaaa acattattta ttttattcac tggccatcgt
      541 cagcaagttg tgttcatttg atttctctgt ggggtggctg cccctttgcg ttctctctct
      601 ccctctctcc ctccctctct ctctttcttt ccctctcact gagtctctgg cttggaaggg
      661 gaaaacataa ctcatatgtt ccctttcatg aaaaggagcc accatgagca gtgtccgcaa
      721 caggcagcca gggctccaca gatgagccac acacagcccg gcttctgcct tccttccttc
      781 cttccggagg agtgccttgc tcgcaagggg tctccccagg gctttggtgc ctgcatttct
      841 cctgaaatgc tgcttcagta
//
