LOCUS       sequence-327985-       10640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of FLAG-tagged Tapasin delta3step.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10640)
  AUTHORS   McShan AC, Devlin CA, Morozov GI, Overall SA, Moschidi D, Akella N, 
            Procko E, Sgourakis NG
  TITLE     TAPBPR promotes antigen loading on MHC-I molecules using a peptide 
            trap.
  JOURNAL   Nat Commun. 2021 May 26;12(1):3174. doi: 10.1038/s41467-021-23225-6.
  PUBMED    34039964
REFERENCE   2  (bases 1 to 10640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-05-26"; volume: "12(1):3174. doi"; pages: " 
            10.1038/s41467-021-23225-6"
COMMENT     Sequence Label: pCEP4-FLAG-Tapasin delta3step
FEATURES             Location/Qualifiers
     source          1..10640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      633..642
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             717..740
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    2041..2175
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2065..2084)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2119..2138
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2318..2340)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6431..6449)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6517..6621
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6622..6690,6691..7482)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6840..6859)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7653..8241
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8142..8161
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8672..8817
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8860..9897
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(9895..9914)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9939..9987
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgccaccat ggccgtcatg gcgccccgaa
      661 ccctcgtcct gctactctcg ggggccctgg ccctgaccca gacctgggcg ggctccgact
      721 acaaggacga tgatgacaag ggaggatccg gccctgccgt aatcgagtgt tggtttgttg
      781 aaggtgctaa acggccaggc gcacttctgt tgcggcaagg tccgggagaa ccacctccac
      841 gacccgactt ggaccctgaa ttgtacctct ctgtacacga cccagctgga gcactccaag
      901 ctgctttccg acggtaccca agaggtgctc cagcaccgca ttgcgagatg agtcggttcg
      961 ttcctttgcc tgcgagtgca aagtgggcat caggtctgac accagcacaa aactgtccac
     1021 gagcgcttga tggagcgtgg ctgatggttt ccataagctc tccggtgctt tcactgtcat
     1081 cactgctcag gccacaacct gaaccccaac aagaacccgt actgataaca atggcgacgg
     1141 tagtccttac ggtccttacg catacaccag cacccagggt gcgattggga caagacgcac
     1201 tcctcgacct tagctttgcg tacatgccac caacctcaga ggcggcttcc tctcttgcac
     1261 caggaccacc accgttcggc cttgaatgga gacgacagca cttggggaag ggccatttgc
     1321 tgttggctgc taccccaggg ctgaacgggc agatgccggc agcacaagag ggagccgtag
     1381 cttttgctgc gtgggatgat gacgaaccat ggggaccatg gactggaaac gggactttct
     1441 ggctgccaac agtacagccc tttcaagaag gaacgtatct ggccaccatc catttgcctt
     1501 atctgcaagg ccaggtcaca cttgaactcg ctgtctataa accgcctaag gtgagtctca
     1561 tgcccgcaac tttggctagg gcagcacctg gcgaagctcc acctgaattg ctctgtttgg
     1621 tatcccattt ctaccccagt ggtggccttg aagtggaatg ggaacttaga ggaggaccag
     1681 gtggtcgatc tcagaaagct gaagggcagc gatggctctc cgctctcagg caccactcag
     1741 acggatctgt atcactgtca gggcatttgc agccaccgcc agttaccacc gagcagcatg
     1801 gagctcggta tgcgtgtcgc attcaccacc ctagccttcc ggcgtcagga agatcagcgg
     1861 aggtaacact tgaggtcgct gggttgtccg gacccagctt ggaggacagt gttggtctgt
     1921 tcctgtccgc tttcctgttg ctgggtctgt ttaaggcact cggatgggca gcagtttatc
     1981 tcagcacctg taaagacagc aagaaaaagg cagaatagta actcgaggcc ggcaaggccg
     2041 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     2101 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     2161 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     2221 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     2281 ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     2341 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     2401 cagcgggtgt tggcgggtgt cggggcgcag ccatgaggtc gactctagag gatcgatccc
     2461 cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg gggcagtgca
     2521 tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca catgtgacac
     2581 ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta taaatggatg
     2641 tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag tctgtggact
     2701 gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc aatgctgggg
     2761 gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg tcaatcagag
     2821 gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata gtgtttataa
     2881 ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt atatactatc
     2941 cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc tatcgaatta
     3001 gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc tgtcacggtt
     3061 ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca caagggcagt
     3121 ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct tcttcattct
     3181 ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta tgtgaggtgc
     3241 tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg gttcagtggt
     3301 ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata aatactagtg
     3361 taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg ggacaagccg
     3421 taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat aatcctagtg
     3481 caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca ggtgaaccat
     3541 gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc agcggactcc
     3601 actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg gggcccataa
     3661 acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg cgtcagcccc
     3721 cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg gctgattgta
     3781 accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg caccccgggg
     3841 aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc gatctggagg
     3901 acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc tcatattcac
     3961 gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact acccaaatat
     4021 ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc taatctatat
     4081 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatytatat
     4141 ctgggtagya takgctatcc taatytatat ctgggtagca taggctatcc taatctatat
     4201 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     4261 ccgggtagca tatgctatcc tcatgcatat acagtcagca tatgataccc agtagtagag
     4321 tgggagtgct atcctttgca tatgccgcca cctcccaagg gggcgtgaat tttcgctgct
     4381 tgtccttttc ctgctggttg ctcccattct taggtgaatt taaggaggcc aggctaaagc
     4441 cgtcgcatgt ctgattgctc accaggtaaa tgtcgctaat gttttccaac gcgagaaggt
