LOCUS       sequence-327829-        6857 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human TXLNA mammalian expression vector with C-terminal HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Li S, Wang L, Berman M, Kong YY, Dorf ME
  TITLE     Mapping a dynamic innate immunity protein interaction network 
            regulating type I interferon production.
  JOURNAL   Immunity. 2011 Sep 23;35(3):426-40. Epub 2011 Sep 8.
  PUBMED    21903422
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity.";
            date: "2011-09-23"; volume: "35(3)"; pages: "426-40. Epub 2011 Sep 
            8"
COMMENT     Sequence Label: pCMV3Tag8li-hTXLNA-HA
FEATURES             Location/Qualifiers
     source          1..6857
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        148..451
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        452..655
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     606..626
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        701..719
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             2395..2421
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2446..2517
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     primer_bind     complement(2570..2589)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2571..2589)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2618..2635)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2624..2831
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(2838..3293)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2975..2996)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3187..3206
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3320..3424
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3425..3493,3494..4282)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3643..3662)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        4376..4521
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             4564..5601
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(5599..5618)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5643..5691
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6242..6830
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6731..6750
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
       61 gtggataacc gtattaccgc catgcattag ttattaatag taatcaatta cggggtcatt
      121 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      181 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
      241 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      301 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      361 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      421 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      481 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      541 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      601 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
      661 agtgaaccgt cagatccgct agcgattacg ccaagctcga aattaaccct cactaaaggg
      721 aacaaaagct ggctcgagac catgaagaac caagacaaaa agaacggggc tgccaaacaa
      781 tccaatccaa aaagcagccc aggacaaccg gaagcaggac ccgagggagc ccaggagcgg
      841 cccagccagg cggctcctgc agtagaagca gaaggtcccg gcagcagcca ggctcctcgg
      901 aagccggagg gggctcaagc cagaacggct cagtctgggg cccttcgtga tgtctctgag
      961 gagctgagcc gccaactgga agacatactg agcacatact gtgtggacaa taaccagggg
     1021 ggccccggcg aggatggggc acagggtgag ccggctgaac ccgaagatgc agagaagtcc
     1081 cggacctatg tggcaaggaa tggggagcct gaaccaactc cagtagtcaa tggagagaag
     1141 gaaccctcca agggggatcc aaacacagaa gagatccggc agagtgacga ggtcggagac
     1201 cgagaccatc gaaggccaca ggagaagaaa aaagccaagg gtttggggaa ggagatcacg
     1261 ttgctgatgc agacattgaa tactctgagt accccagagg agaagctggc tgctctgtgc
     1321 aagaagtatg ctgaactgct ggaggagcac cggaattcac agaagcagat gaagctccta
     1381 cagaaaaagc agagccagct ggtgcaagag aaggaccacc tgtgcggtga gcacagcaag
     1441 gccgtcctgg cccgcagcaa gcttgagagc ctatgccgtg agctgcagcg gcacaaccgc
     1501 tccctcaagg aagaaggtgt gcagcgggcc cgggaggagg aggagaagcg caaggaggtg
     1561 acctcgcact tccaggtgac actgaatgac attcagctgc agatggaaca gcacaatgag
     1621 cgcaactcca agctgcgcca agagaacatg gagctggctg agaggctcaa gaagctgatt
     1681 gagcagtatg agctgcgcga ggagcatatc gacaaagtct tcaaacacaa ggacctacaa
     1741 cagcagctgg tggatgccaa gctccagcag gcccaggaga tgctaaagga ggcagaagag
     1801 cggcaccagc gggagaagga ttttctcctg aaagaggcag tagagtccca gaggatgtgt
     1861 gagctgatga agcagcaaga gacccacctg aagcaacagc ttgccctata cacagagaag
     1921 tttgaggagt tccagaacac actttccaaa agcagcgagg tattcaccac attcaagcag
     1981 gagatggaaa agatgactaa gaagatcaag aagctggaga aagaaaccac catgtaccgg
     2041 tcccggtggg agagcagcaa caaggccctg cttgagatgg ctgaggagaa aacagtccgg
     2101 gataaagaac tggagggcct gcaggtaaaa atccaacggc tggagaagct gtgccgggca
     2161 ctgcagacag agcgcaatga cctgaacaag agggtacagg acctgagtgc tggtggccag
     2221 ggctccctca ctgacagtgg ccctgagagg aggccagagg ggcctggggc tcaagcaccc
     2281 agctccccca gggtcacaga agcgccttgc tacccaggag caccgagcac agaagcatca
     2341 ggccagactg ggcctcaaga gcccacctcc gccagggccg cggccgcaat cgattaccca
     2401 tacgatgttc cagattacgc tctttaggaa ttcttcgatg tcgaggatta caaggatgac
     2461 gacgataagg actataagga cgatgatgac aaggactaca aagatgatga cgataaatag
     2521 ggcccggtac cttaattaat taaggtacca ggtaagtgta cccaattcgc cctatagtga
     2581 gtcgtattac aattcactcg atcggctcgc tgatcagcct cgactgtgcc ttctagttgc
     2641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2821 catgctgggg aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     2881 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     2941 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3001 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3061 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3121 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3181 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3241 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     3301 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3361 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3421 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3481 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3541 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3601 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     3661 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     3721 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     3781 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     3841 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     3901 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     3961 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4021 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4081 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4141 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4201 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4261 tgcctcactg attaagcatt ggcctcagga ttagtgcttc atccccgtgg cccgttgctc
     4321 gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt ctatgatgac
     4381 acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg
     4441 gcgcggtccg aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg
     4501 accctgcagc gacccgctta acagcgtcaa cagcgtgccg cagatcccgg ggggcaatga
     4561 gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     4621 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     4681 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     4741 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     4801 attggggaat tcagcgagag cctgacctat tgcatctccc gccgtgcaca gggtgtcacg
     4861 ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc agccggtcgc ggaggccatg
     4921 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     4981 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     5041 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     5101 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     5161 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     5221 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     5281 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     5341 ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     5401 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     5461 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     5521 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccggatcgg
     5581 gagatggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     5641 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     5701 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     5761 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     5821 tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg ggttagggac
     5881 ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg gcgttgcgtg
     5941 gggtcaggtc cacgactgga ctgagcagac agacccatgg tttttggatg gcctgggcat
     6001 ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa acacccccga
     6061 cccccaaaaa ccaccgcgcg gatttctggc gccgccggac gaactaaacc tgactacgac
     6121 atctctgccc cttcttcgct ggtacgagga gcgctttgcc tcaggtcatg accaaaatcc
     6181 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6241 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6301 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6361 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6421 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6481 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6601 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6841 acgcggcctt tttacgg
//
