LOCUS       sequence-327864-         532 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses an sgRNA targeting Zmat3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Bieging-Rolett KT, Kaiser AM, Morgens DW, Boutelle AM, Seoane JA, 
            Van Nostrand EL, Zhu C, Houlihan SL, Mello SS, Yee BA, McClendon J, 
            Pierce SE, Winters IP, Wang M, Connolly AJ, Lowe SW, Curtis C, Yeo 
            GW, Winslow MM, Bassik MC, Attardi LD
  TITLE     Zmat3 Is a Key Splicing Regulator in the p53 Tumor Suppression 
            Program.
  JOURNAL   Mol Cell. 2020 Nov 5;80(3):452-469.e9. doi: 
            10.1016/j.molcel.2020.10.022.
  PUBMED    33157015
REFERENCE   2  (bases 1 to 532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.10"; journalName: "Mol Cell"; date: 
            "2020-11-5- 5"; volume: "80"; issue: "3"; pages: "452-469.e9"
COMMENT     Sequence Label: pMCB320-sgZmat3.55.9
FEATURES             Location/Qualifiers
     source          1..532
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(6..39)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     43..63
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        306..517
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 tttctataac ttcgtatagc atacattata cgaagttata aacagcacaa aaggaaactc
       61 accctaactg taaagtaatt gtgtgttttg agactataag tatcccttgg agaaccacct
      121 tgttggctcc cctgccgtgg cacgtttaag agctaagctg gaaacagcat agcaagttta
      181 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctcg
      241 agtactagga tccattaggc ggccgcgtgg ataaccgtat taccgccatg catgtgcccg
      301 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
      361 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
      421 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
      481 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gt
//