     4501 gttgagcgcg gagctgagtg acgtgacaac atgggtatgc ccaattgccc catgttggga
     4561 ggacgaaaat ggtgacaaga cagatggcca gaaatacacc aacagcacgc atgatgtcta
     4621 ctggggattt attctttagt gcgggggaat acacggcttt taatacgatt gagggcgtct
     4681 cctaacaagt tacatcactc ctgcccttcc tcaccctcat ctccatcacc tccttcatct
     4741 ccgtcatctc cgtcatcacc ctccgcggca gccccttcca ccataggtgg aaaccaggga
     4801 ggcaaatcta ctccatcgtc aaagctgcac acagtcaccc tgatattgca ggtaggagcg
     4861 ggctttgtca taacaaggtc cttaatcgca tccttcaaaa cctcagcaaa tatatgagtt
     4921 tgtaaaaaga ccatgaaata acagacaatg gactccctta gcgggccagg ttgtgggccg
     4981 ggtccagggg ccattccaaa ggggagacga ctcaatggtg taagacgaca ttgtggaata
     5041 gcaagggcag ttcctcgcct taggttgtaa agggaggtct tactacctcc atatacgaac
     5101 acaccggcga cccaagttcc ttcgtcggta gtcctttcta cgtgactcct agccaggaga
     5161 gctcttaaac cttctgcaat gttctcaaat ttcgggttgg aacctccttg accacgatgc
     5221 tttccaaacc accctccttt tttgcgcctg cctccatcac cctgaccccg gggtccagtg
     5281 cttgggcctt ctcctgggtc atctgcgggg ccctgctcta tcgctcccgg gggcacgtca
     5341 ggctcaccat ctgggccacc ttcttggtgg tattcaaaat aatcggcttc ccctacaggg
     5401 tggaaaaatg gccttctacc tggagggggc ctgcgcggtg gagacccgga tgatgatgac
     5461 tgactactgg gactcctggg cctcttttct ccacgtccac gacctctccc cctggctctt
     5521 tcacgacttc cccccctggc tctttcacgt cctctacccc ggcggcctcc actacctcct
     5581 cgaccccggc ctccactacc tcctcgaccc cggcctccac tgcctcctcg accccggcct
     5641 ccacctcctg ctcctgcccc tcctgctcct gcccctcctc ctgctcctgc ccctcctgct
     5701 cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg
     5761 caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag
     5821 ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca
     5881 tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg
     5941 tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt
     6001 cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg
     6061 cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga
     6121 cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac
     6181 tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca
     6241 ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc
     6301 ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct
     6361 gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga
     6421 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     6481 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6541 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     6601 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     6661 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     6721 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     6781 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     6841 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     6901 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     6961 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     7021 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     7081 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     7141 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     7201 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     7261 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     7321 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     7381 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     7441 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     7501 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     7561 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7621 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7681 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7741 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     7801 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7861 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7921 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7981 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8041 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8101 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8161 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     8221 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     8281 tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     8341 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     8401 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg
     8461 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
     8521 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     8581 ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
     8641 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
     8701 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca
     8761 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag
     8821 cgtcaacagc gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
     8881 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     8941 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     9001 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     9061 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     9121 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     9181 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     9241 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     9301 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     9361 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     9421 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     9481 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     9541 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     9601 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     9661 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     9721 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     9781 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     9841 agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac
     9901 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     9961 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
    10021 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
    10081 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
    10141 gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt
    10201 atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga
    10261 gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac
    10321 gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt
    10381 tctggcgtgc caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg
    10441 ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt
    10501 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
    10561 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
    10621 tccaatatga ccgccatgtt
//
