LOCUS       sequence-327982-        8781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of candidate TCR #5 TCRbeta cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Lee MN, Meyerson M
  TITLE     Antigen identification for HLA class I- and HLA class II-restricted 
            T cell receptors using cytokine-capturing antigen-presenting cells.
  JOURNAL   Sci Immunol. 2021 Jan 22;6(55). pii: 6/55/eabf4001. doi: 
            10.1126/sciimmunol.abf4001.
  PUBMED    33483338
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2021-01-22"; volume: "6(55). pii: 6/55/eabf4001. 
            doi"; pages: " 10.1126/sciimmunol.abf4001"
COMMENT     Sequence Label: CMVTCRb_candidate5
FEATURES             Location/Qualifiers
     source          1..8781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      606..615
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1592..2180
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1645..1665)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2063..2074)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2252..2485
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2563..2684
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2600..2619)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2654..2673
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2724..2859
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2879..2898)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2880..2898)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2908..2925)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2908..2924)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2917..2939)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3066..3521
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3153..3172)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3363..3384
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3547..3651
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3652..3720,3721..4512)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3870..3889)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4683..5271
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5172..5191
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5425..5442
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5559..5580
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5595..5600,5601..5618,5619..5625)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5633..5649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5638..5660
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5657..5673
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5657..5673
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5692..5712
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5694..5712
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5740..5966
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5967..6147
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6194..6319
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6812..7045
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7230..7274
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7449..7949
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7868..7886
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7971..8570
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7971..7990)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8467..8487
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8612..8729
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      601 ttgtcgccac catgggcttc aggctcctct gctgtgtggc cttttgtctc ctgggagcag
      661 gcccagtgga ttctggagtc acacaaaccc caaagcacct gatcacagca actggacagc
      721 gagtgacgct gagatgctcc cctaggtctg gagacctctc tgtgtactgg taccaacaga
      781 gcctggacca gggcctccag ttcctcattc agtattataa tggagaagag agagcaaaag
      841 gaaacattct tgaacgattc tccgcacaac agttccctga cttgcactct gaactaaacc
      901 tgagctctct ggagctgggg gactcagctt tgtatttctg tgccagcagc gtaaccggcg
      961 gcacagatac gcagtatttt ggcccaggca cccggctgac agtgctcgag gacctgaaaa
     1021 acgtgttccc acccgaggtc gctgtgtttg agccatcaga agcagagatc tcccacaccc
     1081 aaaaggccac actggtgtgc ctggccacag gcttcttccc tgaccacgtg gagctgagct
     1141 ggtgggtgaa tgggaaggag gtgcacagtg gggtctgcac ggacccgcag cccctcaagg
     1201 agcagcccgc cctcaatgac tccagatact gcctgagcag ccgcctgagg gtctcggcca
     1261 ccttctggca gaacccccgc aaccactttc gatgccaggt acaattttat ggtctctcgg
     1321 agaatgacga gtggacccag gatagggcca aacccgtcac ccagatcgtc agcgccgagg
     1381 cctggggtag agcagactgt ggctttacct cggtgtccta ccagcaaggg gtcctgtctg
     1441 ccacaatcct ctatgagatc ctgctaggga aggccaccct gtatgctgtg ctggtcagcg
     1501 cccttgtgtt gatggccatg gtcaagagaa aggatttctg aaaagtggtt tagtaatgag
     1561 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     1621 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     1681 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     1741 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     1801 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     1861 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     1921 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     1981 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2041 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2101 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2161 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     2221 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     2281 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     2341 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     2401 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     2461 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     2521 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     2581 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2641 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     2701 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     2761 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     2821 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     2881 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     2941 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3001 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3061 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3121 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3181 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3241 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3301 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3361 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3421 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3481 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     3541 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     3601 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     3661 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     3721 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     3781 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     3841 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     3901 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     3961 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4021 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4081 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4141 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4201 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4261 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4321 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     4381 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     4441 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     4501 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     4561 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     4621 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     4681 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     4741 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     4801 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     4861 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     4921 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     4981 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5041 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5101 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5161 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5221 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5281 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     5341 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     5401 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     5461 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     5521 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     5581 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     5641 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     5701 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     5761 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     5821 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     5881 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     5941 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     6001 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6061 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6121 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     6181 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     6241 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     6301 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     6361 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     6421 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     6481 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     6541 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     6601 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     6661 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     6721 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     6781 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     6841 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     6901 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     6961 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     7021 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     7081 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     7141 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     7201 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     7261 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     7321 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     7381 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     7441 ggggaccctt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     7501 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     7561 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     7621 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     7681 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
     7741 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
     7801 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
     7861 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
     7921 ttgaccgaat caccgacctc tctccccagg gggtaccacc ggagcttacc atgaccgagt
     7981 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     8041 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     8101 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     8161 tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag
     8221 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     8281 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     8341 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     8401 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     8461 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     8521 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gaattctaga
     8581 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     8641 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     8701 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     8761 tttggcgccg gctcgagggg g
//
